Structural Characterization of Recombinant Hepatitis E Virus ORF2 Proteins in Baculovirus-Infected Insect Cells

1998 ◽  
Vol 12 (1) ◽  
pp. 75-84 ◽  
Author(s):  
Robin A. Robinson ◽  
Wilson H. Burgess ◽  
Suzanne U. Emerson ◽  
Rande S. Leibowitz ◽  
Svetlana A. Sosnovtseva ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Hepatitis E Virus ◽  
Insect Cells ◽  
Hepatitis E ◽  
Recombinant Hepatitis ◽  
Infected Insect

Robust manufacturing and comprehensive characterization of recombinant hepatitis E virus-like particles in Hecolin®

Vaccine ◽  
2014 ◽  
Vol 32 (32) ◽  
pp. 4039-4050 ◽  
Author(s):  
Xiao Zhang ◽  
Minxi Wei ◽  
Huirong Pan ◽  
Zhijie Lin ◽  
Kaihang Wang ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Recombinant Hepatitis ◽  
Virus Like Particles ◽  
Robust Manufacturing ◽  
Comprehensive Characterization

Expression and characterization of hepatitis E virus-like particles and non-virus-like particles from insect cells

2015 ◽  
Vol 63 (3) ◽  
pp. 362-370 ◽  
Author(s):  
Ying Qi ◽  
Jinping Fan ◽  
Weijin Huang ◽  
Chenyan Zhao ◽  
Youchun Wang ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Insect Cells ◽  
Hepatitis E ◽  
Virus Like Particles

scholarly journals Antigenic Characterization of ORF2 and ORF3 Proteins of Hepatitis E Virus (HEV)

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1385
Author(s):  
Giulia Pezzoni ◽  
Lidia Stercoli ◽  
Eleonora Pegoiani ◽  
Emiliana Brocchi
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Recombinant Antigen ◽  
Open Reading Frame ◽  
Reading Frame ◽  
E Coli ◽  
Antigenic Characterization ◽  
Antigenic Properties ◽  
Open Reading Frame 2

To evaluate the antigenic properties of Hepatitis E Virus (HEV) Open Reading Frame 2 and 3 (ORF2 and ORF3) codified proteins, we expressed different portions of ORF2 and the entire ORF3 in E. coli, a truncated ORF2, was also expressed in baculovirus. A panel of 37 monoclonal antibodies (MAbs) was raised against ORF2 (1–660 amino acids) and MAbs were mapped and characterized using the ORF2 expressed portions. Selected HEV positive and negative swine sera were used to evaluate ORF2 and ORF3 antigens’ immunogenicity. The MAbs were clustered in six groups identifying six antigenic regions along the ORF2. Only MAbs binding to the sixth ORF2 antigenic region (394–608 aa) were found to compete with HEV positive sera and efficiently catch the recombinant antigen expressed in baculovirus. The ORF2 portion from 394–608 aa demonstrated to include most immunogenic epitopes with 85% of HEV positive swine sera reacting against the region from 461–544 aa. Only 5% of the selected HEV sera reacted against the ORF3 antigen.

Expression and Characterization of Human Dopamine D2Receptor in Baculovirus-Infected Insect Cells

1994 ◽  
Vol 14 (2) ◽  
pp. 99-117 ◽  
Author(s):  
J. A. Javitch ◽  
J. Kaback ◽  
X. Li ◽  
A. Karlin
Keyword(s):  
Insect Cells ◽  
Infected Insect

DETECTION AND PHYLOGENETIC CHARACTERIZATION OF HEPATITIS E VIRUS GENOTYPE 3 IN A CAPTIVE WILD BOAR IN THAILAND

2012 ◽  
Vol 43 (3) ◽  
pp. 640-644 ◽  
Author(s):  
Anuwat Wiratsudakul ◽  
Ladawan Sariya ◽  
Phirom Prompiram ◽  
Siriporn Tantawet ◽  
Duangkhamol Suraruangchai ◽  
...  
Keyword(s):  
Wild Boar ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genotype 3 ◽  
Virus Genotype ◽  
Phylogenetic Characterization

scholarly journals Prevalence and Molecular Characterization of the Hepatitis E Virus in Retail Pork Products Marketed in Canada

2017 ◽  
Vol 9 (2) ◽  
pp. 208-218 ◽  
Author(s):  
Oksana Mykytczuk ◽  
Jennifer Harlow ◽  
Sabah Bidawid ◽  
Nathalie Corneau ◽  
Neda Nasheri
Keyword(s):  
Molecular Characterization ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Pork Products

Identification and characterization of avian hepatitis E virus genotype 2 from chickens with hepatitis-splenomegaly syndrome in Korea

Virus Genes ◽  
2016 ◽  
Vol 52 (5) ◽  
pp. 738-742 ◽  
Author(s):  
Hyun-Woo Moon ◽  
Byung-Woo Lee ◽  
Haan Woo Sung ◽  
Byung-Il Yoon ◽  
Hyuk Moo Kwon
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Genotype ◽  
Genotype 2 ◽  
Avian Hepatitis E Virus ◽  
Identification And Characterization

Characterization of rabbit hepatitis E virus isolated from a feral rabbit

2021 ◽  
pp. 109275
Author(s):  
Milagros Virhuez Mendoza ◽  
Kenzo Yonemitsu ◽  
Keita Ishijima ◽  
Shohei Minami ◽  
Supriyono ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E
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