Expression and Purification of the Epidermal Growth Factor Receptor Extracellular Domain Utilizing a Polycistronic Expression System

1993 ◽  
Vol 4 (3) ◽  
pp. 177-186 ◽  
Author(s):  
D.L. Cadena ◽  
G.N. Gill
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Expression System ◽  
Growth Factor Receptor ◽  
Extracellular Domain ◽  
Expression And Purification ◽  
Epidermal Growth

scholarly journals Extracellular domain of HER2 heterologous expression in bacteria

2013 ◽  
Vol 20 (4) ◽  
pp. 70-73
Author(s):  
V. V. Dolgikh ◽  
I. V. Senderskiy, ◽  
G. V. Tetz ◽  
V. V. Tetz
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Growth Factor Receptor ◽  
Extracellular Domain ◽  
Breast Cancers ◽  
Expression And Purification ◽  
Oncological Diseases ◽  
Epidermal Growth ◽  
Sequence Encoding

Human epidermal growth factor receptor (HER2/neu, c ErbB2) is a protooncogene protein which is overexpressed in some oncological diseases including 30 % of breast cancers, tumors in the ovary, stomach and other organs of the human body as well. Since Her2tumor status testing is the essential part of successful cancer treatment, expression and purification of substantial amounts of the extracellular domain of the ECD HER2 is an important task. In this work sequence encoding HER2 extracellular domain was cloned and expressed in E.coli.

scholarly journals A point mutation in the extracellular domain activates LET-23, the Caenorhabditis elegans epidermal growth factor receptor homolog.

1996 ◽  
Vol 16 (2) ◽  
pp. 529-537 ◽  
Author(s):  
W S Katz ◽  
G M Lesa ◽  
D Yannoukakos ◽  
T R Clandinin ◽  
J Schlessinger ◽  
...  
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Caenorhabditis Elegans ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Point Mutation ◽  
Growth Factor Receptor ◽  
Extracellular Domain ◽  
Cysteine Residue ◽  
Loss Of Function ◽  
Epidermal Growth

The let-23 gene encodes a Caenorhabditis elegans homolog of the epidermal growth factor receptor (EGFR) necessary for vulval development. We have characterized a mutation of let-23 that activates the receptor and downstream signal transduction, leading to excess vulval differentiation. This mutation alters a conserved cysteine residue in the extracellular domain and is the first such point mutation in the EGFR subfamily of tyrosine kinases. Mutation of a different cysteine in the same subdomain causes a strong loss-of-function phenotype, suggesting that cysteines in this region are important for function and nonequivalent. Vulval precursor cells can generate either of two subsets of vulval cells (distinct fates) in response to sa62 activity. The fates produced depended on the copy number of the mutation, suggesting that quantitative differences in receptor activity influence the decision between these two fates.

scholarly journals Interaction of the Extracellular Domain of the Epidermal Growth Factor Receptor with Gangliosides

2002 ◽  
Vol 277 (12) ◽  
pp. 10108-10113 ◽  
Author(s):  
Erik A. Miljan ◽  
Emmanuelle J. Meuillet ◽  
Barbara Mania-Farnell ◽  
David George ◽  
Hirotaka Yamamoto ◽  
...  
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Growth Factor Receptor ◽  
Extracellular Domain ◽  
Epidermal Growth
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