Localization of Zucchini yellow mosaic virus to the veinal regions and role of viral coat protein in veinal chlorosis conditioned by the zym potyvirus resistance locus in cucumber

2002 ◽  
Vol 60 (2) ◽  
pp. 79-89 ◽  
Author(s):  
Zakir Ullah ◽  
Rebecca Grumet
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Resistance Locus ◽  
Viral Coat Protein ◽  
Viral Coat

scholarly journals Cis-Preferential Stimulation of Alfalfa Mosaic Virus RNA 3 Accumulation by the Viral Coat Protein

Virology ◽  
1996 ◽  
Vol 220 (1) ◽  
pp. 163-170 ◽  
Author(s):  
E.A.G. van der VOSSEN ◽  
C.B.E.M. REUSKEN ◽  
J.F. BOL
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Alfalfa Mosaic Virus ◽  
Viral Coat Protein ◽  
Virus Rna ◽  
Viral Coat ◽  
Stimulation Of

scholarly journals Role of Recombination in the Evolution of Host Specialization Within Bean yellow mosaic virus

2009 ◽  
Vol 99 (5) ◽  
pp. 512-518 ◽  
Author(s):  
S. J. Wylie ◽  
R. A. C. Jones
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Genetic Recombination ◽  
Bean Yellow Mosaic Virus ◽  
Host Specialization ◽  
Yellow Mosaic Virus ◽  
Complete Genomes ◽  
Tentative Evidence ◽  
Firm Evidence

Seven complete genomes and 64 coat protein gene sequences belonging to Bean yellow mosaic virus (BYMV) isolates from different continents were examined for evidence of genetic recombination using six different recombination-detection programs. In the seven complete genomes and a single complete genome of the related virus Clover yellow vein virus (ClYVV), evidence for eight recombination patterns was found by four or more programs, giving firm evidence of their presence, and five additional recombination patterns were detected by three or fewer programs, giving tentative evidence of their occurrence. When the nucleotide sequences of 64 BYMV and one ClYVV coat protein genes were analyzed, three firm recombination patterns were detected in 21 isolates (32%). With another six isolates (9%), tentative evidence was found for three further recombination patterns. Of the 19 firm or tentative recombination patterns detected within and between strain groups of BYMV, and with ClYVV, 12 involved a generalist group of isolates as a parent but none of the other BYMV groups acted as parents more than six times. These findings suggest that recombination played an important role in the evolution of BYMV strain groups that specialize in infecting particular groups of domesticated plants.

scholarly journals Maintenance of coat protein N-terminal net charge and not primary sequence is essential for zucchini yellow mosaic virus systemic infectivity

2004 ◽  
Vol 85 (11) ◽  
pp. 3421-3430 ◽  
Author(s):  
Boaz Kimalov ◽  
Amit Gal-On ◽  
Ran Stav ◽  
Eduard Belausov ◽  
Tzahi Arazi
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Cell Movement ◽  
Yellow Mosaic Virus ◽  
Virus Infectivity ◽  
Amino Acid Residues ◽  
Primary Sequence ◽  
Net Charge ◽  
Truncation Mutant

Zucchini yellow mosaic virus (ZYMV) surface exposed coat protein (CP) N-terminal domain (Nt) is 43 aa long and contains an equal number of positively and negatively charged amino acid residues (CP-Nt net charge=0). A ZYMV-AGII truncation mutant lacking the first 20 aa of its CP-Nt (AGII-CPΔ20; CP-Nt net charge=+2) was found to be systemically non-infectious even though AGII mutants harbouring larger CP-Nt deletions were previously demonstrated to be fully infectious. Nevertheless, AGII-CPΔ20 infectivity was restored by fusion to its CP-Nt two Asp residues or a negatively charged Myc peptide, both predicted to neutralize CP-Nt net positive charge. To evaluate further the significance of CP-Nt net charge for AGII infectivity, a series of CP-Nt net charge mutants was generated and analysed for systemic infectivity of squash plants. AGII-CPKKK harbouring a CP-Nt amino fusion of three Lys residues (CP-Nt net charge=+3) was not systemically infectious. Addition of up to four Asp residues to CP-Nt did not abolish virus infectivity, although certain mutants were genetically unstable and had delayed infectivity. Addition of five negatively charged residues abolished infectivity (AGII-CPDDDDD; CP-Nt net charge=−5) even though a recombinant CPDDDDD could assemble into potyviral-like particle in bacteria. Neutralization of CP-Nt net charge by fusing Asp or Lys residues recovered infectivity of AGII-CPKKK and AGII-CPDDDDD. GFP-tagging of these mutants has demonstrated that both viruses have defective cell-to-cell movement. Together, these findings suggest that maintenance of CP-Nt net charge and not primary sequence is essential for ZYMV infectivity.

scholarly journals Characterization of Two Unusual Features of Resistance to Soilborne cereal mosaic virus in Hexaploid Wheat: Leakiness and Gradual Elimination of Viral Coat Protein from Infected Root Tissues

2009 ◽  
Vol 22 (5) ◽  
pp. 560-574 ◽  
Author(s):  
Rebecca Lyons ◽  
Nazli D. Kutluk Yilmaz ◽  
Stephen Powers ◽  
Kim E. Hammond-Kosack ◽  
Kostya Kanyuka
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Incidence Rate ◽  
Viral Rna ◽  
Separate Experiment ◽  
Viral Coat Protein ◽  
Resistant Cultivars ◽  
Short Period ◽  
Resistance Breakdown ◽  
Viral Coat

Spatiotemporal infection patterns of Soilborne cereal mosaic virus (SBCMV) were compared between resistant and susceptible wheat cultivars to elucidate disease resistance mechanisms. Resistance to SBCMV was manifested by a gradual disappearance of the viral coat protein (CP) from the roots following an initial short period of steady accumulation. Interestingly, viral RNA persisted in the roots of resistant cultivars even after the CP had disappeared. Traces of viral RNA were also detected in the uninoculated leaves of the resistant cv. Cadenza. These findings suggest that the resistance mechanism to SBCMV in wheat involves the efficient disassembly of virus particles and either an inhibition of further synthesis of viral CP or its proteolytic degradation. SBCMV accumulated in the leaves of a small proportion of individual plants of Cadenza and other recognized resistant cultivars, highlighting the leaky nature of the resistance, but the roots of these plants were often devoid of viral CP. Increasing or decreasing the concentration of the inocula had no effect on the incidence rate of such “resistance breakdown”; however, a positive correlation was found between the incidence rate of resistance breakdown and the percentage of systemically infected individuals of recognized susceptible cultivars in each separate experiment.

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