Mechanical wounding of potato tubers induces replication of potato virus S

1996 ◽  
Vol 49 (1) ◽  
pp. 33-47 ◽  
Author(s):  
J.K. Morelli ◽  
M.E. Vayda
1974 ◽  
Vol 52 (3) ◽  
pp. 461-465 ◽  
Author(s):  
J. P. MacKinnon

Seventy-two potato tubers of 106 tested from plants exposed 1 year in a field were found infected with potato virus S (PVS) in different tests. Ninety-three percent of these were detected by tuber juice inoculation to Nicotiana debneyi Domin. and 90% by serology of 30-cm plants grown from an eye of such tubers. Sap inoculation to N. debneyi of the same young plants proved to be 96% efficient in detecting the virus, and serological tests at bloom stage were the most efficient of all the tests compared.Tests done on all tubers from 18 plants currently infected with PVS showed that 103 of 116 (89%) were infected, and virtually all eyes from 68 infected tubers produced infected plants.Three years of field trials at Fredericton on the spread of PVS showed that the virus moved into virus-free varieties independently of potato virus X (PVX). In 1970, leaf tests showed that virus-free Netted Gems became 12% infected with PVS; in 1971, spread into Green Mountain, Kennebec, and Sebago was 57, 19, and 9%, respectively; and in 1972, 14% spread occurred in Green Mountain and none in Kennebec or Sebago.Greenhouse experiments on transmission of PVS to potato by Myzus persicae (Sulz.) resulted in 3 of 87 (3.4%) plants becoming infected. Other tests with potato virus Y (PVY) to tobacco, Nicotiana tabacum L. var. Samsun, resulted in 83% transmission.


1976 ◽  
Vol 19 (2) ◽  
pp. 131-139 ◽  
Author(s):  
A. Kowalska ◽  
M. Waś

2007 ◽  
Vol 8 (1) ◽  
pp. 70 ◽  
Author(s):  
Susan J. Lambert ◽  
Frank S. Hay ◽  
Sarah J. Pethybridge ◽  
Calum R. Wilson

The spatial and temporal distribution of Potato virus S (PVS) and Potato virus X (PVX) was studied in two trials within each of four commercial fields of seed potato var. Russet Burbank in Tasmania, Australia. In the first trial (plots) 20 leaflets were collected from each of 49 plots (each approximately 8 m wide by 10 m long), with plots arranged in a 7-×-7 lattice. In the second trial (transects), leaflets were collected at 1-m intervals along seven adjacent, 50-m long rows. The mean incidence of PVS increased during the season by 5.2% in one of four plot trials and 25.5% in one of four transect trials. The mean incidence of PVX increased during the season by 10.1%, in one of two transect trials. Spatial Analysis by Distance IndicEs and ordinary runs analysis detected aggregation of PVS infected plants early in the season in one and two fields respectively, suggesting transmission during seed-cutting or during planting. An increase in PVS incidence mid- to late season in one field was associated with aggregation of PVS along, but not across rows, which may be related to the closer plant spacing within rows and hence increased potential for mechanical transmission along rows. Results suggested limited spread of PVS and PVX occurred within crops during the season. Accepted for publication 9 April 2007. Published 26 July 2007.


Virus Genes ◽  
1992 ◽  
Vol 6 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Gary D. Foster ◽  
Peter R. Mills

2018 ◽  
Vol 54 (No. 1) ◽  
pp. 30-33 ◽  
Author(s):  
M. Naderpour ◽  
L. Sadeghi

Molecular markers within or close to genes of interest play essential roles in marker-assisted selection. PCR-based markers have been developed for numerous traits in different plant species including several genes conferring resistance to viruses in potato. In the present work, rapid and reliable approaches were developed for the simultaneous detection of Ryadg and Ry-fsto, Ns, and PLRV.1 genes conferring resistance to Potato virus Y, Potato virus S and Potato leafroll virus, respectively, on the basis of previously published and newly modified markers. The sequence characterized amplified region (SCAR) markers for Ryadg, Ns and PLRV1 and the newly modified cleaved amplified polymorphic sequences (CAPS) marker for Ry-fsto were amplified in one PCR reaction which could simply characterize Ryadg and PLRV.1 resistance. Additional digestion of amplicons with EcoRV and MfeI for genotyping the Ry-fsto and Ns resistance genes, respectively, was needed. The effectiveness of genotyping in triplex and tetraplex PCRs was tested on 35 potato varieties used for potato seed production and breeding programs.  


2013 ◽  
Vol 158 (10) ◽  
pp. 2205-2208 ◽  
Author(s):  
Pablo A. Gutiérrez ◽  
Juan Fernando Alzate ◽  
Mauricio A. Marín-Montoya

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