Completion of the Nucleotide Sequence of the Enterococcus faecalis Conjugative Virulence Plasmid pAD1 and Identification of a Second Transfer Origin

Plasmid ◽  
2001 ◽  
Vol 46 (2) ◽  
pp. 117-127 ◽  
Author(s):  
M.Victoria Francia ◽  
Wolfgang Haas ◽  
Reinhard Wirth ◽  
Elke Samberger ◽  
Albrecht Muscholl-Silberhorn ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Enterococcus Faecalis ◽  
Virulence Plasmid ◽  
Transfer Origin

scholarly journals Molecular analysis of enterolysin a and entL gene cluster from natural isolate Enterococcus faecalis BGPT1-10P

Genetika ◽  
2013 ◽  
Vol 45 (2) ◽  
pp. 479-492 ◽  
Author(s):  
Katarina Veljovic ◽  
Amarela Terzic-Vidojevic ◽  
Maja Tolinacki ◽  
Milan Kojic ◽  
Ljubisa Topisirovic
Keyword(s):  
Functional Analysis ◽  
Nucleotide Sequence ◽  
Enterococcus Faecalis ◽  
Broad Spectrum ◽  
Candida Species ◽  
Genetic Information ◽  
Natural Isolate ◽  
Gene Encoding ◽  
Dna Fragment ◽  
Virulent Factor

Strain Enterococcus faecalis BGPT1-10P was isolated from artisanal semi-hard homemade cheese from Stara Planina, Serbia. Results showed that BGPT1-10P synthesized a heat labile bacteriocin with a broad spectrum of activity, including Listeria and Candida species. Further analysis revealed that synthesized bacteriocin is enterolysin A. Moreover, the entL gene encoding enterolysin A was found to be located on the chromosome. The entL gene was cloned and sequenced. Analysis of nucleotide sequence showed that the entL gene in natural isolate En. faecalis BGPT1-10P is identical to that of the entL gene described previously in En. faecalis LMG 2333. Within the cloned DNA fragment containing the entL gene, four ORFs were detected. One of them was identified as the scpE gene, which encodes a virulent factor staphopain peptidase. Functional analysis of the entL gene showed that the complete genetic information necessary for the synthesis of enterolysin A were directly linked solely to it. Strain BGPT1-10P also synthesized gelatinase and citolysin, and contained a set of virulent factors. In addition, BGPT1-10P carries the ermB and tetM genes conferring the resistance to erythromycin and tetracycline, respectively.

Genetic map of the virulence plasmid of Salmonella enteritidis and nucleotide sequence of its replicons

Gene ◽  
1997 ◽  
Vol 188 (1) ◽  
pp. 53-61 ◽  
Author(s):  
José M Rodrı́guez-Peña ◽  
Miguel Buisán ◽  
Magdalena Ibáñez ◽  
Rafael Rotger
Keyword(s):  
Nucleotide Sequence ◽  
Genetic Map ◽  
Salmonella Enteritidis ◽  
Virulence Plasmid

scholarly journals Yersinia pseudotuberculosis Produces a Cytotoxic Necrotizing Factor

2002 ◽  
Vol 70 (5) ◽  
pp. 2708-2714 ◽  
Author(s):  
Hank A. Lockman ◽  
Rebecca A. Gillespie ◽  
Beth D. Baker ◽  
Elizabeth Shakhnovich
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Yersinia Pseudotuberculosis ◽  
Virulence Plasmid ◽  
E Coli ◽  
Cytotoxic Necrotizing Factor ◽  
Cell Extracts

ABSTRACT Cell extracts from Yersinia pseudotuberculosis induced multinucleation in HEp-2 cells in a manner similar to the effect caused by Escherichia coli cytotoxic necrotizing factor (CNF). The activity was not dependent on the Yersinia 70-kb virulence plasmid, and the activity was not inhibited by antibodies capable of neutralizing E. coli CNF type 1. The nucleotide sequence of the Yersinia cnf gene was 65.1% identical to the E. coli cnf gene.

scholarly journals Complete nucleotide sequence of pRS218, a large virulence plasmid, that augments pathogenic potential of meningitis-associated Escherichia coli strain RS218

2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Dona Saumya S Wijetunge ◽  
Kurundu Hewage Eranda M Karunathilake ◽  
Atul Chaudhari ◽  
Robab Katani ◽  
Edward G Dudley ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Complete Nucleotide Sequence ◽  
Coli Strain ◽  
Virulence Plasmid ◽  
Pathogenic Potential

scholarly journals MOLECULAR-GENETIC CHARACTERISTICS OF SHIGELLA SONNEI-2013 STRAIN ISOLATED DURING THE OUTBREAK IN DYSENTERY IN THE REPUBLIC ABKHAZIA IN 2013

2018 ◽  
pp. 72-76
Author(s):  
O. V. Vasileva ◽  
A. S. Volynkina ◽  
I. V. Kuznetsova ◽  
S. V. Pisarenko ◽  
A. N. Kulichenko
Keyword(s):  
Nucleotide Sequence ◽  
Molecular Genetic ◽  
Housekeeping Genes ◽  
Shigella Sonnei ◽  
Personal Genome ◽  
Virulence Plasmid ◽  
Genetic Characteristics ◽  
E Coli ◽  
Type Iv ◽  
The Republic

Aim. Study of molecular-genetic properties of Shigella sonnei-2013 strain isolated during the outbreak in dysentery in the republic Abkhazia in 2013. Materials and methods. Genetic typing of the tested strains using multilocus sequence typing (MLST). Analyzed of nucleotide sequence fragments 7 of conservative «housekeeping» genes adk, fumC, icd, mdh, purA, recA, gyrB. Sequenced of DNA fragments compared with reference sequences from database of Escherichia coli MLST. Phylogenetic analysis was performed using UPGMA method and computer program START 2. Whole-genome sequencing performed on a genetic analyzer Ion Torrent Personal Genome Machine (PGM™) using fragment libraries (shot-gun). Aligning reads have been carried out with the program GS Reference Mapper. Results. Defined sequence - type of the studied strain - ST-152, one of the most common genotypes for S. sonnei. Demonstrated the high degree of similarity obtained contig to the sequences of the chromosome and plasmids А, В, С и E strains S. sonnei 53G and S. sonnei Ss046. Identified contigs with a high percentage similarity to the sequence of virulence plasmid p026-Vir of E. coli 026:H11 (H30). In the genomic S. sonnei-2013 revealed nucleotide sequence of 136 genes were found located on the p026-Vir strain of E. coli 026:Н11 (НЗО). Discovered genes controlling biosynthesis of type IV pili involved in adhesion to abiotic surfaces and biofilm formation. Conclusion. Identified structural peculiarities of strain induced by fragments of virulence plasmid p026-Vir strain E. coli 026:H 11 (H30).

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