Sequence Analysis of the Plasmid pRRI2 from the Rumen Bacterium Prevotella ruminicola 223/M2/7 and the Use of pRRI2 in Prevotella/Bacteroides Shuttle Vectors

Plasmid ◽  
2001 ◽  
Vol 45 (3) ◽  
pp. 227-232 ◽  
Author(s):  
Derry K. Mercer ◽  
Saleem Patel ◽  
Harry J. Flint
Keyword(s):  
Sequence Analysis ◽  
Rumen Bacterium ◽  
Shuttle Vectors ◽  
Prevotella Ruminicola

Characterization of plasmids from human infant Bifidobacterium strains: Sequence analysis and construction of E. coli–Bifidobacterium shuttle vectors

Plasmid ◽  
2008 ◽  
Vol 60 (2) ◽  
pp. 136-148 ◽  
Author(s):  
Andrei N. Shkoporov ◽  
Boris A. Efimov ◽  
Ekaterina V. Khokhlova ◽  
James L. Steele ◽  
Lyudmila I. Kafarskaia ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Human Infant ◽  
Shuttle Vectors ◽  
E Coli

scholarly journals Biochemical Characterization and Relative Expression Levels of Multiple Carbohydrate Esterases of the Xylanolytic Rumen Bacterium Prevotella ruminicola 23 Grown on an Ester-Enriched Substrate

2011 ◽  
Vol 77 (16) ◽  
pp. 5671-5681 ◽  
Author(s):  
Mirjam A. Kabel ◽  
Carl J. Yeoman ◽  
Yejun Han ◽  
Dylan Dodd ◽  
Charles A. Abbas ◽  
...  
Keyword(s):  
Ferulic Acid ◽  
Biochemical Characterization ◽  
Corn Fiber ◽  
Rumen Bacterium ◽  
Transcriptional Level ◽  
Content Type ◽  
Carbohydrate Esterase ◽  
Prevotella Ruminicola ◽  
Acetyl Esterases ◽  
Carbohydrate Esterases

ABSTRACTWe measured expression and used biochemical characterization of multiple carbohydrate esterases by the xylanolytic rumen bacteriumPrevotella ruminicola23 grown on an ester-enriched substrate to gain insight into the carbohydrate esterase activities of this hemicellulolytic rumen bacterium. TheP. ruminicola23 genome contains 16 genes predicted to encode carbohydrate esterase activity, and based on microarray data, four of these were upregulated >2-fold at the transcriptional level during growth on an ester-enriched oligosaccharide (XOSFA,Ac) from corn relative to a nonesterified fraction of corn oligosaccharides (AXOS). Four of the 16 esterases (Xyn10D-Fae1A, Axe1-6A, AxeA1, and Axe7A), including the two most highly induced esterases (Xyn10D-Fae1A and Axe1-6A), were heterologously expressed inEscherichia coli, purified, and biochemically characterized. All four enzymes showed the highest activity at physiologically relevant pH (6 to 7) and temperature (30 to 40°C) ranges. TheP. ruminicola23 Xyn10D-Fae1A (a carbohydrate esterase [CE] family 1 enzyme) released ferulic acid from methylferulate, wheat bran, corn fiber, and XOSFA,Ac, a corn fiber-derived substrate enriched inO-acetyl and ferulic acid esters, but exhibited negligible activity on sugar acetates. As expected, theP. ruminicolaAxe1-6A enzyme, which was predicted to possess two distinct esterase family domains (CE1 and CE6), released ferulic acid from the same substrates as Xyn10D-Fae1 and was also able to cleaveO-acetyl ester bonds from various acetylated oligosaccharides (AcXOS). TheP. ruminicola23 AxeA1, which is not assigned to a CE family, and Axe7A (CE7) were found to be acetyl esterases that had activity toward a broad range of mostly nonpolymeric acetylated substrates along with AcXOS. All enzymes were inhibited by the proximal location of other side groups like 4-O-methylglucuronic acid, ferulic acid, or acetyl groups. The unique diversity of carbohydrate esterases inP. ruminicola23 likely gives it the ability to hydrolyze substituents on the xylan backbone and enhances its capacity to efficiently degrade hemicellulose.

Peptidases of the Rumen Bacterium,Prevotella ruminicola

Anaerobe ◽  
1997 ◽  
Vol 3 (1) ◽  
pp. 35-42 ◽  
Author(s):  
R.John Wallace ◽  
Nest McKain ◽  
Glen A Broderick ◽  
Lyle M Rode ◽  
Nicola D Walker ◽  
...  
Keyword(s):  
Rumen Bacterium ◽  
Prevotella Ruminicola

Phylogeny and taxonomy of the family Arthrodermataceae (dermatophytes) using sequence analysis of the ribosomal ITS region

1999 ◽  
Vol 37 (2) ◽  
pp. 105-114 ◽  
Author(s):  
Y. GRAser ◽  
M. EL Fari ◽  
R. Vilgalys ◽  
A. F. A. Kuijpers ◽  
G. S. DE Hoog ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Its Region ◽  
The Family
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