Effects of some medical drugs on enzyme activities of carbonic anhydrase from human erythrocytes in vitro and from rat erythrocytes in vivo

2000 ◽  
Vol 42 (2) ◽  
pp. 187-191 ◽  
Author(s):  
Şükrü Beydemir ◽  
Mehmet Çİftçi ◽  
İsmail Özmen ◽  
Mehmet Emin Buyuk Okuroglu ◽  
Hasan Özdemir ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Enzyme Activities ◽  
Human Erythrocytes ◽  
Rat Erythrocytes

scholarly journals Effects of Gentamicin Sulfate on Enzyme Activities of Carbonic Anhydrase from Human Erythrocytes in Vitro and from Rat Erythrocytes in Vivo

2002 ◽  
Vol 25 (8) ◽  
pp. 966-969 ◽  
Author(s):  
Sükrü Beydemir ◽  
Mehmet Çiftçi ◽  
Ömer Irfan Küfrevioglu ◽  
Mehmet Emin Büyükokuroglu
Keyword(s):  
Carbonic Anhydrase ◽  
Enzyme Activities ◽  
Human Erythrocytes ◽  
Rat Erythrocytes ◽  
Gentamicin Sulfate

scholarly journals In Vitro and in Vivo Effects of Dantrolene on Carbonic Anhydrase Enzyme Activities

2004 ◽  
Vol 27 (5) ◽  
pp. 613-616 ◽  
Author(s):  
İlhami Gülçin ◽  
Şükrü Beydemir ◽  
Mehmet Emin Büyükokuroğlu
Keyword(s):  
Carbonic Anhydrase ◽  
Enzyme Activities ◽  
In Vivo Effects ◽  
Carbonic Anhydrase Enzyme

scholarly journals Protective effects of carbonic anhydrase inhibition in brain ischaemia in vitro and in vivo models

2021 ◽  
Vol 36 (1) ◽  
pp. 964-976
Author(s):  
Ilaria Dettori ◽  
Irene Fusco ◽  
Irene Bulli ◽  
Lisa Gaviano ◽  
Elisabetta Coppi ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Protective Effects ◽  
Brain Ischaemia ◽  
In Vivo Models ◽  
Carbonic Anhydrase Inhibition

scholarly journals Surface-Engineered Dendrimeric Nanoconjugates for Macrophage-Targeted Delivery of Amphotericin B: Formulation Development andIn VitroandIn VivoEvaluation

2015 ◽  
Vol 59 (5) ◽  
pp. 2479-2487 ◽  
Author(s):  
Keerti Jain ◽  
Ashwni Kumar Verma ◽  
Prabhat Ranjan Mishra ◽  
Narendra Kumar Jain
Keyword(s):  
Drug Release ◽  
Amphotericin B ◽  
Human Erythrocytes ◽  
Antileishmanial Activity ◽  
Cell Uptake ◽  
Antiparasitic Activity ◽  
Content Type ◽  
Drug Localization

ABSTRACTThe present study aimed to develop an optimized dendrimeric delivery system for amphotericin B (AmB). Fifth-generation (5.0G) poly(propylene imine) (PPI) dendrimers were synthesized, conjugated with mannose, and characterized by use of various analytical techniques, including Fourier transform infrared spectroscopy (FTIR),1H nuclear magnetic resonance (1H-NMR) spectroscopic analysis, and atomic force microscopy (AFM). Mannose-conjugated 5.0G PPI (MPPI) dendrimers were loaded with AmB and evaluated for drug loading efficiency,in vitrodrug release profile, stability, hemolytic toxicity to human erythrocytes, cytotoxicity to and cell uptake by J774A.1 macrophage cells, antiparasitic activity against intracellularLeishmania donovaniamastigotes,in vivopharmacokinetic and biodistribution profiles, drug localization index, toxicity, and antileishmanial activity. AFM showed the nanometric size of the MPPI dendrimers, with a nearly globular architecture. The conjugate showed a good entrapment efficiency for AmB, along with pH-sensitive drug release. Highly significant reductions in toxicity toward human erythrocytes and macrophage cells, without compromising the antiparasitic activity of AmB, were observed. The dendrimeric formulation of AmB showed a significant enhancement of the parasiticidal activity of AmB toward intramacrophagicL. donovaniamastigotes. In thein vitrocell uptake studies, the formulation showed selectivity toward macrophages, with significant intracellular uptake. Further pharmacokinetic and organ distribution studies elucidated the controlled delivery behavior of the formulation. The drug localization index was found to increase significantly in macrophage-rich organs.In vivostudies showed a biocompatible behavior of MPPIA, with negligible toxicity even at higher doses, and promising antileishmanial activity. From the results, we concluded that surface-engineered dendrimers may serve as optimized delivery vehicles for AmB with enhanced activity and low or negligible toxicity.

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