Nitric Oxide- and Oxygen-Derived Free Radical Generation from Control and Lipopolysaccharide-Treated Rat Polymorphonuclear Leukocyte

Nitric Oxide ◽  
2001 ◽  
Vol 5 (5) ◽  
pp. 482-493 ◽  
Author(s):  
Sonia Sethi ◽  
Prashant Sharma ◽  
Madhu Dikshit
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Polymorphonuclear Leukocyte ◽  
Free Radical Generation ◽  
Radical Generation

Nitric Oxide–Mediated Augmentation of Polymorphonuclear Free Radical Generation After Hypoxia-Reoxygenation

Blood ◽  
1999 ◽  
Vol 93 (1) ◽  
pp. 333-340
Author(s):  
Sonia Sethi ◽  
Mahendra Pratap Singh ◽  
Madhu Dikshit
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Intracellular Calcium ◽  
Free Radical Generation ◽  
Adp Ribosylation ◽  
Low Concentrations ◽  
Significant Augmentation ◽  
Radical Generation ◽  
Synthase Inhibitor ◽  
Hypoxia Reoxygenation

Polymorphonuclear leukocytes (PMNLs), nitric oxide (NO), calcium, and free radicals play an important role in hypoxia/ischemia and reoxygenation injury. In the present study, NO donors, sodium nitroprusside (SNP), and diethylamine-NO (DEA-NO) at low concentrations (10 and 100 nmol/L) potentiated, while higher (10 μmol/L to 10 mmol/L) concentrations inhibited free radical generation response in the rat PMNLs. Free radical generation response was found to be significantly augmented when hypoxic PMNLs were reoxygenated (hypoxia-reoxygenation [H-R]). This increase in free radical generation after reoxygenation or SNP (10 nmol/L) was blocked in the absence of extracellular calcium. SNP (10 nmol/L) or H-R–mediated increases in the free radical generation were prevented by the pretreatment of PMNLs with NO scavenger (hemoglobin), the polyadenine diphosphate (ADP)-ribosylation synthase inhibitor (benzamide) or the calcium channel antagonist (felodipine). A significant augmentation in the nitrite and intracellular calcium levels was observed during hypoxia. Hemoglobin pretreatment also blocked the increase in intracellular calcium levels due to SNP (10 nmol/L) or hypoxia. Thus, increased availability of NO during SNP treatment or H-R, may have led to an ADP-ribosylation–mediated increase in intracellular calcium, thereby increasing the free radical generation from the rat PMNLs.

Cardiac arrest induces decrease of nitric oxide synthase activity and increase of free radical generation in rat brain regions

1996 ◽  
Vol 220 (3) ◽  
pp. 147-150 ◽  
Author(s):  
Natalia V. Gulyaeva ◽  
Mikhail Yu. Stepanichev ◽  
Mikhail V. Onufriev ◽  
Natalia A. Lazareva ◽  
Yuri V. Zarzhetsky ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cardiac Arrest ◽  
Free Radical ◽  
Nitric Oxide Synthase ◽  
Rat Brain ◽  
Brain Regions ◽  
Free Radical Generation ◽  
Radical Generation ◽  
Oxide Synthase ◽  
Nitric Oxide Synthase Activity

scholarly journals Nitric oxide dependent increase in free radical generation mediates release of extracellular traps from human neutrophils

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Anupam Jyoti ◽  
Ravi Shankar Keshari ◽  
Sachin Kumar ◽  
Satyananda Patel ◽  
Bangalore Suresh Srinag ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Human Neutrophils ◽  
Free Radical Generation ◽  
Extracellular Traps ◽  
Radical Generation

scholarly journals NITRIC OXIDE SYNTHASE INHIBITION ON FREE RADICAL GENERATION DURING CEREBRAL HYPOXIA IN NEWBORN PIGLETS. ▴ 1389

1996 ◽  
Vol 39 ◽  
pp. 234-234 ◽  
Author(s):  
Yoshihiro Numagami ◽  
Alan B Zubrow ◽  
Anli Zhu ◽  
Om P Mishra ◽  
Maria Delivoria-Papadopoulos
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Nitric Oxide Synthase ◽  
Cerebral Hypoxia ◽  
Free Radical Generation ◽  
Newborn Piglets ◽  
Radical Generation ◽  
Oxide Synthase
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