In Vivo and in Vitro Effects of Lysine Clonixinate on Nitric Oxide Synthase in LPS-Treated and Untreated Rat Lung Preparations

Nitric Oxide ◽  
2001 ◽  
Vol 5 (2) ◽  
pp. 150-157 ◽  
Author(s):  
A.M. Franchi ◽  
G. Di Girolamo ◽  
M. Farina ◽  
A.R. de los Santos ◽  
M.L. Martı́ ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Rat Lung ◽  
Lysine Clonixinate ◽  
In Vitro Effects ◽  
Oxide Synthase

Ethanol suppresses LPS-induced mRNA for nitric oxide synthase II in alveolar macrophages in vivo and in vitro

Alcohol ◽  
1994 ◽  
Vol 11 (6) ◽  
pp. 539-547 ◽  
Author(s):  
Stanley S. Greenberg ◽  
Jianming Xie ◽  
Ye Wang ◽  
Jay Kolls ◽  
Tadeus Malinski ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Alveolar Macrophages ◽  
Oxide Synthase

scholarly journals Induction of inducible nitric oxide synthase (iNOS) in vitro and in vivo brain microgllia

1996 ◽  
Vol 108 (supplement) ◽  
pp. 115-120
Author(s):  
Yoshihisa KITAMURA ◽  
Hideaki TAKAHASHI ◽  
Yasuji MATSUOKA ◽  
Yasuyuki NOMURA ◽  
Takashi TANIGUCHI
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals Can endothelial hemoglobin-α regulate nitric oxide vasodilatory signaling?

2017 ◽  
Vol 312 (4) ◽  
pp. H854-H866 ◽  
Author(s):  
Jaimit Parikh ◽  
Adam Kapela ◽  
Nikolaos M. Tsoukias
Keyword(s):  
Mathematical Modeling ◽  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Endothelial Nitric Oxide Synthase ◽  
Cellular Models ◽  
No Signaling ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

We used mathematical modeling to investigate nitric oxide (NO)-dependent vasodilatory signaling in the arteriolar wall. Detailed continuum cellular models of calcium (Ca2+) dynamics and membrane electrophysiology in smooth muscle and endothelial cells (EC) were coupled with models of NO signaling and biotransport in an arteriole. We used this theoretical approach to examine the role of endothelial hemoglobin-α (Hbα) as a modulator of NO-mediated myoendothelial feedback, as previously suggested in Straub et al. ( Nature 491: 473–477, 2012). The model considers enriched expression of inositol 1,4,5-triphosphate receptors (IP3Rs), endothelial nitric oxide synthase (eNOS) enzyme, Ca2+-activated potassium (KCa) channels and Hbα in myoendothelial projections (MPs) between the two cell layers. The model suggests that NO-mediated myoendothelial feedback is plausible if a significant percentage of eNOS is localized within or near the myoendothelial projection. Model results show that the ability of Hbα to regulate the myoendothelial feedback is conditional to its colocalization with eNOS near MPs at concentrations in the high nanomolar range (>0.2 μM or 24,000 molecules). Simulations also show that the effect of Hbα observed in in vitro experimental studies may overestimate its contribution in vivo, in the presence of blood perfusion. Thus, additional experimentation is required to quantify the presence and spatial distribution of Hbα in the EC, as well as to test that the strong effect of Hbα on NO signaling seen in vitro, translates also into a physiologically relevant response in vivo. NEW & NOTEWORTHY Mathematical modeling shows that although regulation of nitric oxide signaling by hemoglobin-α (Hbα) is plausible, it is conditional to its presence in significant concentrations colocalized with endothelial nitric oxide synthase in myoendothelial projections. Additional experimentation is required to test that the strong effect of Hbα seen in vitro translates into a physiologically relevant response in vivo

Brain mitochondrial nitric oxide synthase: in vitro and in vivo inhibition by chlorpromazine

2004 ◽  
Vol 430 (2) ◽  
pp. 170-177 ◽  
Author(s):  
Silvia Lores-Arnaiz ◽  
Gabriela D’Amico ◽  
Analía Czerniczyniec ◽  
Juanita Bustamante ◽  
Alberto Boveris
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Mitochondrial Nitric Oxide Synthase ◽  
Oxide Synthase

scholarly journals virB-Mediated Survival of Brucella abortus in Mice and Macrophages Is Independent of a Functional Inducible Nitric Oxide Synthase or NADPH Oxidase in Macrophages

2002 ◽  
Vol 70 (9) ◽  
pp. 4826-4832 ◽  
Author(s):  
Yao-Hui Sun ◽  
Andreas B. den Hartigh ◽  
Renato de Lima Santos ◽  
L. Garry Adams ◽  
Renée M. Tsolis
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Nadph Oxidase ◽  
Inducible Nitric Oxide Synthase ◽  
Brucella Abortus ◽  
In Vivo Models ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

ABSTRACT The Brucella abortus virB locus is required for establishing chronic infection in the mouse. Using in vitro and in vivo models, we investigated whether virB is involved in evasion of the bactericidal activity of NADPH oxidase and the inducible nitric oxide synthase (iNOS) in macrophages. Elimination of NADPH oxidase or iNOS activity in macrophages in vitro increased recovery of wild-type B. abortus but not recovery of a virB mutant. In mice lacking either NADPH oxidase or iNOS, however, B. abortus infected and persisted to the same extent as it did in congenic C57BL/6 mice up until 60 days postinfection, suggesting that these host defense mechanisms are not critical for limiting bacterial growth in the mouse. A virB mutant did not exhibit increased survival in either of the knockout mouse strains, indicating that this locus does not contribute to evasion of nitrosative or oxidative killing mechanisms in vivo.

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