Serum Activation Unmasks Unique Sensitivities to Adhesion Molecule Expression in Human Coronary Artery and Heart Microvascular Endothelial Cells

2000 ◽  
Vol 59 (3) ◽  
pp. 338-344
Author(s):  
Mark W. Farrar ◽  
Rhoda McDouall ◽  
Shabeena Khan ◽  
Magdi H. Yacoub ◽  
Sean P. Allen
Keyword(s):  
Endothelial Cells ◽  
Coronary Artery ◽  
Adhesion Molecule ◽  
Microvascular Endothelial Cells ◽  
Adhesion Molecule Expression ◽  
Human Coronary Artery ◽  
Microvascular Endothelial

Methylene blue modulates adhesion molecule expression on microvascular endothelial cells

2014 ◽  
Vol 63 (8) ◽  
pp. 649-656 ◽  
Author(s):  
Isabella Werner ◽  
Fengwei Guo ◽  
Ulrich A. Stock ◽  
Michèle Lupinski ◽  
Patrick Meybohm ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Endothelial Cells ◽  
Adhesion Molecule ◽  
Microvascular Endothelial Cells ◽  
Adhesion Molecule Expression ◽  
Microvascular Endothelial

scholarly journals Small Interfering RNA Efficiently Suppresses Adhesion Molecule Expression on Pulmonary Microvascular Endothelium

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Tobias Walker ◽  
Julian Siegel ◽  
Andrea Nolte ◽  
Silke Hartmann ◽  
Angela Kornberger ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Adhesion Molecule ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Microvascular Endothelial Cells ◽  
Adhesion Molecule Expression ◽  
Small Interfering Rnas ◽  
Sirna Transfection ◽  
Microvascular Endothelium ◽  
Microvascular Endothelial

Background. Adhesion molecules are known to influence postoperative organ function, they are hardly involved in the inflammatory response following the ischemia-reperfusion injury. We sought to investigate the potency of small interfering RNAs to suppress adhesion molecule expression in human pulmonary microvascular endothelial cells.Methods. Human lung microvascular endothelial cells were transfected with specific siRNA followed by a stimulation of the cells with an inflammatory cytokine. Adhesion molecule expression was determined by FACS-analysis, and reduction of intracellular mRNA was determined by qRT-PCR. Furthermore, the attachment of isolated neutrophils on the endothelial layer was determined after siRNA transfection.Results. In summary, siRNA transfection significantly decreased the percentage positive cells in a single cocktail transfection of each adhesion molecule investigated. Adhering neutrophils were diminished as well.Conclusion. siRNA might be a promising tool for the effective suppression of adhesion molecule expression on pulmonary microvascular cells, potentially minimizing leukocyte-endothelial depending interactions of a pulmonary allograft.

Sign in / Sign up

Export Citation Format

Share Document