Estimating Nucleotide Diversity From Random Amplified Polymorphic DNA and Amplified Fragment Length Polymorphism Data

2001 ◽  
Vol 18 (1) ◽  
pp. 143-148 ◽  
Author(s):  
Richard L. Borowsky
Keyword(s):  
Length Polymorphism ◽  
Fragment Length ◽  
Nucleotide Diversity ◽  
Fragment Length Polymorphism ◽  
Random Amplified Polymorphic Dna ◽  
Amplified Fragment Length Polymorphism ◽  
Polymorphism Data

Ommatissus lybicus (Hemiptera: Tropiduchidae), an economically important pest of date palm (Arecaceae) with highly divergent populations

2018 ◽  
Vol 150 (3) ◽  
pp. 378-392 ◽  
Author(s):  
Abdoolnabi Bagheri ◽  
Yaghoub Fathipour ◽  
Majeed Askari-Seyahooei ◽  
Mehrshad Zeinalabedini
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Date Palm ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
28S Rrna ◽  
Polymorphism Data ◽  
Coi Sequences

AbstractOmmatissus lybicus de Bergevin (Hemiptera: Tropiduchidae) is a key pest of date palm (Phoenix dactylifera Linnaeus; Arecaceae) with worldwide distribution and various management strategies. To study genetic diversity of date palm hopper, a series of experiments was conducted on genetic structure and genetic diversity of 15 geographic populations of O. lybicus (Abu Musa, Bam, Bushehr, Behbahan, Tezerj, Fin, Jiroft, Shahdad, Jahrom, Ghire Karzin, Ghasre Shirin, Iran; Pakistan; Oman; Egypt; and Tunisia) by amplified fragment length polymorphism, cytochrome c oxidase subunit I (COI), and 28S rRNA markers. Analysis of molecular variance analysis of amplified fragment length polymorphism data and COI sequences revealed a significant variation among O. lybicus populations (94.12% and 65.08% similarities for amplified fragment length polymorphism and COI, respectively). The 28S rDNA sequences from different populations were identical. Phylogenetic network inferred from amplified fragment length polymorphism data and COI sequences grouped two geographically close populations (Tezerj and Bam) in the two distinct clades while far apart geographical populations bunched in the same or close clades. These two populations experience repeated exposure to heavy pesticide applications annually. In conclusion, study of the genetic structure revealed a considerable variation between O. lybicus populations under intensive chemical strategies.

Amplified fragment length polymorphism in Elymus elymoides, Elymus multisetus, and other Elymus taxa

2003 ◽  
Vol 81 (8) ◽  
pp. 789-804 ◽  
Author(s):  
Steven R Larson ◽  
Thomas A Jones ◽  
Carrie L McCracken ◽  
Kevin B Jensen
Keyword(s):  
Length Polymorphism ◽  
North American ◽  
Fragment Length ◽  
Nucleotide Diversity ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Elymus Elymoides ◽  
Genetic Groups ◽  
Nucleotide Divergence ◽  
Elymus Multisetus

The geographic and phylogenetic significance of amplified fragment length polymorphism within and among 22 Elymus elymoides (Raf.) Swezey subsp. elymoides, 24 E. elymoides subsp. brevifolius (J.G. Sm.) Barkworth, and 13 Elymus multisetus (J.G. Sm.) Burtt-Davy squirreltail accessions was assessed relative to six other North American and three Eurasian Elymus taxa. Elymus elymoides and E. multisetus, comprising Elymus sect. Sitanion (Raf.) Á. Löve, were both monophyletic and closely related compared with other congeners. The monophyly of subsp. elymoides was also supported; subsp. brevifolius, however, was paraphyletic and separated into four genetically distinct groups. Estimates of nucleotide divergence among the five E. elymoides groups range from 0.0194 to 0.0288, with approximately 0.0329 differences per site between E. elymoides and E. multisetus. Corresponding estimates of nucleotide divergence range from 0.0243 to 0.0387 among North American taxa and from 0.0337 to 0.0455 between North American and Eurasian taxa. DNA polymorphism among E. elymoides accessions was correlated with geographic provenance and previously reported quantitative traits. Distinct genetic groups of E. elymoides generally correspond to different geographic regions, whereas divergent E. multisetus and E. elymoides accessions are sympatric. Thus, taxonomic ranks of E. multisetus and E. elymoides were supported and geographic groups within E. elymoides were distinguished.Key words: AFLP, Elymus, nucleotide diversity, squirreltail.

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