Isolation and characterization of a hemin-binding cell envelope protein fromPorphyromonas gingivalis

1996 ◽  
Vol 21 (1) ◽  
pp. 65-70 ◽  
Author(s):  
Sung-Jo Kim ◽  
L. Chu ◽  
S.C. Holt
Keyword(s):  
Envelope Protein ◽  
Cell Envelope ◽  
Isolation And Characterization ◽  
Binding Cell

Isolation and characterization of a 53 kDa major cell envelope protein antigen from Treponema denticola ATCC 35405

1994 ◽  
Vol 29 (1) ◽  
pp. 70-78 ◽  
Author(s):  
S. Kokeguchi ◽  
M. Miyamoto ◽  
K. Kato ◽  
I. Tanimoto ◽  
H. Kurihara ◽  
...  
Keyword(s):  
Envelope Protein ◽  
Cell Envelope ◽  
Protein Antigen ◽  
Treponema Denticola ◽  
Isolation And Characterization

Isolation and characterization of a Clostridium sp. with cinnamoyl esterase activity and unusual cell envelope ultrastructure

1999 ◽  
Vol 172 (3) ◽  
pp. 139-149 ◽  
Author(s):  
C. S. McSweeney ◽  
Amanda Dulieu ◽  
Richard I. Webb ◽  
Therese Del Dot ◽  
Linda L. Blackall
Keyword(s):  
Esterase Activity ◽  
Cell Envelope ◽  
Isolation And Characterization ◽  
Cinnamoyl Esterase

Characterization of Cell Envelope Protein Patterns of Crop Yield Increasing Root-Colonizing Pseudomonas spp

1986 ◽  
pp. 283-287 ◽  
Author(s):  
L. de Weger ◽  
R. van Boxtel ◽  
B. van der Burg ◽  
R. Gruters ◽  
P. Geels ◽  
...  
Keyword(s):  
Crop Yield ◽  
Envelope Protein ◽  
Cell Envelope ◽  
Protein Patterns ◽  
Pseudomonas Spp

The structure of the extracellular teichoic acids from the allergy-protective bacterium Lactococcus lactis G121

2012 ◽  
Vol 393 (8) ◽  
pp. 749-755
Author(s):  
Kathleen Fischer ◽  
Evgueny Vinogradov ◽  
Buko Lindner ◽  
Holger Heine ◽  
Otto Holst
Keyword(s):  
Lactococcus Lactis ◽  
Molecular Mechanisms ◽  
Cell Envelope ◽  
Teichoic Acid ◽  
Size Exclusion ◽  
Resonance Spectroscopy ◽  
Protective Properties ◽  
Isolation And Characterization ◽  
Exclusion Chromatography

Abstract The Gram-positive bacterium Lactococcus lactis G121 is a farm isolate that protects mice from ovalbumin-induced asthma. To understand the molecular mechanisms of such allergy-protective properties, the isolation and characterization of cell envelope constituents is crucial. Here, structural analyses of the extracellular teichoic acid (EC TA) from L. lactis G121 are presented. Extraction with 0.9% saline afforded a crude TA fraction. Consecutive size exclusion chromatography on Biogel P60 and P10 matrix was performed to purify the sample. Chemical component analyses, high-resolution electrospray ionization Fourier-transformed ion cyclotron mass spectrometry, and nuclear magnetic resonance spectroscopy were conducted for structural elucidation. The EC TA was a poly(glycosylglycerol phosphate) molecule with a repeating unit of -6)-[β-d-Glcp-(1→3)-][α-d-GlcpNAc-(1→4)-]α-d-GalpNAc-(1→3)-β-d-GlcpNAc-(1→2)-glycerol-(1-P-).

Isolation and characterization of a new acidophilic Thiobacillus species (T. albertis)

1983 ◽  
Vol 29 (9) ◽  
pp. 1159-1170 ◽  
Author(s):  
R. D. Bryant ◽  
K. M. McGroarty ◽  
J. W. Costerton ◽  
E. J. Laishley
Keyword(s):  
Inclusion Bodies ◽  
Cell Envelope ◽  
Ultrastructural Studies ◽  
Isolation And Characterization ◽  
Sulfur Granule ◽  
Stabilization Procedure ◽  
Membrane Bound ◽  
Sulfur Oxidizing ◽  
Ph Range

An acidophilic, rod-shaped, sulfur-oxidizing bacterium was isolated from extremely acidic soil adjacent to a sulfur stockpile at Fox Creek, Alta. This isolate was found to grow only autotrophically by oxidizing S° and reduced inorganic sulfur compounds (S2O32− and S4O62−), placing it in the genus Thiobacillus. Aerobic growth was observed over a pH range of 2.0 to 4.5 with optimum growth at 3.5 to 4.0. Analysis of its DNA revealed a mol% G + C content of 61.5. Ultrastructural studies showed that the isolate possessed a tuft of polar flagella. A glycocalyx was observed only after the antiserum stabilization procedure, which showed it extending outwards from the outer membrane of the bacterial cell envelope. Cleavage planes of this cell showed a typical Gram-negative cell wall. The cytoplasm contained two types of inclusion bodies, carboxysomes and a single large membrane-bound sulfur granule. This isolate had morphological and physiological characteristics which were not totally comparable with other known acidiophilic thiobacilli demonstrating this bacterium to be a new Thiobacillus sp. named T.albertis.

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