Human XPA and XRCC1 DNA Repair Proteins Expressed in Yeast, Saccharomyces cerevisiae

2001 ◽  
Vol 74 (3) ◽  
pp. 380-384 ◽  
Author(s):  
Elena A. Pushnova ◽  
Kirill Ostanin ◽  
Michael P. Thelen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Repair ◽  
Yeast Saccharomyces Cerevisiae ◽  
Dna Repair Proteins

scholarly journals The essential DNA polymerases delta and epsilon are involved in repair of UV-damaged DNA in the yeast Saccharomyces cerevisiae.

1999 ◽  
Vol 46 (2) ◽  
pp. 289-298 ◽  
Author(s):  
A Hałas ◽  
Z Policińska ◽  
H Baranowska ◽  
W J Jachymczyk
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Repair ◽  
Uv Irradiation ◽  
Dna Polymerases ◽  
Relative Molecular Mass ◽  
Yeast Saccharomyces Cerevisiae ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Mutant Strains ◽  
Cellular Dna

We have studied the ability of yeast DNA polymerases to carry out repair of lesions caused by UV irradiation in Saccharomyces cerevisiae. By the analysis of postirradiation relative molecular mass changes in cellular DNA of different DNA polymerases mutant strains, it was established that mutations in DNA polymerases delta and epsilon showed accumulation of single-strand breaks indicating defective repair. Mutations in other DNA polymerase genes exhibited no defects in DNA repair. Thus, the data obtained suggest that DNA polymerases delta and epsilon are both necessary for DNA replication and for repair of lesions caused by UV irradiation. The results are discussed in the light of current concepts concerning the specificity of DNA polymerases in DNA repair.

The role of PSO and SNM genes in DNA repair of the yeast Saccharomyces cerevisiae

1990 ◽  
Vol 18 (5) ◽  
pp. 387-393 ◽  
Author(s):  
Jo�o A. P. Henriques ◽  
Martin Brendel
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Repair ◽  
Yeast Saccharomyces Cerevisiae

scholarly journals DNA repair by Rad52 liquid droplets

2019 ◽  
Author(s):  
Roxanne Oshidari ◽  
Richard Huang ◽  
Maryam Medghalchi ◽  
Elizabeth Y.W. Tse ◽  
Nasser Ashgriz ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Damage ◽  
Dna Repair ◽  
Phase Separation ◽  
Liquid Phase ◽  
Nuclear Periphery ◽  
Liquid Phase Separation ◽  
Liquid Droplets ◽  
Cellular Processes ◽  
Dna Repair Proteins

Cellular processes are influenced by liquid phase separation, but its role in DNA repair is unclear. Here, we show that in Saccharomyces cerevisiae, Rad52 DNA repair proteins at different DNA damage sites assemble liquid droplets that fuse into a repair centre droplet. This larger droplet concentrates tubulin and projects short aster-like microtubule filaments, which tether the droplet to longer microtubule filaments mediating the mobilization of damaged DNA to the nuclear periphery for repair.

A DNA endonuclease isolated from yeast nuclear extract

1978 ◽  
Vol 56 (3) ◽  
pp. 181-189 ◽  
Author(s):  
Douglas W. Bryant ◽  
Robert H. Haynes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Weight ◽  
Dna Repair ◽  
Nuclear Extract ◽  
Dna Duplexes ◽  
Replicative Form ◽  
Yeast Saccharomyces Cerevisiae ◽  
Dna Endonuclease ◽  
Circular Dna ◽  
Dna Substrate

We have isolated and partially purified a DNA endonuclease from nuclei of the yeast Saccharomyces cerevisiae. Although purified on the basis of its ability to degrade denatured DNA, the enzyme can also attack native DNA. Denatured oligonucleotide products of the enzyme are sensitive to venom phosphodiesterase (EC 3.1.4.1) but not to bovine spleen phosphodiesterase (EC 3.1.4.18). The enzyme has an estimated molecular weight of 6.6–7.5 × 104, more than twice as large as the endonucleases involved in DNA repair in Escherichia coli.When analyzed on glycerol gradients, the endonuclease sedimented as a single activity against both denatured DNA and closed circular DNA duplexes. The enzyme showed a 10-fold preference for denatured over native T7 DNA substrate, and appears to produce random nicks in a supercoiled replicative form of [Formula: see text] DNA (RFI) with no discernable preference for the unpaired bases in the supercoiled duplex. The endonuclease appears to be distinct from the yeast endonucleases previously described.

A Saccharomyces cerevisiae mutant defines a new locus essential for resistance to the antitumour drug bleomycin

1996 ◽  
Vol 42 (8) ◽  
pp. 835-843 ◽  
Author(s):  
Dindial Ramotar ◽  
Jean-Yves Masson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Hydrogen Peroxide ◽  
Dna Repair ◽  
Chromosomal Translocation ◽  
Normal Growth ◽  
Fold Increase ◽  
Growth Conditions ◽  
Dna Lesions ◽  
Methyl Methanesulfonate ◽  
Yeast Saccharomyces Cerevisiae

The antitumor drug bleomycin can produce a variety of lesions in the cellular DNA by a free radical dependent mechanism. To understand how these DNA lesions are repaired, bleomycin-hypersensitive mutants were isolated from the yeast Saccharomyces cerevisiae. We report here the analysis of one mutant, DRY25, that showed extreme sensitivity to bleomycin. This mutant also exhibited hypersensitivity to hydrogen peroxide and t-butyl hydroperoxide, but showed no sensitivity to other DNA-damaging agents, including γ-rays, ultraviolet light, and methyl methanesulfonate. Subsequent analysis revealed that strain DRY25 was severely deficient in the repair of bleomycin-induced DNA lesions. Under normal growth conditions, DRY25 displayed a 3-fold increase in the frequency of chromosomal translocation that was further stimulated by 5- to 15-fold when the cells were treated with either bleomycin or hydrogen peroxide, but not by methyl methanesulfonate, as compared with the wild type. Genetic analysis indicated that the mutant defect was independent of the nucleotide excision, postreplication, or recombinational DNA-repair pathways. These data suggest that one conceivable defect of DRY25 is that it lacks a protein that protects the cell against oxidative damage to DNA. A clone that fully complemented DRY25 defect was isolated and the possible roles of the complementing gene are discussed.Key words: yeast, bleomycin, DNA repair, mutations.

A Saccharomyces cerevisiae phleomycin-sensitive mutant, phl40, is defective in the RAD6 DNA repair gene

1996 ◽  
Vol 42 (12) ◽  
pp. 1263-1266 ◽  
Author(s):  
Chuan Hua He ◽  
Jean-Yves Masson ◽  
Dindial Ramotar
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Repair ◽  
Anticancer Agent ◽  
Radiation Treatment ◽  
Dna Lesions ◽  
Exact Nature ◽  
Repair Gene ◽  
Yeast Saccharomyces Cerevisiae ◽  
Dna Repair Gene ◽  
Cellular Dna

The antibiotic bleomycin is used as an anticancer agent for treating a variety of tumours. The antitumour effect of bleomycin is related to its ability to produce lesions such as apurinic/apyrimidinic sites and single- and double-strand breaks in the cellular DNA. Phleomycin is a structurally related form of bleomycin, but it is not used as an anticancer agent. While phleomycin can also damage DNA, neither the exact nature of these DNA lesions nor the cellular process that repairs phleomycin-induced DNA lesions is known. As a first step to understand how eukaryotic cells provide resistance to phleomycin, we used the yeast Saccharomyces cerevisiae as a model system. Several phleomycin-sensitive mutants were generated following γ-radiation treatment and among these mutants, phl40 was found to be the most sensitive to phleomycin. Molecular analysis revealed that the mutant phl40 harbored a mutation in the DNA repair gene RAD6. Moreover, a functional copy of the RAD6 gene restored full phleomycin resistance to strain phl40. Our findings indicate that the RAD6 protein is essential for yeast cellular resistance to phleomycin.Key words: yeast, phleomycin, DNA repair, RAD6.

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