Molecular Cloning of the Guinea Pig Cytomegalovirus (GPCMV) Genome as an Infectious Bacterial Artificial Chromosome (BAC) in Escherichia coli

2001 ◽  
Vol 72 (1) ◽  
pp. 15-26 ◽  
Author(s):  
Alistair McGregor ◽  
Mark R. Schleiss
Keyword(s):  
Escherichia Coli ◽  
Bacterial Artificial Chromosome ◽  
Guinea Pig ◽  
Molecular Cloning ◽  
Artificial Chromosome ◽  
Guinea Pig Cytomegalovirus

scholarly journals Stabilization of a Full-Length Infectious cDNA Clone of Transmissible Gastroenteritis Coronavirus by Insertion of an Intron

2002 ◽  
Vol 76 (9) ◽  
pp. 4655-4661 ◽  
Author(s):  
José M. González ◽  
Zoltan Pénzes ◽  
Fernando Almazán ◽  
Enrique Calvo ◽  
Luis Enjuanes
Keyword(s):  
Escherichia Coli ◽  
Bacterial Artificial Chromosome ◽  
Cell Nucleus ◽  
Viral Genome ◽  
Artificial Chromosome ◽  
Full Length ◽  
Stable Plasmid ◽  
Transmissible Gastroenteritis ◽  
Plasmid Amplification ◽  
Transmissible Gastroenteritis Coronavirus

ABSTRACT The stable propagation of a full-length transmissible gastroenteritis coronavirus (TGEV) cDNA in Escherichia coli cells as a bacterial artificial chromosome has been considerably improved by the insertion of an intron to disrupt a toxic region identified in the viral genome. The viral RNA was expressed in the cell nucleus under the control of the cytomegalovirus promoter and the intron was efficiently removed during translocation of this RNA to the cytoplasm. The insertion in two different positions allowed stable plasmid amplification for at least 200 generations. Infectious TGEV was efficiently recovered from cells transfected with the modified cDNAs.

Metabolic engineering applications of the Escherichia coli bacterial artificial chromosome

2019 ◽  
Vol 305 ◽  
pp. 43-50
Author(s):  
Gustav Sjöberg ◽  
Mónica Guevara-Martínez ◽  
Antonius J.A. van Maris ◽  
Martin Gustavsson
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Bacterial Artificial Chromosome ◽  
Artificial Chromosome ◽  
Engineering Applications
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