scholarly journals Dual-Color Fluorescence Cross-Correlation Spectroscopy for Monitoring the Kinetics of Enzyme-Catalyzed Reactions

Methods ◽  
2001 ◽  
Vol 24 (2) ◽  
pp. 104-116 ◽  
Author(s):  
Markus Rarbach ◽  
Ulrich Kettling ◽  
Andre Koltermann ◽  
Manfred Eigen
Keyword(s):  
Cross Correlation ◽  
Correlation Spectroscopy ◽  
Dual Color ◽  
Catalyzed Reactions ◽  
Enzyme Catalyzed Reactions ◽  
Kinetics Of

Probing the binding kinetics of proinflammatory cytokine–antibody interactions using dual color fluorescence cross correlation spectroscopy

The Analyst ◽  
2011 ◽  
Vol 136 (10) ◽  
pp. 2111 ◽  
Author(s):  
Chia-Yan Wu ◽  
Chuan-Keng Huang ◽  
Chao-Yu Chung ◽  
I-Ping Huang ◽  
Yeukuang Hwu ◽  
...  
Keyword(s):  
Proinflammatory Cytokine ◽  
Cross Correlation ◽  
Correlation Spectroscopy ◽  
Binding Kinetics ◽  
Dual Color ◽  
Antibody Interactions ◽  
Kinetics Of

Dual-color fluorescence cross-correlation spectroscopy on a planar optofluidic chip

Lab on a Chip ◽  
2011 ◽  
Vol 11 (8) ◽  
pp. 1502 ◽  
Author(s):  
A. Chen ◽  
M. M. Eberle ◽  
E. J. Lunt ◽  
S. Liu ◽  
K. Leake ◽  
...  
Keyword(s):  
Cross Correlation ◽  
Correlation Spectroscopy ◽  
Dual Color ◽  
Optofluidic Chip

scholarly journals Thermodynamics and Kinetics of Glycolytic Reactions. Part I: Kinetic Modeling Based on Irreversible Thermodynamics and Validation by Calorimetry

2020 ◽  
Vol 21 (21) ◽  
pp. 8341
Author(s):  
Kristina Vogel ◽  
Thorsten Greinert ◽  
Monique Reichard ◽  
Christoph Held ◽  
Hauke Harms ◽  
...  
Keyword(s):  
Equilibrium State ◽  
Kinetic Models ◽  
Metabolic Pathways ◽  
Irreversible Thermodynamics ◽  
Titration Calorimetry ◽  
Metabolic Network Analysis ◽  
Arrhenius Relation ◽  
Catalyzed Reactions ◽  
Enzyme Catalyzed Reactions ◽  
Kinetics Of

In systems biology, material balances, kinetic models, and thermodynamic boundary conditions are increasingly used for metabolic network analysis. It is remarkable that the reversibility of enzyme-catalyzed reactions and the influence of cytosolic conditions are often neglected in kinetic models. In fact, enzyme-catalyzed reactions in numerous metabolic pathways such as in glycolysis are often reversible, i.e., they only proceed until an equilibrium state is reached and not until the substrate is completely consumed. Here, we propose the use of irreversible thermodynamics to describe the kinetic approximation to the equilibrium state in a consistent way with very few adjustable parameters. Using a flux-force approach allowed describing the influence of cytosolic conditions on the kinetics by only one single parameter. The approach was applied to reaction steps 2 and 9 of glycolysis (i.e., the phosphoglucose isomerase reaction from glucose 6-phosphate to fructose 6-phosphate and the enolase-catalyzed reaction from 2-phosphoglycerate to phosphoenolpyruvate and water). The temperature dependence of the kinetic parameter fulfills the Arrhenius relation and the derived activation energies are plausible. All the data obtained in this work were measured efficiently and accurately by means of isothermal titration calorimetry (ITC). The combination of calorimetric monitoring with simple flux-force relations has the potential for adequate consideration of cytosolic conditions in a simple manner.

A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy

2006 ◽  
Vol 24 (5) ◽  
pp. 577-581 ◽  
Author(s):  
Takako Kogure ◽  
Satoshi Karasawa ◽  
Toshio Araki ◽  
Kenta Saito ◽  
Masataka Kinjo ◽  
...  
Keyword(s):  
Cross Correlation ◽  
Correlation Spectroscopy ◽  
Laser Fluorescence ◽  
Stony Coral ◽  
Single Laser ◽  
Dual Color

Dual-color Fluorescence Cross Correlation Spectroscopy on an Integrated Optofluidic Chip

2010 ◽  
Author(s):  
A. Chen ◽  
M.I. Rudenko ◽  
E.J. Lunt ◽  
B.S. Phillips ◽  
A. R. Hawkins ◽  
...  
Keyword(s):  
Cross Correlation ◽  
Correlation Spectroscopy ◽  
Dual Color ◽  
Optofluidic Chip
Sign in / Sign up

Export Citation Format

Share Document