Ligand-Induced Conformational Changes in Cyclic Nucleotide Phosphodiesterases and Cyclic Nucleotide-Dependent Protein Kinases

Methods ◽  
1998 ◽  
Vol 14 (1) ◽  
pp. 81-92 ◽  
Author(s):  
Sharron H. Francis ◽  
Der-Ming Chu ◽  
Melissa K. Thomas ◽  
Alfreda Beasley ◽  
Kennard Grimes ◽  
...  
Keyword(s):  
Protein Kinases ◽  
Conformational Changes ◽  
Cyclic Nucleotide ◽  
Cyclic Nucleotide Phosphodiesterases ◽  
Dependent Protein ◽  
Nucleotide Phosphodiesterases

Dihydro- and tetrahydroisoquinolines as inhibitors of cyclic nucleotide phosphodiesterases from dog heart

1979 ◽  
Vol 28 (8) ◽  
pp. 1307-1312 ◽  
Author(s):  
Richard G. van Inwegen ◽  
Phyllis Salaman ◽  
Vassil St. Georgiev ◽  
Ira Weinryb
Keyword(s):  
Cyclic Nucleotide ◽  
Dog Heart ◽  
Cyclic Nucleotide Phosphodiesterases ◽  
Nucleotide Phosphodiesterases

scholarly journals Cyclic nucleotide-dependent protein kinases in airway smooth muscle.

1982 ◽  
Vol 257 (19) ◽  
pp. 11609-11616 ◽  
Author(s):  
T J Torphy ◽  
W B Freese ◽  
G A Rinard ◽  
L L Brunton ◽  
S E Mayer
Keyword(s):  
Smooth Muscle ◽  
Protein Kinases ◽  
Airway Smooth Muscle ◽  
Cyclic Nucleotide ◽  
Dependent Protein

scholarly journals Cyclic Nucleotide-dependent Protein Kinases

1969 ◽  
Vol 244 (23) ◽  
pp. 6395-6402 ◽  
Author(s):  
Eishichi Miyamoto ◽  
J.F. Kuo ◽  
Paul Greengard
Keyword(s):  
Protein Kinases ◽  
Cyclic Nucleotide ◽  
Dependent Protein

A Fluorescence Polarization Assay for Cyclic Nucleotide Phosphodiesterases

2002 ◽  
Vol 7 (3) ◽  
pp. 215-222 ◽  
Author(s):  
Wei Huang ◽  
Yan Zhang ◽  
J. Richard Sportsman
Keyword(s):  
Cyclic Nucleotide ◽  
Fluorescence Polarization ◽  
Drug Targets ◽  
Second Messengers ◽  
Reaction Products ◽  
Cyclic Nucleotide Phosphodiesterases ◽  
Extracellular Signals ◽  
Fluorescence Polarization Assay ◽  
Hydrolysis Of ◽  
Nucleotide Phosphodiesterases

Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of the 3′-ester bond of cyclic AMP (cAMP) and cyclic GMP (cGMP), important second messengers in the transduction of a variety of extracellular signals. There is growing interest in the study of PDEs as drug targets for novel therapeutics. We describe the development of a homogeneous fluorescence polarization assay for PDEs based on the strong binding of PDE reaction products (i.e., AMP or GMP) onto modified nanoparticles through interactions with immobilized trivalent metal cations. This assay technology (IMAP) is applicable to both cAMP- and cGMP-specific PDEs. Results of the assay in 384- and 1536-well microplates are presented.

Antiplatelet effects of KW-7, a new inhibitor of cyclic nucleotide phosphodiesterases

2004 ◽  
Vol 483 (2-3) ◽  
pp. 187-194 ◽  
Author(s):  
Chin-Chung Wu ◽  
Wei-Ya Wang ◽  
Reen-Yen Kuo ◽  
Fang-Rong Chang ◽  
Yang-Chang Wu
Keyword(s):  
Cyclic Nucleotide ◽  
Cyclic Nucleotide Phosphodiesterases ◽  
Nucleotide Phosphodiesterases
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