Chromosomal Mapping of Core Histone Acetylation by Immunoselection

Methods ◽  
1997 ◽  
Vol 12 (1) ◽  
pp. 48-56 ◽  
Author(s):  
Colyn Crane-Robinson ◽  
Tim R. Hebbes ◽  
Alison L. Clayton ◽  
Alan W. Thorne
Keyword(s):  
Histone Acetylation ◽  
Chromosomal Mapping ◽  
Core Histone

The cancer preventive peptide lunasin from wheat inhibits core histone acetylation

2007 ◽  
Vol 255 (1) ◽  
pp. 42-48 ◽  
Author(s):  
Hyung Jin Jeong ◽  
Jin Boo Jeong ◽  
Dae Seop Kim ◽  
Jae Ho Park ◽  
Jung Bok Lee ◽  
...  
Keyword(s):  
Histone Acetylation ◽  
Core Histone

Relationship between Core Histone Acetylation and Histone H10 Gene Activity

1994 ◽  
Vol 224 (3) ◽  
pp. 885-892 ◽  
Author(s):  
Valerie Girardot ◽  
Thierry Rabilloud ◽  
Minoru Yoshida ◽  
Teruhiko Beppu ◽  
Jean-Jacques Lawrence ◽  
...  
Keyword(s):  
Histone Acetylation ◽  
Gene Activity ◽  
Core Histone

scholarly journals Developmental activation of the lysozyme gene in chicken macrophage cells is linked to core histone acetylation at its enhancer elements

2006 ◽  
Vol 34 (14) ◽  
pp. 4025-4035 ◽  
Author(s):  
Fiona A. Myers ◽  
Pascal Lefevre ◽  
Evangelia Mantouvalou ◽  
Kimberley Bruce ◽  
Claire Lacroix ◽  
...  
Keyword(s):  
Histone Acetylation ◽  
Core Histone ◽  
Lysozyme Gene ◽  
Macrophage Cells ◽  
Chicken Macrophage

scholarly journals Chromosomal mapping of rRNA genes, core histone genes and telomeric sequences in Brachidontes puniceus and Brachidontes rodriguezi (Bivalvia, Mytilidae)

BMC Genetics ◽  
2010 ◽  
Vol 11 (1) ◽  
pp. 109 ◽  
Author(s):  
Concepción Pérez-García ◽  
Jorge Guerra-Varela ◽  
Paloma Morán ◽  
Juan J Pasantes
Keyword(s):  
Chromosomal Mapping ◽  
Core Histone ◽  
Rrna Genes ◽  
Histone Genes ◽  
Telomeric Sequences

Isoflavones stimulate estrogen receptor-mediated core histone acetylation

2004 ◽  
Vol 317 (1) ◽  
pp. 259-264 ◽  
Author(s):  
Tao Hong ◽  
Takeya Nakagawa ◽  
WeiJun Pan ◽  
Mi Young Kim ◽  
W Lee Kraus ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Histone Acetylation ◽  
Core Histone

scholarly journals Disruption of Higher-Order Folding by Core Histone Acetylation Dramatically Enhances Transcription of Nucleosomal Arrays by RNA Polymerase III

1998 ◽  
Vol 18 (8) ◽  
pp. 4629-4638 ◽  
Author(s):  
Christin Tse ◽  
Takashi Sera ◽  
Alan P. Wolffe ◽  
Jeffrey C. Hansen
Keyword(s):  
Rna Polymerase ◽  
Histone Acetylation ◽  
Molecular Mechanisms ◽  
Chromatin Condensation ◽  
Rna Polymerase Iii ◽  
Higher Order ◽  
Core Histone ◽  
Rrna Gene ◽  
Histone Octamer ◽  
Polymerase Iii

ABSTRACT We have examined the effects of core histone acetylation on the transcriptional activity and higher-order folding of defined 12-mer nucleosomal arrays. Purified HeLa core histone octamers containing an average of 2, 6, or 12 acetates per octamer (8, 23, or 46% maximal site occupancy, respectively) were assembled onto a DNA template consisting of 12 tandem repeats of a 208-bp Lytechinus 5S rRNA gene fragment. Reconstituted nucleosomal arrays were transcribed in a Xenopus oocyte nuclear extract and analyzed by analytical hydrodynamic and electrophoretic approaches to determine the extent of array compaction. Results indicated that in buffer containing 5 mM free Mg2+ and 50 mM KCl, high levels of acetylation (12 acetates/octamer) completely inhibited higher-order folding and concurrently led to a 15-fold enhancement of transcription by RNA polymerase III. The molecular mechanisms underlying the acetylation effects on chromatin condensation were investigated by analyzing the ability of differentially acetylated nucleosomal arrays to fold and oligomerize. In MgCl2-containing buffer the folding of 12-mer nucleosomal arrays containing an average of two or six acetates per histone octamer was indistinguishable, while a level of 12 acetates per octamer completely disrupted the ability of nucleosomal arrays to form higher-order folded structures at all ionic conditions tested. In contrast, there was a linear relationship between the extent of histone octamer acetylation and the extent of disruption of Mg2+-dependent oligomerization. These results have yielded new insight into the molecular basis of acetylation effects on both transcription and higher-order compaction of nucleosomal arrays.

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