Analysis of DNA–Protein Interactions in the Nervous System Using the Electrophoretic Mobility Shift Assay

Methods ◽  
1996 ◽  
Vol 10 (3) ◽  
pp. 301-311 ◽  
Author(s):  
Susan E. Lewis ◽  
Christine Konradi
Keyword(s):  
Nervous System ◽  
Electrophoretic Mobility Shift Assay ◽  
Electrophoretic Mobility ◽  
Protein Interactions ◽  
Mobility Shift ◽  
Mobility Shift Assay ◽  
Dna Protein Interactions

Detection of RNA‐protein interactions using a highly sensitive non‐radioactive electrophoretic mobility shift assay

2019 ◽  
Vol 40 (9) ◽  
pp. 1365-1371 ◽  
Author(s):  
Gerasimos Daras ◽  
Anastasios Alatzas ◽  
Dikran Tsitsekian ◽  
Dimitris Templalexis ◽  
Stamatis Rigas ◽  
...  
Keyword(s):  
Electrophoretic Mobility Shift Assay ◽  
Electrophoretic Mobility ◽  
Protein Interactions ◽  
Mobility Shift ◽  
Highly Sensitive ◽  
Mobility Shift Assay

Assessment of small ligand-protein interactions by electrophoretic mobility shift assay using DNA-modified ligand as a sensing probe

2007 ◽  
Vol 29 (5) ◽  
pp. 785-789 ◽  
Author(s):  
Hisakage Funabashi ◽  
Michito Ubukata ◽  
Takashi Ebihara ◽  
Masuo Aizawa ◽  
Masayasu Mie ◽  
...  
Keyword(s):  
Electrophoretic Mobility Shift Assay ◽  
Electrophoretic Mobility ◽  
Protein Interactions ◽  
Mobility Shift ◽  
Mobility Shift Assay

A fluorescence based non-radioactive electrophoretic mobility shift assay

2000 ◽  
Vol 78 (2) ◽  
pp. 163-170 ◽  
Author(s):  
K Ruscher ◽  
M Reuter ◽  
D Kupper ◽  
G Trendelenburg ◽  
U Dirnagl ◽  
...  
Keyword(s):  
Electrophoretic Mobility Shift Assay ◽  
Electrophoretic Mobility ◽  
Mobility Shift ◽  
Mobility Shift Assay

scholarly journals Human replication-dependent histone H3 genes are activated by a tandemly arranged pair of two CCAAT boxes

2004 ◽  
Vol 384 (2) ◽  
pp. 317-326 ◽  
Author(s):  
Heiner KOESSLER ◽  
Joerg KAHLE ◽  
Christa BODE ◽  
Detlef DOENECKE ◽  
Werner ALBIG
Keyword(s):  
Electrophoretic Mobility Shift Assay ◽  
Electrophoretic Mobility ◽  
Histone H3 ◽  
Maximal Activity ◽  
Significant Loss ◽  
Tata Box ◽  
Mobility Shift ◽  
Nuclear Factor Y ◽  
Binding Factor ◽  
Mobility Shift Assay

We have analysed the transcriptional regulation of the human histone H3 genes using promoter deletion series, scanning mutagenesis, specific mutagenesis and electrophoretic mobility-shift assay experiments. The promoters of five of the six examined histone H3 genes showed near-maximal activity at lengths of 133–227 bp: H3/d 198 bp, H3/h 147 bp, H3/k 133 bp, H3/m 227 bp, H3/n 140 bp (exception H3/i). To search for functional cis-elements within these regions, we performed scanning mutagenesis of the two histone H3 promoters H3/k and H3/m. Mutagenesis revealed that the functional framework of the histone H3 promoters consists of a TATA box and two tandemly arranged CCAAT boxes in relatively fixed positions. Alterations of the distance between the CCAAT boxes and of the distance between the CCAAT boxes and the TATA box resulted in significant loss of activity. In electrophoretic mobility-shift assay experiments, the factor CBF (CCAAT-binding factor)/NF-Y (nuclear factor-Y) bound to isolated CCAAT boxes of the H3/k promoter. This suggests that an initiation complex is formed on the histone H3 promoter that has a defined structure and limited flexibility, consisting of two molecules of CBF/NF-Y and further (general or specific) transcription factors.

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