Rapid, sensitive, microbial detection by gene amplification using restriction endonuclease target sequences

Louise A. Metherell; Carolyn Hurst; Ian J. Bruce

doi:10.1006/mcpr.1997.0120

Rapid, sensitive, microbial detection by gene amplification using restriction endonuclease target sequences

Molecular and Cellular Probes ◽

10.1006/mcpr.1997.0120 ◽

1997 ◽

Vol 11 (4) ◽

pp. 297-308 ◽

Cited By ~ 1

Author(s):

Louise A. Metherell ◽

Carolyn Hurst ◽

Ian J. Bruce

Keyword(s):

Restriction Endonuclease ◽

Gene Amplification ◽

Microbial Detection ◽

Target Sequences

Download Full-text

TaqI digestion reveals fractions of satellite DNAs on human chromosomes

Genome ◽

10.1139/g91-039 ◽

1991 ◽

Vol 34 (2) ◽

pp. 251-254 ◽

Cited By ~ 6

Author(s):

I. Tagarro ◽

J. J. González-Aguilera ◽

A. Ma Fernández-Peralta

Keyword(s):

Restriction Endonuclease ◽

Human Chromosomes ◽

Experimental Conditions ◽

Satellite Dnas ◽

Final Reaction ◽

Dna Loss ◽

Target Sequences ◽

Main Chromosome ◽

Taqi Restriction Endonuclease

Restriction endonuclease TaqI has been known as a nonbanding restriction endonuclease when it is used on fixed human chromosomes. However, a specific TaqI digestion can be obtained after varying experimental conditions such as concentration of enzyme, time of incubation, and volume of the final reaction mixture. This digestion consists of an extensive DNA loss in heterochromatin subregions of chromosomes 1, 9, 15, 16, and Y. These regions essentially coincide with those corresponding to the main chromosome locations of satellite II DNA, whose tandem repeated units contain many TaqI target sequences, and some satellite III DNA domains enriched in TaqI sites.Key words: TaqI restriction endonuclease, heterochromatin, satellite DNAs, human chromosomes.

Download Full-text

Binding and Recognition of GATATC Target Sequences by theEcoRV Restriction Endonuclease: A Study Using Fluorescent Oligonucleotides and Fluorescence Polarization†

Biochemistry ◽

10.1021/bi001956p ◽

2001 ◽

Vol 40 (8) ◽

pp. 2484-2494 ◽

Cited By ~ 47

Author(s):

Sarah L. Reid ◽

Damian Parry ◽

Hsiao-Hui Liu ◽

Bernard A. Connolly

Keyword(s):

Restriction Endonuclease ◽

Fluorescence Polarization ◽

Target Sequences

Download Full-text

Genotyping of apolipoprotein A-IV by digestion of amplified DNA with restriction endonuclease Fnu4HI: use of a tailored primer to abolish additional recognition sites during the gene amplification

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)42078-4 ◽

1991 ◽

Vol 32 (3) ◽

pp. 545-549

Author(s):

H Tenkanen

Keyword(s):

Restriction Endonuclease ◽

Gene Amplification ◽

Recognition Sites ◽

Apolipoprotein A

Download Full-text

Detection of three separate DNA polymorphisms in the human lipoprotein lipase gene by gene amplification and restriction endonuclease digestion.

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)41422-1 ◽

1992 ◽

Vol 33 (7) ◽

pp. 1067-1072

Author(s):

T Gotoda ◽

N Yamada ◽

T Murase ◽

H Shimano ◽

M Shimada ◽

...

Keyword(s):

Restriction Endonuclease ◽

Lipoprotein Lipase ◽

Gene Amplification ◽

Dna Polymorphisms ◽

Restriction Endonuclease Digestion ◽

Lipase Gene ◽

Lipoprotein Lipase Gene ◽

Endonuclease Digestion

Download Full-text

Electron microscopic assays of restriction endonuclease cutting, exonuclease digestion, and hybridization

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100113317 ◽

1984 ◽

Vol 42 ◽

pp. 686-687

Author(s):

Mark Hannibal ◽

Jacob Varkey ◽

Michael Beer

Keyword(s):

Electron Microscope ◽

Low Temperature ◽

Restriction Endonuclease ◽

Double Helix ◽

Test Material ◽

Electron Microscopic ◽

Exonuclease Iii ◽

Dna Molecule ◽

Linear Molecules ◽

Exonuclease Digestion

Workman and Langmore have recently proposed a procedure for isolating particular chromatin fragments. The method requires restriction endonuclease cutting of the chromatin and a probe, their digestion with two exonucleases which leave complimentary single strand termini and low temperature hybridization of these. We here report simple electron microscopic monitoring of the four reactions involved.Our test material was ϕX-174 RF DNA which is cut once by restriction endonuclease Xho I. The conversion of circles to linear molecules was followed in Kleinschmidt spreads. Plate I shows a circular and a linear DNA molecule. The rate of cutting is shown in Figure 1.After completion of the endonuclease cutting, one portion of the DNA was treated with exonuclease III, an enzyme known to digest the 3' terminals of double helical DNA. Aliquots when examined in the electron microscope reveal a decreasing length of double helix and increasing bushes at the ends.

Download Full-text

Use of Antibody to aid Visualization of Protein at the Termini of Bacteriophage Ø29 DNA

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600002385 ◽

1980 ◽

Vol 38 ◽

pp. 540-541

Author(s):

Dwight Anderson ◽

Charlene Peterson ◽

Gursaran Notani ◽

Bernard Reilly

Keyword(s):

Electron Microscopy ◽

Bacillus Subtilis ◽

Dna Synthesis ◽

Restriction Endonuclease ◽

Protein Complex ◽

Protein Product ◽

Viral Dna ◽

Small Proteins ◽

Antibody Labeling ◽

Subtilis Bacteriophage

The protein product of cistron 3 of Bacillus subtilis bacteriophage Ø29 is essential for viral DNA synthesis and is covalently bound to the 5’-termini of the Ø29 DNA. When the DNA-protein complex is cleaved with a restriction endonuclease, the protein is bound to the two terminal fragments. The 28,000 dalton protein can be visualized by electron microscopy as a small dot and often is seen only when two ends are in apposition as in multimers or in glutaraldehyde-fixed aggregates. We sought to improve the visibility of these small proteins by use of antibody labeling.

Download Full-text