Urinary inhibitors of polymerase chain reaction and ligase chain reaction and testing of multiple specimens may contribute to lower assay sensitivities for diagnosingChlamydia trachomatisinfected women

1997 ◽  
Vol 11 (4) ◽  
pp. 243-249 ◽  
Author(s):  
Max A. Chernesky ◽  
Dan Jang ◽  
John Sellors ◽  
Kathy Luinstra ◽  
Sylvia Chong ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Urinary Inhibitors ◽  
Polymerase Chain

scholarly journals A Comparison of Ligase Chain Reaction to Polymerase Chain Reaction in the Detection ofChlamydia trachomatisEndocervical Infections

1998 ◽  
Vol 6 (2) ◽  
pp. 57-60 ◽  
Author(s):  
J. D. Davis ◽  
P. K. Riley ◽  
C. W. Peters ◽  
K. H. Rand
Keyword(s):  
Polymerase Chain Reaction ◽  
Positive Predictive Value ◽  
Negative Predictive Value ◽  
Predictive Value ◽  
Gold Standard ◽  
Chain Reaction ◽  
True Negative ◽  
Predictive Values ◽  
Ligase Chain Reaction ◽  
Polymerase Chain

Objective:To compare the reliability of ligase chain reaction (LCR) to polymerase chain reaction (PCR) in detectingChlamydia trachomatisendocervical infections.Methods:We conducted a prospective study of 486 patients at risk for chlamydial infection of the endocervix. We obtained two endocervical specimens from each patient and used LCR and PCR to detectC. trachomatis. Discrepant results between the two techniques were resolved by repeat testing and by testing for the major outer membrane protein (MOMP) gene, if necessary. We determined the sensitivity, specificity, positive predictive value, and negative predictive value for each test, using concordant results or MOMP gene results as the “gold standard”.Results:Of the 486 patients, 42 (8.6%) had evidence ofC. trachomatisinfection after resolution of discrepant results. Of the 42 true positive specimens, 41 were positive by initial LCR and 38 were positive by initial PCR. Of the 444 true negative specimens, none had a positive initial LCR result, while 2 had a positive initial PCR test. Therefore, compared to the gold standard, LCR had a sensitivity of 97.6% and specificity of 100%, while PCR had a sensitivity of 90% and a specificity of 99.5%. The positive and negative predictive values of LCR were 100% and 99.8%, respectively. PCR had a positive predictive value of 95% and a negative predictive value of 99.1%. The difference in sensitivity of LCR versus PCR was not statistically significant (P= .125).Conclusion:LCR and PCR perform equally well in detectingC. trachomatisendocervical infections.

Detection of K-rasOncogene Mutations by Polymerase Chain Reaction-Based Ligase Chain Reaction

1996 ◽  
Vol 239 (2) ◽  
pp. 153-159 ◽  
Author(s):  
Teresa A. Lehman ◽  
Frank Scott ◽  
Michael Seddon ◽  
Karen Kelly ◽  
Edward C. Dempsey ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Polymerase Chain
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