A Crucial Role of Caspase 3 and Caspase 8 in Paclitaxel-Induced Apoptosis

1999 ◽  
Vol 2 (1) ◽  
pp. 36-41 ◽  
Author(s):  
Haruki Oyaizu ◽  
Yasushi Adachi ◽  
Shigeru Taketani ◽  
Rikio Tokunaga ◽  
Shirou Fukuhara ◽  
...  
Keyword(s):  
Crucial Role ◽  
Caspase 3 ◽  
Caspase 8 ◽  
Induced Apoptosis

scholarly journals Important Role of β1-Integrin in Fucoidan-Induced Apoptosis via Caspase-8 Activation

2012 ◽  
Vol 76 (6) ◽  
pp. 1163-1168 ◽  
Author(s):  
Yumi YAMASAKI ◽  
Masao YAMASAKI ◽  
Hirofumi TACHIBANA ◽  
Koji YAMADA
Keyword(s):  
Caspase 8 ◽  
Induced Apoptosis

Mechanism of cadmium induced apoptosis in human peripheral blood lymphocytes: The role of p53, Fas and Caspase-3

2013 ◽  
Vol 36 (3) ◽  
pp. 1033-1039 ◽  
Author(s):  
Abdullah H. Al-Assaf ◽  
Ali M. Alqahtani ◽  
Ali A. Alshatwi ◽  
Naveed A. Syed ◽  
Gowhar Shafi ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Caspase 3 ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Induced Apoptosis

scholarly journals Azithromycin Promotes the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells after Stimulation with TNF-α

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Tingting Meng ◽  
Ying Zhou ◽  
Jingkun Li ◽  
Meilin Hu ◽  
Xiaomeng Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Alkaline Phosphatase ◽  
Osteogenic Differentiation ◽  
Alkaline Phosphatase Activity ◽  
Periodontal Ligament ◽  
Caspase 3 ◽  
Caspase 8 ◽  
Periodontal Ligament Stem Cells ◽  
Alizarin Red ◽  
Induced Apoptosis

Background and Objective. This study investigated the effects and underlying mechanisms of azithromycin (AZM) treatment on the osteogenic differentiation of human periodontal ligament stem cells (PDLSCs) after their stimulation with TNF-α in vitro. Methods. PDLSCs were isolated from periodontal ligaments from extracted teeth, and MTS assay was used to evaluate whether AZM and TNF-α had toxic effects on PDLSCs viability and proliferation. After stimulating PDLSCs with TNF-α and AZM, we analyzed alkaline phosphatase staining, alkaline phosphatase activity, and alizarin red staining to detect osteogenic differentiation. Real-time quantitative polymerase chain reaction (RT-qPCR) analysis was performed to detect the mRNA expression of osteogenic-related genes, including RUNX2, OCN, and BSP. Western blotting was used to measure the NF-κB signaling pathway proteins p65, phosphorylated p65, IκB-α, phosphorylated IκB-α, and β-catenin as well as the apoptosis-related proteins caspase-8 and caspase-3. Annexin V assay was used to detect PDLSCs apoptosis. Results. TNF-α stimulation of PDLSCs decreased alkaline phosphatase and alizarin red staining, alkaline phosphatase activity, and mRNA expression of RUNX2, OCN, and BSP in osteogenic-conditioned medium. AZM enhanced the osteogenic differentiation of PDLSCs that were stimulated with TNF-α. Western blot analysis showed that β-catenin, phosphorated p65, and phosphorylated IκB-α protein expression decreased in PDLSCs treated with AZM. In addition, pretreatment of PDLSCs with AZM (10 μg/ml, 20 μg/ml) prevented TNF-α-induced apoptosis by decreasing caspase-8 and caspase-3 expression. Conclusions. Our results showed that AZM promotes PDLSCs osteogenic differentiation in an inflammatory microenvironment by inhibiting the WNT and NF-κB signaling pathways and by suppressing TNF-α-induced apoptosis. This suggests that AZM has potential as a clinical therapeutic for periodontitis.

scholarly journals UFL1 Alleviates LPS-Induced Apoptosis by Regulating the NF-κB Signaling Pathway in Bovine Ovarian Granulosa Cells

Biomolecules ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 260 ◽  
Author(s):  
Xinling Wang ◽  
Chengmin Li ◽  
Yiru Wang ◽  
Lian Li ◽  
Zhaoyu Han ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Granulosa Cells ◽  
Protein Concentration ◽  
Caspase 3 ◽  
Nuclear Factor Κb ◽  
Ovarian Granulosa Cells ◽  
Induced Apoptosis ◽  
Apoptosis Level ◽  
Lps Treatment

Ubiquitin-like modifier 1 ligating enzyme 1 (UFL1) is an E3 ligase of ubiquitin fold modifier 1 (UFM1), which can act together with its target protein to inhibit the apoptosis of cells. Lipopolysaccharides (LPS) can affect the ovarian health of female animals by affecting the apoptosis of ovarian granulosa cells. The physiological function of UFL1 on the apoptosis of bovine (ovarian) granulosa cells (bGCs) remains unclear; therefore, we focused on the modulating effect of UFL1 on the regulation of LPS-induced apoptosis in ovarian granulosa cells. Our study found that UFL1 was expressed in both the nucleus and cytoplasm of bGCs. The results here demonstrated that LPS caused a significant increase in the apoptosis level of bGCs in cows, and also dramatically increased the expression of UFL1. Furthermore, we found that UFL1 depletion caused a significant increase in apoptosis (increased the expression of BAX/BCL-2 and the activity of caspase-3). Conversely, the overexpression of UFL1 relieved the LPS-induced apoptosis. In order to assess whether the inhibition of bGCs apoptosis involved in the nuclear factor-κB (NF-κB) signaling pathway resulted from UFL1, we detected the expression of NF-κB p-p65. LPS treatment resulted in a significant upregulation in the protein concentration of NF-κB p-p65, and knockdown of UFL1 further increased the phosphorylation of NF-κB p65, while UFL1 overexpression significantly inhibited the expression of NF-κB p-p65. Collectively, UFL1 could suppress LPS-induced apoptosis in cow ovarian granulosa cells, likely via the NF-κB pathway. These results identify a novel role of UFL1 in the modulation of bGC apoptosis, which may be a potential signaling target to improve the reproductive health of dairy cows.

scholarly journals Inflammatory protein sPLA2-IIA abrogates TNFα-induced apoptosis in human astroglioma cells: Crucial role of ERK

2009 ◽  
Vol 1793 (12) ◽  
pp. 1837-1847 ◽  
Author(s):  
Elvira Ibeas ◽  
Lucía Fuentes ◽  
Rubén Martín ◽  
Marita Hernández ◽  
María Luisa Nieto
Keyword(s):  
Crucial Role ◽  
Inflammatory Protein ◽  
Induced Apoptosis ◽  
Astroglioma Cells

scholarly journals Differential role of caspase-8 and BID activation during radiation- and CD95-induced apoptosis

Oncogene ◽  
2000 ◽  
Vol 19 (9) ◽  
pp. 1181-1190 ◽  
Author(s):  
C Belka ◽  
J Rudner ◽  
S Wesselborg ◽  
A Stepczynska ◽  
P Marini ◽  
...  
Keyword(s):  
Caspase 8 ◽  
Induced Apoptosis

scholarly journals Mechanistic insight into WEB-2170-induced apoptosis in human acute myelogenous leukemia cells: The crucial role of PTEN

2009 ◽  
Vol 37 (10) ◽  
pp. 1176-1185.e21 ◽  
Author(s):  
Cristina Cellai ◽  
Anna Laurenzana ◽  
Elisa Bianchi ◽  
Sara Sdelci ◽  
Rossella Manfredini ◽  
...  
Keyword(s):  
Acute Myelogenous Leukemia ◽  
Crucial Role ◽  
Myelogenous Leukemia ◽  
Leukemia Cells ◽  
Mechanistic Insight ◽  
Acute Myelogenous ◽  
Induced Apoptosis ◽  
Insight Into

The role of caspase family protease, caspase-3 on cisplatin-induced apoptosis in cisplatin-resistant A431 cell line

2000 ◽  
Vol 46 (3) ◽  
pp. 241-245 ◽  
Author(s):  
Hiroshi Mese ◽  
Akira Sasaki ◽  
Shuko Nakayama ◽  
Rafael E. Alcalde ◽  
Tomohiro Matsumura
Keyword(s):  
Cell Line ◽  
Caspase 3 ◽  
A431 Cell ◽  
Caspase Family ◽  
A431 Cell Line ◽  
Induced Apoptosis
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