Molecular phylogeny and status of Diploicia and Diplotomma, with observations on Diploicia subcanescens and Diplotomma rivas-martinezii

2002 ◽  
Vol 34 (6) ◽  
pp. 509-519 ◽  
Author(s):  
María del Carmen Molina ◽  
Ana Crespo ◽  
Oscar Blanco ◽  
Néstor Hladun ◽  
David L. Hawksworth
Keyword(s):  
Molecular Phylogeny ◽  
Sequence Data ◽  
Molecular Data ◽  
Rdna Sequence ◽  
Its Rdna ◽  
New Combination ◽  
Success Rates ◽  
Species Pair ◽  
Monophyletic Clade ◽  
Rdna Sequence Data

AbstractITS rDNA sequence data shows that Diploicia and Diplotomma species form a monophyletic clade distinct from other Buellia species. This indicates that Diplotomma merits acceptance as a genus, and suggests that Diploicia should be treated as a synonym of Diplotomma, the earlier name. The data also shows Diploicia subcanescens, considered the fertile counterpart in a species pair with D. canescens, is nested within D. canescens and should be treated as a synonym despite reported chemical differences. In addition, the molecular data support the distinctness of Diplotomma rivas-martinezii, a species restricted to gypsum rocks in Spain, from the widespread D. venustum, which grows on calcareous rocks. Aposymbiotic cultures suggest that D. rivas-martinezii also differs from D. venustum in its germination and isolation success rates. One new combination is made: Diplotomnta pulverulenta (Anzi) D. Hawksw. (syn. Abrothallus pulverulentus Anzi) for the lichenicolous species previously known as Buellia pulverulenta.

A new entomopathogenic nematode, Steinernema colombiense n. sp. (Nematoda: Steinernematidae), from Colombia

Nematology ◽  
2008 ◽  
Vol 10 (4) ◽  
pp. 561-574 ◽  
Author(s):  
Juan Carlos López-Núñez ◽  
Kathryn Plichta ◽  
Carmenza E. Góngora-Botero ◽  
S. Patricia Stock
Keyword(s):  
Excretory Pore ◽  
Sequence Data ◽  
Entomopathogenic Nematode ◽  
Tail Length ◽  
Rdna Sequence ◽  
Its Rdna ◽  
Infective Juvenile ◽  
Diagnostic Features ◽  
Rdna Sequences ◽  
Rdna Sequence Data

Abstract A new entomopathogenic nematode, Steinernema colombiense n. sp., is described from Colombia. Morphological, molecular (28S and ITS rDNA sequence data) and cross-hybridisation studies were used for diagnostics and identification purposes. In addition, 28S and ITS rDNA sequence data were used to assess evolutionary relationships of the new species with other Steinernema spp. Morphological diagnostic features for S. colombiense n. sp. include morphometric features of the third-stage infective juvenile, including body length of 636 (549-732) μm, narrow body diam. (31 (22-36) μm), position of the excretory pore (35 (31-40) μm), tail length (41 (32-53) μm), D% = 29 (25-33) and E% = 205 (138-284). In addition, males of first and second generations are characterised by the morphology of the spicules and gubernaculum, the number and arrangement of the genital papillae and the excretory pore position (at 67 (56-76) and 54 (46-63) μm, for first and second generations, respectively). In addition to these traits, 28S and ITS rDNA sequences analyses both showed this species to be a distinct and unique entity.

Congruence of morphological, reproductive and ITS rDNA sequence data in some AustralasianEunotia bilunaris(Bacillariophyta)

2007 ◽  
Vol 42 (1) ◽  
pp. 61-79 ◽  
Author(s):  
Pieter Vanormelingen ◽  
Victor A. Chepurnov ◽  
David G. Mann ◽  
Sylvie Cousin ◽  
Wim Vyverman
Keyword(s):  
Sequence Data ◽  
Rdna Sequence ◽  
Its Rdna ◽  
Rdna Sequence Data

Genetic variation amongSclerotiumisolates from Benin and South Africa, determined using mycelial compatibility and ITS rDNA sequence data

2005 ◽  
Vol 34 (1) ◽  
pp. 19 ◽  
Author(s):  
Appolinaire Adandonon ◽  
Theresa A. S. Aveling ◽  
Nicolaas A. van der Merwe ◽  
Gina Sanders
Keyword(s):  
South Africa ◽  
Genetic Variation ◽  
Sequence Data ◽  
Rdna Sequence ◽  
Its Rdna ◽  
Rdna Sequence Data ◽  
Mycelial Compatibility

scholarly journals New contributions to two ciliate genera (Ciliophora, Heterotrichea) based on morphological and molecular analyses, with description of a new Gruberia species

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Yong Chi ◽  
Yuqing Li ◽  
Qianqian Zhang ◽  
Mingzhen Ma ◽  
Alan Warren ◽  
...  
Keyword(s):  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Large Body ◽  
Molecular Data ◽  
Small Subunit ◽  
Rdna Sequence ◽  
Small Subunit Rdna ◽  
Rdna Sequence Data ◽  
Molecular Information ◽  
Aquatic Food

Abstract Background Heterotrichous ciliates are common members of microeukaryote communities which play important roles in both the transfer of material and the flow of energy in aquatic food webs. This group has been known for over two centuries due to their large body size and cosmopolitan distribution. Nevertheless, species identification and phylogenetic relationships of heterotrichs remain challenging due to the lack of accurate morphological information and insufficient molecular data. Results The morphology and phylogeny of two heterotrichous ciliates, namely Gruberia foissneri spec. nov. and Linostomella vorticella (Ehrenberg, 1833) Aescht in Foissner et al., 1999, were studied using rigorous methods (living morphology, stained preparations, and small subunit rDNA sequence data). Gruberia foissneri spec. nov. is morphologically very similar to G. uninucleata Kahl, 1932, however, it can be distinguished from the latter by having more ciliary rows (about 32 vs. about 20) and macronuclear shape (sausage-shaped vs. ellipsoid). Based on a combination of previous and present studies, an improved diagnosis of L. vorticella is supplied and several taxonomic anomalies are clarified. In addition, phylogenetic analyses based on SSU rDNA sequence data support the generic assignment of these two species. Conclusions Modern ciliate taxonomy should be performed by means of detailed living observation, stained preparations and molecular information. For those species that have been reported in previous studies, it is necessary to provide as much useful information as possible using state-of-the-art methods in order to resolve taxonomic anomalies.

scholarly journals Phylogeny of two poorly known ciliate genera (Ciliophora, Heterotrichea), with notes on the redefinition of Gruberia uninucleata Kahl, 1932 and Linostomella vorticella (Ehrenberg, 1833) based on populations found in China

2020 ◽  
Author(s):  
Yong Chi ◽  
Yuqing Li ◽  
Qianqian Zhang ◽  
Mingzhen Ma ◽  
Alan Warren ◽  
...  
Keyword(s):  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Large Body ◽  
Molecular Data ◽  
Small Subunit ◽  
Ssu Rdna ◽  
Rdna Sequence ◽  
Rdna Sequence Data ◽  
Aquatic Food ◽  
First Time

Abstract Background Heterotrichous ciliates are common members of microeukaryote communities which play important roles in the transfer of material and energy flow in aquatic food webs. This group has been known over two centuries due to their large body size and cosmopolitan distribution. Nevertheless, species identification and phylogenetic relationships of heterotrichs remain challenging due to the lack of accurate morphological information and insufficient molecular data. Results The morphology and phylogeny of two poorly known heterotrichous ciliates, Gruberia uninucleata Kahl, 1932 and Linostomella vorticella (Ehrenberg, 1833) Aescht in Foissner et al. , 1999, were investigated based on their living morphology, infraciliature, and small subunit (SSU) rDNA sequence data. Based on a combination of previous and present studies, detailed morphometric data and the improved diagnoses of both species are supplied here. In addition, molecular data of the two species are reported for the first time. Phylogenetic analyses based on SSU rDNA sequence data support the generic assignment of these two species. Conclusions Two insufficiently studied species, G. uninucleata and L. vorticella , are redefined using state-of-the-art methods. Previous reports on these two species are re-evaluated in the light of present findings.

A reassessment of excavated Tuber species from China based on morphology and ITS rDNA sequence data

Mycotaxon ◽  
2013 ◽  
Vol 124 (1) ◽  
pp. 155-163 ◽  
Author(s):  
Li Fan ◽  
Jin-Zhong Cao ◽  
Yu Li
Keyword(s):  
Sequence Data ◽  
Rdna Sequence ◽  
Its Rdna ◽  
Rdna Sequence Data

Two new Terfezia species from Southern Europe

Phytotaxa ◽  
2015 ◽  
Vol 230 (3) ◽  
pp. 239 ◽  
Author(s):  
Juan Julián Bordallo ◽  
Antonio Rodríguez ◽  
Vasileios Kounas ◽  
Francisco Camello ◽  
Mario Honrubia ◽  
...  
Keyword(s):  
New Species ◽  
Host Plants ◽  
Sequence Data ◽  
Rdna Sequence ◽  
Its Rdna ◽  
Similar Species ◽  
Southern Europe ◽  
Phylogenetic Data ◽  
Rdna Sequence Data ◽  
Two New Species

Two new species of Terfezia, Terfezia grisea and Terfezia cistophila, are documented from Spain and Greece, based on morphology and ITS-rDNA sequence data. Macro and micro descriptions with illustrations and ITS phylogenetic data for the two species are provided, which are discussed in relation to similar species in this genus and their host plants.

Tamhinispora saraburiensis sp. nov. (Tubeufiaceae, Dothideomycetes) on bamboo in Thailand based on morphology & phylogenetic analysis

Phytotaxa ◽  
2019 ◽  
Vol 402 (1) ◽  
pp. 1 ◽  
Author(s):  
SUJINDA SOMMAI ◽  
SALILAPORN NUANKAEW ◽  
PHONGSWAT KHAMSUNTORN ◽  
SATINEE SUETRONG ◽  
UMPAWA PINRUAN
Keyword(s):  
Phylogenetic Analysis ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Rdna Sequence ◽  
Lsu Rdna ◽  
The Family ◽  
Rdna Sequence Data

Tamhinispora saraburiensis sp. nov. was collected on decaying culms of bamboo in Saraburi Province, Thailand. Tamhinispora saraburiensis is characterized as dark brown to black, obpyriform, and dictyoseptate and has conidia with long appendages arising from the conidial tip. Phylogenetic analyses of the combined ITS and LSU rDNA sequence data demonstrated that this species formed distinctly from other species and that it belongs in the family Tubeufiaceae. Tamhinispora saraburiensis is therefore introduced here with a description, morphological illustrations and molecular data. A key to the species of Tamhinispora is provided.

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