cAMP Inhibits Inducible Nitric Oxide Synthase Expression and NF-κB-Binding Activity in Cultured Rat Hepatocytes

2001 ◽  
Vol 99 (2) ◽  
pp. 258-264 ◽  
Author(s):  
Brian G. Harbrecht ◽  
Bradley S. Taylor ◽  
Zhongfa Xu ◽  
Santhanam Ramalakshmi ◽  
Raymond W. Ganster ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Rat Hepatocytes ◽  
Binding Activity ◽  
Cultured Rat Hepatocytes ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals Natural antisense transcript stabilizes inducible nitric oxide synthase messenger RNA in rat hepatocytes

Hepatology ◽  
2007 ◽  
Vol 47 (2) ◽  
pp. 686-697 ◽  
Author(s):  
Kosuke Matsui ◽  
Mikio Nishizawa ◽  
Takashi Ozaki ◽  
Tominori Kimura ◽  
Iwao Hashimoto ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Messenger Rna ◽  
Antisense Transcript ◽  
Rat Hepatocytes ◽  
Natural Antisense Transcript ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Genipin Inhibits the Induction of Inducible Nitric Oxide Synthase Through the Inhibition of NF-κB Activation in Rat Hepatocytes

2017 ◽  
Vol 10 (4) ◽  
pp. 254-263 ◽  
Author(s):  
Richi Nakatake ◽  
Takumi Tsuda ◽  
Takashi Matsuura ◽  
Hirokazu Miki ◽  
Hidehiko Hishikawa ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Rat Hepatocytes ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Expression of inducible nitric oxide synthase in endotoxemic rat hepatocytes is dependent on the cellular glutathione status

Hepatology ◽  
1999 ◽  
Vol 29 (2) ◽  
pp. 421-426 ◽  
Author(s):  
Thera A. Vos ◽  
Harry van Goor ◽  
Leonore Tuyt ◽  
Alie de Jager-Krikken ◽  
Ron Leuvenink ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Rat Hepatocytes ◽  
Glutathione Status ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals Age alters cerebrovascular inflammation and effects of estrogen

2007 ◽  
Vol 292 (5) ◽  
pp. H2333-H2340 ◽  
Author(s):  
Lorraine Sunday ◽  
Christa Osuna ◽  
Diana N. Krause ◽  
Sue P. Duckles
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Dna Binding ◽  
Inducible Nitric Oxide Synthase ◽  
Binding Activity ◽  
Cerebral Vessels ◽  
Cerebral Blood Vessels ◽  
Dna Binding Activity ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

In young adult females, estrogen treatment suppresses the cerebrovascular inflammatory response; this is mediated in part via NF-κB, a key regulator of inflammatory genes. To examine whether age modifies effects of estrogen on vascular inflammation in the brain, female rats, 3 and 12 mo of age, were ovariectomized; half were treated with estrogen for 4 wk. Cerebral blood vessels were isolated from the animals at 4 and 13 mo of age. Inflammation was induced by LPS, either injected in vivo or incubated with isolated vessels ex vivo. Basal levels of cytoplasmic NF-κB were significantly higher in cerebral vessels of young rats, but the ratio of nuclear to cytoplasmic levels was greater in middle-aged animals. LPS exposure increased nuclear NF-κB DNA binding activity, protein levels of inducible nitric oxide synthase and cyclooxygenase-2, and production of nitric oxide and PGE2 in cerebral vessels. All effects of LPS were markedly greater in vessels from the older animals. Estrogen significantly inhibited the LPS-induced increase in NF-κB DNA binding activity in cerebral vessels from animals at both ages. In 4-mo-old rats, estrogen also significantly suppressed LPS induction of inducible nitric oxide synthase and cyclooxygenase-2 proteins, as well as production of nitric oxide and PGE2. In contrast, in 13-mo-old females, estrogen did not significantly affect these indexes of cerebrovascular inflammation. Thus the protective, anti-inflammatory effect of estrogen on cerebral blood vessels that is observed in young adults may be attenuated in aged animals, which exhibit a greater overall cerebrovascular response to inflammatory stimuli.

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