Interleukin-6 Production in Human Intestinal Epithelial Cells Increases in Association with the Heat Shock Response

1998 ◽  
Vol 77 (1) ◽  
pp. 40-44 ◽  
Author(s):  
Alexander A. Parikh ◽  
M.Ryan Moon ◽  
Christine D. Kane ◽  
Andrew L. Salzman ◽  
Josef E. Fischer ◽  
...  
Keyword(s):  
Heat Shock ◽  
Epithelial Cells ◽  
Heat Shock Response ◽  
Interleukin 6 ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells ◽  
Shock Response

scholarly journals Polyamines mediate glutamine-dependent induction of the intestinal epithelial heat shock response

2011 ◽  
Vol 301 (1) ◽  
pp. G181-G187 ◽  
Author(s):  
Yuji Iwashita ◽  
Toshio Sakiyama ◽  
Mark W. Musch ◽  
Mark J. Ropeleski ◽  
Hirohito Tsubouchi ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Epithelial Cells ◽  
Heat Shock Response ◽  
Intestinal Epithelial Cells ◽  
Critical Role ◽  
Intestinal Epithelial ◽  
Stress Induction ◽  
Polyamine Synthesis ◽  
Shock Response

Heat shock proteins (Hsps) are highly conserved proteins that play a role in cytoprotection and maintaining intestinal homeostasis. Glutamine is essential for the optimal induction of intestinal epithelial Hsp expression, but its mechanisms of action are incompletely understood. Glutamine is a substrate for polyamine synthesis and stimulates the activity of ornithine decarboxylase (ODC), a key enzyme for polyamine synthesis, in intestinal epithelial cells. Thus we investigated whether polyamines (putrescine, spermidine, or spermine) and their precursor ornithine mediate the induction of Hsp expression in IEC-18 rat intestinal epithelial cells. As previously observed, glutamine was required for heat stress induction of Hsp70 and Hsp25, although it had little effect under basal conditions. Under conditions of glutamine depletion, supplementation of ornithine or polyamines restored the heat-induced expression of Hsp70 and Hsp25. When ODC was inhibited by α-difluoromethylornithine (DFMO), an irreversible ODC inhibitor, the heat stress induction of Hsp70 and Hsp25 was decreased significantly, even in the presence of glutamine. Ornithine, polyamines, and DFMO did not modify the nuclear localization of heat shock transcription factor 1 (HSF-1). However, DFMO dramatically reduced glutamine-dependent HSF-1 binding to an oligonucleotide with heat shock elements (HSE), which was increased by glutamine. In addition, exogenous polyamines recovered the DNA-binding activity. These results indicate that polyamines play a critical role in the glutamine-dependent induction of the intestinal epithelial heat shock response through facilitation of HSF-1 binding to HSE.

INTERLEUKIN-6 SIGNAL TRANSDUCTION IN HUMAN INTESTINAL EPITHELIAL CELLS

Shock ◽  
2000 ◽  
Vol 13 (6) ◽  
pp. 435-440 ◽  
Author(s):  
Scott L. Pratt ◽  
Cora K. Ogle ◽  
Ju-Xian Mao ◽  
Wenzhu Zhao ◽  
Glenda Lovell ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Epithelial Cells ◽  
Interleukin 6 ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells

INTERLEUKIN-1β AND INTERFERON-γ REGULATE INTERLEUKIN-6 PRODUCTION IN CULTURED HUMAN INTESTINAL EPITHELIAL CELLS

Shock ◽  
1997 ◽  
Vol 8 (4) ◽  
pp. 249-255 ◽  
Author(s):  
Alexander A. Parikh ◽  
Andrew L. Salzman ◽  
Josef E. Fischer ◽  
Csaba Szabo ◽  
Per-Olof Hasselgren
Keyword(s):  
Epithelial Cells ◽  
Interleukin 6 ◽  
Intestinal Epithelial Cells ◽  
Interferon Γ ◽  
Interleukin 1Β ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells
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