Monitoring Biomolecular Interactions by Time-Lapse Atomic Force Microscopy

2000 ◽  
Vol 131 (3) ◽  
pp. 171-180 ◽  
Author(s):  
Martin Stolz ◽  
Daniel Stoffler ◽  
Ueli Aebi ◽  
Claire Goldsbury
Keyword(s):  
Atomic Force Microscopy ◽  
Time Lapse ◽  
Biomolecular Interactions ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Biomolecular Interactions Measured by Atomic Force Microscopy

2000 ◽  
Vol 79 (6) ◽  
pp. 3267-3281 ◽  
Author(s):  
Oscar H. Willemsen ◽  
Margot M.E. Snel ◽  
Alessandra Cambi ◽  
Jan Greve ◽  
Bart G. De Grooth ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Biomolecular Interactions ◽  
Force Microscopy ◽  
Atomic Force

Towards monitoring transport of single cargos across individual nuclear pore complexes by time-lapse atomic force microscopy

2010 ◽  
Vol 171 (2) ◽  
pp. 154-162 ◽  
Author(s):  
Ning-Ping Huang ◽  
Mike Stubenrauch ◽  
Joachim Köser ◽  
Nicole Taschner ◽  
Ueli Aebi ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Time Lapse ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Pore Complexes

scholarly journals Surface assembly and nanofabrication of 1,1,1-tris(mercaptomethyl)heptadecane on Au(111) studied with time-lapse atomic force microscopy

2014 ◽  
Vol 5 ◽  
pp. 26-35 ◽  
Author(s):  
Tian Tian ◽  
Burapol Singhana ◽  
Lauren E Englade-Franklin ◽  
Xianglin Zhai ◽  
T Randall Lee ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Self Assembly ◽  
Time Lapse ◽  
Scanning Probe ◽  
Self Assembled Monolayers ◽  
Force Microscopy ◽  
Atomic Force ◽  
Surface Assembly ◽  
Assembled Monolayers ◽  
Liquid Cell

The solution self-assembly of multidentate organothiols onto Au(111) was studied in situ using scanning probe nanolithography and time-lapse atomic force microscopy (AFM). Self-assembled monolayers (SAMs) prepared from dilute solutions of multidentate thiols were found to assemble slowly, requiring more than six hours to generate films. A clean gold substrate was first imaged in ethanolic media using liquid AFM. Next, a 0.01 mM solution of multidentate thiol was injected into the liquid cell. As time progressed, molecular-level details of the surface changes at different time intervals were captured by successive AFM images. Scanning probe based nanofabrication was accomplished using protocols of nanografting and nanoshaving with n-alkanethiols and a tridentate molecule, 1,1,1-tris(mercaptomethyl)heptadecane (TMMH). Nanografted patterns of TMMH could be inscribed within n-alkanethiol SAMs; however, the molecular packing of the nanopatterns was less homogeneous compared to nanopatterns produced with monothiolates. The multidentate molecules have a more complex assembly pathway than monothiol counterparts, mediated by sequential steps of forming S–Au bonds to the substrate.

Comparison study of live cells by atomic force microscopy, confocal microscopy, and scanning electrochemical microscopy

2007 ◽  
Vol 85 (3) ◽  
pp. 175-183 ◽  
Author(s):  
Xiaocui Zhao ◽  
Nils O Petersen ◽  
Zhifeng Ding
Keyword(s):  
Atomic Force Microscopy ◽  
Confocal Microscopy ◽  
Scanning Electrochemical Microscopy ◽  
Time Lapse ◽  
Live Cells ◽  
Tapping Mode ◽  
Force Microscopy ◽  
Atomic Force ◽  
Tapping Mode Afm ◽  
Electrochemical Microscopy

In this report, three kinds of scanning probe microscopy techniques, atomic force microscopy (AFM), confocal microscopy (CM), and scanning electrochemical microscopy (SECM), were used to study live cells in the physiological environment. Two model cell lines, CV-1 and COS-7, were studied. Time-lapse images were obtained with both contact and tapping mode AFM techniques. Cells were more easily scratched or moved by contact mode AFM than by tapping mode AFM. Detailed surface structures such as filamentous structures on the cell membrane can be obtained and easily discerned with tapping mode AFM. The toxicity of ferrocenemethanol (Fc) on live cells was studied by CM in reflection mode by recording the time-lapse images of controlled live cells and live cells with different Fc concentrations. No significant change in the morphology of cells was caused by Fc. Cells were imaged by SECM with Fc as the mediator at a biased potential of 0.35 V (vs. Ag/AgCl with a saturated KCl solution). Cells did not change visibly within 1 h, which indicated that SECM was a noninvasive technique and thus has a unique advantage for the study of soft cells, since the electrode scanned above the cells instead of in contact with them. Reactive oxygen species (ROS) generated by the cells were detected and images based on these chemical species were obtained. It is demonstrated that SECM can provide not only the topographical images but also the images related to the chemical or biochemical species released by the live cells.Key words: live cells, atomic force microscopy, confocal microscopy, scanning electrochemical microscopy.

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