Electron Microscopic Localization of LacZ Expression in the Proximal Convoluted Tubular Cells of the Kidney in Transgenic Mice Carrying Chimeric Erythropoietin/lacZ Gene Constructs

1997 ◽  
Vol 118 (3) ◽  
pp. 220-225 ◽  
Author(s):  
Mohammad A. Haidar ◽  
Francisco Loya ◽  
Yun Yang ◽  
Huai Lin ◽  
Armand Glassman ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Lacz Gene ◽  
Lacz Expression ◽  
Electron Microscopic ◽  
Tubular Cells ◽  
Microscopic Localization ◽  
Electron Microscopic Localization

scholarly journals Transgenic mice carrying the erythropoietin gene promoter linked to lacZ express the reporter in proximal convoluted tubule cells after hypoxia

Blood ◽  
1994 ◽  
Vol 84 (6) ◽  
pp. 1831-1836 ◽  
Author(s):  
F Loya ◽  
Y Yang ◽  
H Lin ◽  
E Goldwasser ◽  
M Albitar
Keyword(s):  
Transgenic Mice ◽  
Gene Promoter ◽  
Large Degree ◽  
Proximal Convoluted Tubule ◽  
Lacz Gene ◽  
Lacz Expression ◽  
Specific Expression ◽  
Tubular Cells ◽  
Dna Fragment ◽  
Significant Expression

Results on the localization of erythropoietin (Epo) synthesis in renal cells have been contradictory, implicating either interstitial or tubular cells. We fused the lacZ gene to a 7-kb DNA fragment of the mouse Epo gene encompassing a portion of the first intron, the first exon, and a 6-kb sequence of the 5′-flanking region. Transgenic mice carrying this construct show a low level of specific expression of the lacZ gene in proximal convoluted tubule (PCT) cells. Without hypoxia, no significant expression was detected in the liver. Hypoxia induced a large degree of lacZ expression, mainly in kidney PCT cells and to a lesser degree in liver. However, anemia induced lacZ expression in both kidney and liver. These findings indicate that, under these conditions, Epo is expressed in tubular cells, specifically PCT cells.

scholarly journals Transgenic mice carrying the erythropoietin gene promoter linked to lacZ express the reporter in proximal convoluted tubule cells after hypoxia

Blood ◽  
1994 ◽  
Vol 84 (6) ◽  
pp. 1831-1836 ◽  
Author(s):  
F Loya ◽  
Y Yang ◽  
H Lin ◽  
E Goldwasser ◽  
M Albitar
Keyword(s):  
Transgenic Mice ◽  
Gene Promoter ◽  
Large Degree ◽  
Proximal Convoluted Tubule ◽  
Lacz Gene ◽  
Lacz Expression ◽  
Specific Expression ◽  
Tubular Cells ◽  
Dna Fragment ◽  
Significant Expression

Abstract Results on the localization of erythropoietin (Epo) synthesis in renal cells have been contradictory, implicating either interstitial or tubular cells. We fused the lacZ gene to a 7-kb DNA fragment of the mouse Epo gene encompassing a portion of the first intron, the first exon, and a 6-kb sequence of the 5′-flanking region. Transgenic mice carrying this construct show a low level of specific expression of the lacZ gene in proximal convoluted tubule (PCT) cells. Without hypoxia, no significant expression was detected in the liver. Hypoxia induced a large degree of lacZ expression, mainly in kidney PCT cells and to a lesser degree in liver. However, anemia induced lacZ expression in both kidney and liver. These findings indicate that, under these conditions, Epo is expressed in tubular cells, specifically PCT cells.

The electron microscopic localization of nephrotoxic antibodies in isolated glomeruli

Pathology ◽  
1976 ◽  
Vol 8 (1) ◽  
pp. 73-80 ◽  
Author(s):  
I.P. McCausland ◽  
R.N. Seelye ◽  
J.B. Gavin ◽  
P.B. Herdson
Keyword(s):  
Electron Microscopic ◽  
Isolated Glomeruli ◽  
Microscopic Localization ◽  
Electron Microscopic Localization
Sign in / Sign up

Export Citation Format

Share Document