Angiotensin II Induces Nuclear Factor- κ B Activation in Cultured Neonatal Rat Cardiomyocytes Through Protein Kinase C Signaling Pathway

2000 ◽  
Vol 32 (10) ◽  
pp. 1767-1778 ◽  
Author(s):  
Patricia Rouet-Benzineb ◽  
Brigitte Gontero ◽  
Patrick Dreyfus ◽  
Chantal Lafuma
Keyword(s):  
Protein Kinase C ◽  
Angiotensin Ii ◽  
Protein Kinase ◽  
Signaling Pathway ◽  
Neonatal Rat ◽  
Nuclear Factor ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes ◽  
Kinase C ◽  
Nuclear Factor Κ B

Hypertrophic growth of cultured neonatal rat heart cells mediated by type 1 angiotensin II receptor

1994 ◽  
Vol 266 (6) ◽  
pp. H2443-H2451 ◽  
Author(s):  
S. Miyata ◽  
T. Haneda
Keyword(s):  
Protein Kinase C ◽  
Angiotensin Ii ◽  
Protein Kinase ◽  
Neonatal Rat ◽  
Heart Cells ◽  
Ang Ii ◽  
Angiotensin Ii Receptor ◽  
Hypertrophic Growth ◽  
Kinase C

Primary cultures of neonatal cardiac myocytes were used to determine the identity of second messengers that are involved in angiotensin II (ANG II) receptor-mediated effects on cardiac hypertrophy and the type of ANG II receptor that is involved in ANG II-induced cell growth. Treatment of myocytes with ANG II significantly increased the protein-to-DNA and the RNA-to-DNA ratios. ANG II accelerated rates of protein synthesis by 24.9%. Intracellular free calcium was transiently increased after ANG II exposure. The activity of protein kinase C in particulate fractions was transiently increased after exposure to ANG II but returned to control level. The activity of protein kinase C in the cytosol was significantly decreased at all times after exposure to ANG II. After ANG II treatment, the content of c-Fos mRNA was increased. The stimulatory effects of ANG II on these parameters were inhibited by the type 1 angiotensin II receptor (AT1) antagonist, losartan. These studies demonstrate that ANG II-induced hypertrophic growth is, at least in part, mediated through AT1 receptors.

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