Hypoxia Regulates Basal and Induced DNA Synthesis and Collagen Type I Production in Human Cardiac Fibroblasts: Effects of Transforming Growth Factor-β1, Thyroid Hormone, Angiotensin II and Basic Fibroblast Growth Factor

1997 ◽  
Vol 29 (8) ◽  
pp. 2233-2244 ◽  
Author(s):  
Augustine Agocha ◽  
Hyeon-Woo Lee ◽  
Mahboubeh Eghbali-Webb
Keyword(s):  
Growth Factor ◽  
Thyroid Hormone ◽  
Collagen Type ◽  
Transforming Growth Factor ◽  
Cardiac Fibroblasts ◽  
Collagen Type I ◽  
Transforming Growth Factor Β1 ◽  
Type I ◽  
Fibroblast Growth ◽  
Human Cardiac Fibroblasts

scholarly journals Collagen Type I Containing Hybrid Hydrogel Enhances Cardiomyocyte Maturation in a 3D Cardiac Model

Polymers ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 687 ◽  
Author(s):  
Sam G. Edalat ◽  
Yongjun Jang ◽  
Jongseong Kim ◽  
Yongdoo Park
Keyword(s):  
Growth Factor ◽  
Collagen Type ◽  
Transforming Growth Factor ◽  
Embryonic Stem ◽  
Collagen Type I ◽  
Transforming Growth Factor Β1 ◽  
Type I ◽  
Hybrid Hydrogel ◽  
Cardiac Model

In vitro maturation of cardiomyocytes in 3D is essential for the development of viable cardiac models for therapeutic and developmental studies. The method by which cardiomyocytes undergoes maturation has significant implications for understanding cardiomyocytes biology. The regulation of the extracellular matrix (ECM) by changing the composition and stiffness is quintessential for engineering a suitable environment for cardiomyocytes maturation. In this paper, we demonstrate that collagen type I, a component of the ECM, plays a crucial role in the maturation of cardiomyocytes. To this end, embryonic stem-cell derived cardiomyocytes were incorporated into Matrigel-based hydrogels with varying collagen type I concentrations of 0 mg, 3 mg, and 6 mg. Each hydrogel was analyzed by measuring the degree of stiffness, the expression levels of MLC2v, TBX18, and pre-miR-21, and the size of the hydrogels. It was shown that among the hydrogel variants, the Matrigel-based hydrogel with 3 mg of collagen type I facilitates cardiomyocyte maturation by increasing MLC2v expression. The treatment of transforming growth factor β1 (TGF-β1) or fibroblast growth factor 4 (FGF-4) on the hydrogels further enhanced the MLC2v expression and thereby cardiomyocyte maturation.

scholarly journals SPARC Regulates the Expression of Collagen Type I and Transforming Growth Factor-β1 in Mesangial Cells

1999 ◽  
Vol 274 (45) ◽  
pp. 32145-32152 ◽  
Author(s):  
Aleksandar Francki ◽  
Amy D. Bradshaw ◽  
James A. Bassuk ◽  
Chin C. Howe ◽  
William G. Couser ◽  
...  
Keyword(s):  
Growth Factor ◽  
Collagen Type ◽  
Transforming Growth Factor ◽  
Mesangial Cells ◽  
Collagen Type I ◽  
Transforming Growth Factor Β1 ◽  
Type I
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