Determination of Tartrazine and Ponceau-4R in Various Food Samples by Vierordt's Method and Ratio Spectra First-Order Derivative UV Spectrophotometry

2002 ◽  
Vol 15 (6) ◽  
pp. 667-683 ◽  
Author(s):  
Sacide Altιnöz ◽  
Suna Toptan
Keyword(s):  
Food Samples ◽  
Order Derivative ◽  
Uv Spectrophotometry ◽  
First Order ◽  
Ponceau 4R ◽  
Derivative Uv Spectrophotometry

APPLICATION OF UV-SPECTROPHOTOMETRY FOR DETERMINATION OF ZILEUTON IN BULK AND IN TABLETS

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (06) ◽  
pp. 40-44
Author(s):  
S. B. Ganorkar ◽  
◽  
D. M Dhumal ◽  
A. A Shirkhedkar
Keyword(s):  
Simple Zero ◽  
Zero Order ◽  
Order Derivative ◽  
Uv Spectrophotometry ◽  
Active Inhibitor ◽  
First Order ◽  
Ich Guidelines ◽  
Orally Active ◽  
Derivative Uv Spectrophotometry

Zileuton is an orally active inhibitor of 5-lipoxygenase and found to inhibit leukotrienes (LTB4 , LTC4, LTD4 , and LTE4) formation and well-known for the effective treatment of asthma. Rapid, economical and simple ‘Zero order UV-spectrophotometry’ and ‘First order derivative UV-spectrophotometry’ have been developed for estimation zileuton in bulk and in-house tablets. Zileuton showed maximum absorbance at 258.4 nm in 0.01M NaOH. For Zero order UV-spectrophotometric method (Method I), two wavelengths 243.4 nm and 271.0 nm were selected for the determination of area under curve (AUC). In First order derivative UV-spectrophotometry (Method II), two wavelengths 260.0 nm and 279.60 nm were selected for determination of AUC. For both these methods, zileuton followed linearity in the concentration range of 4 - 24 µg/ mL with correlation coefficient value of 0.999. Both methods were validated as per ICH guidelines.

scholarly journals Estimation of Tadalafil Using Derivative Spectrophotometry in Bulk Material and in Pharmaceutical Formulation

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Zamir G. Khan ◽  
Amod S. Patil ◽  
Atul A. Shirkhedkar
Keyword(s):  
Wavelength Range ◽  
Area Under Curve ◽  
Pharmaceutical Formulation ◽  
Second Order ◽  
Order Derivative ◽  
Uv Spectrophotometry ◽  
First Order ◽  
Beer's Law ◽  
Beer’S Law ◽  
Derivative Uv Spectrophotometry

Four simple, rapid, accurate, precise, reliable, and economical UV-spectrophotometric methods have been proposed for the determination of tadalafil in bulk and in pharmaceutical formulation. “Method A” is first order derivative UV spectrophotometry using amplitude, “method B” is first order derivative UV spectrophotometry using area under curve technique, “method C” is second order derivative UV spectrophotometry using amplitude, and “method D” is second order derivative UV spectrophotometry using area under curve technique. The developed methods have shown best results in terms of linearity, accuracy, precision, and LOD and LOQ for bulk drug and marketed formulation as well. In N,N-dimethylformamide, tadalafil showed maximum absorbance at 284 nm. For “method A” amplitude was recorded at 297 nm while for “method B” area under curve was integrated in the wavelength range of 290.60–304.40 nm. For “method C” amplitude was measured at 284 nm while for “method D” area under curve was selected in the wavelength range of 280.80–286.20 nm. For methods A and B, tadalafil obeyed Lambert-Beer’s law in the range of 05–50 μg/mL while for “methods C and D”, tadalafil obeyed Lambert-Beer’s law in the range of 20–70 μg/mL, and-for “methods A, B, C, and D” the correlation coefficients were found to be > than 0.999.

NOVEL ZERO ORDER UV-SPECTROPHOTOMETRIC AND FIRST ORDER DERIVATIVE UV-AUC METHODS FOR ESTIMATION OF ETOMIDATE IMPARTING HYDROTROPIC SOLUBLIZATION

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (04) ◽  
pp. 15-20
Author(s):  
Atul A. Shirkhedkar ◽  
◽  
Saurabh B. Ganorkar
Keyword(s):  
Water Solubility ◽  
Pharmaceutical Formulations ◽  
Zero Order ◽  
Order Derivative ◽  
Uv Spectrophotometry ◽  
First Order ◽  
Spectrophotometric Methods ◽  
Poor Water Solubility ◽  
Hydrotropic Agent

Simple, novel UV-spectrophotometric methods have been developed and validated for the estimation of etomidate imparting a hydrotropic solublization phenomenon. Etomidate is an imidazole derivative, approved as a short acting intravenous anesthetic having poor water solubility. As the UV-spectrophotometric estimation was not seen in any published literature, we have reported it here; zero order UV-spectrophotometry (Method I) and First Order Derivative UV-spectrophotometry (Method II) with the application of hydrotropy to counteract the poor water solubility of etomidate. The present research endeavour utilizes propylene glycol (PG) (2% v/v with distilled water) as the hydrotropic agent for the UV-spectrophotometric determination of etomidate. The drug was found to be stable in solution form with the aforementioned hydrotropic agent. Method I was carried out by measuring absorbance at λmax of 243.40 nm whilst for Method II, a uniform area between 245.40 - 268.40 nm was measured and plotted against concentration. The linearity range was obeyed to be in between 4 - 24 μg/mL with a correlation coefficient of 0.999 for both the developed methods. The non - interference of a hydrotropic agent ensures the specificity for proposed methods. The mean recovery of 99.60 (Method I) and 100.28 (Method II) reflects accuracy for developed methods. The method precision results were found to be within limit; expressed in % RSD; no more than 2.0%. The developed UV-spectrophotometric methods for the estimation of etomidate are simple, less time consuming and eco-friendly. The developed methods were validated according to ICH guidelines and proved statistically accurate, precise and sensitive, which can be extended to a routine determination of etomidate in bulk and pharmaceutical formulations.

QUANTITATIVE DETERMINATION OF RALOXIFENE HYDROCHLORIDE IN BULK AND TABLET FORMULATION BY UV-SPECTROPHOTOMETRY AND FIRST ORDER DERIVATIVE USING AREA UNDER CURVE METHOD

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (09) ◽  
pp. 40-43
Author(s):  
R. E Mutha ◽  
◽  
D. M Dhumal ◽  
P. H. Patil ◽  
A. A. Shirkhedkar ◽  
...  
Keyword(s):  
Spectrophotometric Method ◽  
Area Under Curve ◽  
Order Derivative ◽  
Uv Spectrophotometry ◽  
Uv Spectrum ◽  
First Order ◽  
Treatment And Prevention ◽  
Receptor Modulator ◽  
Raloxifene Hydrochloride

Raloxifene hydrochloride (RLF) is a non-steroidal, selective estrogen receptor modulator used in the treatment and prevention of osteoporosis in postmenopausal women. Two simple, rapid and economical 'zero order UV-spectrophotometric (method I)' and first order derivative spectrophotometric (method II) using area under curve (AUC) technique have been developed for estimation of RLF in bulk and tablets. In 0.1N NaOH, RLF showed maximum absorbance at 303.5 nm. In method I, AUC between two wavelengths 275.0 nm & 350.0 nm were selected. In method II, UV-spectrum of RLF was derivatized into first order and two wavelengths 303.0 nm and 385.0 nm were selected for determination of AUC. In both these methods, RLF obeyed linearity in the concentration range of 2-10 mcg/mL (r2>0.99). Proposed methods were applied for tablet formulations and amounts of RLF estimated by method I and method II were found to be 100.08±0.68 and 99.78±0.94, respectively. Both these methods were validated statistically and by recovery experiments.

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