A Novel y-type High Mr Glutenin Subunit (12·4t) Present in Triticum tauschii

2003 ◽  
Vol 37 (2) ◽  
pp. 253-256 ◽  
Author(s):  
M.C Gianibelli ◽  
R.G Solomon
Keyword(s):  
Glutenin Subunit ◽  
Triticum Tauschii

Molecular structure of a novel y-type HMW glutenin subunit gene present in Triticum tauschii

Euphytica ◽  
2005 ◽  
Vol 141 (1-2) ◽  
pp. 191-198 ◽  
Author(s):  
M. E. Hassani ◽  
M. C. Gianibelli ◽  
M. R. Shariflou ◽  
P. J. Sharp
Keyword(s):  
Molecular Structure ◽  
Glutenin Subunit ◽  
Subunit Gene ◽  
Triticum Tauschii ◽  
Hmw Glutenin Subunit ◽  
Hmw Glutenin

Genetic Diversity of High-Molecular-Weight Glutenin Subunit Composition in Chinese Wheat Landraces

2013 ◽  
Vol 38 (7) ◽  
pp. 1205-1211
Author(s):  
Xin XU ◽  
Xiao-Jun LI ◽  
Ling-Li ZHANG ◽  
Xiu-Quan LI ◽  
Xin-Ming YANG ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Weight ◽  
High Molecular Weight ◽  
Glutenin Subunit ◽  
Subunit Composition ◽  
Wheat Landraces ◽  
Chinese Wheat

scholarly journals Proteomic Determination of Low-Molecular-Weight Glutenin Subunit Composition in Aroona Near-Isogenic Lines and Standard Wheat Cultivars

2021 ◽  
Vol 22 (14) ◽  
pp. 7709
Author(s):  
Kyoungwon Cho ◽  
You-Ran Jang ◽  
Sun-Hyung Lim ◽  
Susan B. Altenbach ◽  
Yong Q. Gu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Allelic Variation ◽  
Reversed Phase ◽  
Glutenin Subunit ◽  
Low Molecular Weight ◽  
Near Isogenic Lines ◽  
Wheat Cultivars ◽  
Reliable Determination ◽  
Isogenic Lines

The low-molecular weight glutenin subunit (LMW-GS) composition of wheat (Triticum aestivum) flour has important effects on end-use quality. However, assessing the contributions of each LMW-GS to flour quality remains challenging because of the complex LMW-GS composition and allelic variation among wheat cultivars. Therefore, accurate and reliable determination of LMW-GS alleles in germplasm remains an important challenge for wheat breeding. In this study, we used an optimized reversed-phase HPLC method and proteomics approach comprising 2-D gels coupled with liquid chromatography–tandem mass spectrometry (MS/MS) to discriminate individual LMW-GSs corresponding to alleles encoded by the Glu-A3, Glu-B3, and Glu-D3 loci in the ‘Aroona’ cultivar and 12 ‘Aroona’ near-isogenic lines (ARILs), which contain unique LMW-GS alleles in the same genetic background. The LMW-GS separation patterns for ‘Aroona’ and ARILs on chromatograms and 2-D gels were consistent with those from a set of 10 standard wheat cultivars for Glu-3. Furthermore, 12 previously uncharacterized spots in ‘Aroona’ and ARILs were excised from 2-D gels, digested with chymotrypsin, and subjected to MS/MS. We identified their gene haplotypes and created a 2-D gel map of LMW-GS alleles in the germplasm for breeding and screening for desirable LMW-GS alleles for wheat quality improvement.

Composition of and variation in high- and low-molecular weight glutenin subunits, and omega gliadins in Ethiopian tetraploid wheat germplasm

2006 ◽  
Vol 4 (2) ◽  
pp. 134-143 ◽  
Author(s):  
Faris Hailu ◽  
Eva Johansson ◽  
Arnulf Merker ◽  
Getachew Belay ◽  
Harjit-Singh ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Tetraploid Wheat ◽  
Glutenin Subunit ◽  
Low Molecular Weight ◽  
Glutenin Subunits ◽  
Hmw Glutenin Subunits ◽  
Lmw Glutenin Subunits ◽  
Hmw Glutenin ◽  
Omega Gliadins ◽  
High Degree

A collection of 120 Ethiopian tetraploid wheat accessions was analysed for high-molecular weight (HMW) glutenin subunit, low-molecular weight (LMW) glutenin subunit and omega gliadin composition by SDS–PAGE. For the HMW glutenin subunits, a new allelic variant, 2****, was detected which has not been previously described at the Glu-A1 locus. A high proportion of Glu-A1x banding pattern was observed in durum wheat. For the Glu-B1 locus four different banding patterns were detected. Among those HMW glutenin subunits, 7+8 were the most common, while subunits 14+15 and 6+8 were found to be rare. A high degree of variation was evident for the LMW glutenin subunits and D-zone omega gliadins. The association of the composition of the gluten with quality has been discussed. This wide variation can be used in improving the quality of wheat and to widen its genetic base.

Real-time PCR-assisted selection of wheat plants transformed with HMW glutenin subunit genes

2005 ◽  
Vol 41 (1) ◽  
pp. 133-136 ◽  
Author(s):  
Valeria Terzi ◽  
Gabriela Pastori ◽  
Peter R. Shewry ◽  
Natale Di Fonzo ◽  
A. Michele Stanca ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Glutenin Subunit ◽  
Hmw Glutenin Subunit ◽  
Hmw Glutenin ◽  
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