Characterization of the Gene Encoding Mouse Retinoblastoma Binding Protein-7, a Component of Chromatin-Remodeling Complexes

Genomics ◽  
2002 ◽  
Vol 80 (4) ◽  
pp. 407-415 ◽  
Author(s):  
Jing Yang ◽  
Susan McLeskey Kiefer ◽  
Michael Rauchman
Keyword(s):  
Chromatin Remodeling ◽  
Binding Protein ◽  
Gene Encoding ◽  
Chromatin Remodeling Complexes

scholarly journals Identification and Functional Characterization of Protest SWI/SNF and ISWI Complexes

2021 ◽  
Author(s):  
Alejandro Saettone Chipana
Keyword(s):  
Chromatin Remodeling ◽  
Tetrahymena Thermophila ◽  
Affinity Purification ◽  
Functional Characterization ◽  
Peptide Array ◽  
Interacting Proteins ◽  
Silent Chromatin ◽  
Lysine Residues ◽  
Chromatin Remodeling Complexes

The thesis aims to identify and initiate functional characterization of the SWI/SNF and ISWI complexes in Tetrahymena thermophila. Through affinity purification of the conserved subunit Snf5 followed by mass spectrometry (AP-MS), I identified the first SWI/SNF complex in protists. One of the subunits I found is a small bromodomain containing protein named Ibd1. Through AP-MS of Ibd1 I found Ibd1 is versatile and interacts with several additional chromatin remodeling complexes. Bromodomains are known to have affinity for acetylated lysine residues within proteins such as histones. A peptide array experiment suggests that Ibd1 also has affinity for acetylated chromatin. Indirect immunofluorescence (IF) of Ibd1 hints at a role in transcription. My analysis of Tetrahymena Iswi1 shows expression during meiosis, vegetative growth and starvation. IF data shows its localization is consistent with Iswi1 function in mitosis/meiosis or maintenance of silent chromatin. AP-MS of ISW1 discovered several interacting proteins of unknown function.

Human CCAAT/Enhancer-Binding Protein β Interacts with Chromatin Remodeling Complexes of the Imitation Switch Subfamily

Biochemistry ◽  
2012 ◽  
Vol 51 (5) ◽  
pp. 952-962 ◽  
Author(s):  
Ximena P. Steinberg ◽  
Matias I. Hepp ◽  
Yaiza Fernández García ◽  
Tamaki Suganuma ◽  
Selene K. Swanson ◽  
...  
Keyword(s):  
Chromatin Remodeling ◽  
Binding Protein ◽  
Enhancer Binding Protein ◽  
Ccaat Enhancer Binding Protein ◽  
Chromatin Remodeling Complexes

scholarly journals Characterization of a Sinorhizobium melilotiATP-Binding Cassette Histidine Transporter Also Involved in Betaine and Proline Uptake

2000 ◽  
Vol 182 (13) ◽  
pp. 3717-3725 ◽  
Author(s):  
Eric Boncompagni ◽  
Laurence Dupont ◽  
Tam Mignot ◽  
Magne Østeräs ◽  
Annie Lambert ◽  
...  
Keyword(s):  
Glycine Betaine ◽  
Sinorhizobium Meliloti ◽  
Binding Protein ◽  
Compatible Solutes ◽  
Site Directed Mutagenesis ◽  
Uptake System ◽  
Periplasmic Binding Protein ◽  
Gene Encoding ◽  
Inhibition Of Growth

ABSTRACT The symbiotic soil bacterium Sinorhizobium melilotiuses the compatible solutes glycine betaine and proline betaine for both protection against osmotic stress and, at low osmolarities, as an energy source. A PCR strategy based on conserved domains in components of the glycine betaine uptake systems from Escherichia coli(ProU) and Bacillus subtilis (OpuA and OpuC) allowed us to identify a highly homologous ATP-binding cassette (ABC) binding protein-dependent transporter in S. meliloti. This system was encoded by three genes (hutXWV) of an operon which also contained a fourth gene (hutH2) encoding a putative histidase, which is an enzyme involved in the first step of histidine catabolism. Site-directed mutagenesis of the gene encoding the periplasmic binding protein (hutX) and of the gene encoding the cytoplasmic ATPase (hutV) was done to study the substrate specificity of this transporter and its contribution in betaine uptake. These mutants showed a 50% reduction in high-affinity uptake of histidine, proline, and proline betaine and about a 30% reduction in low-affinity glycine betaine transport. When histidine was used as a nitrogen source, a 30% inhibition of growth was observed inhut mutants (hutX and hutH2). Expression analysis of the hut operon determined using ahutX-lacZ fusion revealed induction by histidine, but not by salt stress, suggesting this uptake system has a catabolic role rather than being involved in osmoprotection. To our knowledge, Hut is the first characterized histidine ABC transporter also involved in proline and betaine uptake.

Cloning and characterization of an immunoreactive gene encoding a calcium-binding protein from Naegleria fowleri

2004 ◽  
Vol 137 (1) ◽  
pp. 169-173 ◽  
Author(s):  
Seok-Ryoul Jeong ◽  
Myung-Soo Cho ◽  
Sun Park ◽  
Kyongmin Hwang Kim ◽  
Kyoung-Ju Song ◽  
...  
Keyword(s):  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Naegleria Fowleri ◽  
Gene Encoding ◽  
Nægleria Fowleri

Cloning and characterization of a gene encoding ABP57, a soluble auxin-binding protein

2009 ◽  
Vol 3 (4) ◽  
pp. 293-299 ◽  
Author(s):  
Keunpyo Lee ◽  
Myung-Il Kim ◽  
Yu-Jihn Kwon ◽  
Minkyun Kim ◽  
Yong-Sam Kim ◽  
...  
Keyword(s):  
Binding Protein ◽  
Gene Encoding ◽  
Auxin Binding ◽  
Auxin Binding Protein

Structure and characterization of the gene encoding the ferredoxin-NADP reductase-binding protein from Zea mays L.

Gene ◽  
1994 ◽  
Vol 147 (2) ◽  
pp. 205-208 ◽  
Author(s):  
Silvina Pessino ◽  
Carme Caelles ◽  
Pere Puigdomènech ◽  
Rubén H. Vallejos
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Binding Protein ◽  
Gene Encoding
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