The Exon/Intron Organization and Chromosomal Mapping of the Mouse ADAMTS-1 Gene Encoding an ADAM Family Protein with TSP Motifs

Genomics ◽  
1997 ◽  
Vol 46 (3) ◽  
pp. 466-471 ◽  
Author(s):  
Kouji Kuno ◽  
Hisashi Iizasa ◽  
Shinsuke Ohno ◽  
Kouji Matsushima
Keyword(s):  
Chromosomal Mapping ◽  
Gene Encoding ◽  
Family Protein ◽  
Adam Family

cDNA cloning and chromosomal mapping of the gene encoding adenylate kinase 2 from Drosophila melanogaster

2000 ◽  
Vol 1490 (1-2) ◽  
pp. 109-114 ◽  
Author(s):  
Takafumi Noma ◽  
Ryutaro Murakami ◽  
Yasuhiro Yamashiro ◽  
Koichi Fujisawa ◽  
Sachie Inouye ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Cdna Cloning ◽  
Adenylate Kinase ◽  
Chromosomal Mapping ◽  
Gene Encoding

scholarly journals Expression of a Gene Encoding Trypanosoma congolense Putative Abc1 Family Protein is Developmentally Regulated

2005 ◽  
Vol 67 (2) ◽  
pp. 157-164 ◽  
Author(s):  
Waren N. BATICADOS ◽  
William H. WITOLA ◽  
Noboru INOUE ◽  
Jung-Yeon, KIM ◽  
Noritaka KUBOKI ◽  
...  
Keyword(s):  
Trypanosoma Congolense ◽  
Developmentally Regulated ◽  
Gene Encoding ◽  
Family Protein

scholarly journals BrucellaPeriplasmic Protein EipB Is a Molecular Determinant of Cell Envelope Integrity and Virulence

2019 ◽  
Vol 201 (12) ◽  
Author(s):  
Julien Herrou ◽  
Jonathan W. Willett ◽  
Aretha Fiebig ◽  
Daniel M. Czyż ◽  
Jason X. Cheng ◽  
...  
Keyword(s):  
Cell Envelope ◽  
Mammalian Host ◽  
Periplasmic Protein ◽  
Periplasmic Space ◽  
Gene Encoding ◽  
Tetratricopeptide Repeats ◽  
Content Type ◽  
Family Protein ◽  
Synthetically Lethal

ABSTRACTThe Gram-negative cell envelope is a remarkable structure with core components that include an inner membrane, an outer membrane, and a peptidoglycan layer in the periplasmic space between. Multiple molecular systems function to maintain integrity of this essential barrier between the interior of the cell and its surrounding environment. We show that a conserved DUF1849 family protein, EipB, is secreted to the periplasmic space ofBrucellaspecies, a monophyletic group of intracellular pathogens. In the periplasm, EipB folds into an unusual 14-stranded β-spiral structure that resembles the LolA and LolB lipoprotein delivery system, though the overall fold of EipB is distinct from LolA/LolB. Deletion ofeipBresults in defects inBrucellacell envelope integrityin vitroand in maintenance of spleen colonization in a mouse model ofBrucella abortusinfection. Transposon disruption ofttpA, which encodes a periplasmic protein containing tetratricopeptide repeats, is synthetically lethal witheipBdeletion.ttpAis a reported virulence determinant inBrucella, and our studies ofttpAdeletion and overexpression strains provide evidence that this gene also contributes to cell envelope function. We conclude thateipBandttpAfunction in theBrucellaperiplasmic space to maintain cell envelope integrity, which facilitates survival in a mammalian host.IMPORTANCEBrucellaspecies cause brucellosis, a global zoonosis. A gene encoding a conserved DUF1849-family protein, which we have named EipB, is present in all sequencedBrucellaand several other genera in the classAlphaproteobacteria. The manuscript provides the first functional and structural characterization of a DUF1849 protein. We show that EipB is secreted to the periplasm where it forms a spiral-shaped antiparallel β protein that is a determinant of cell envelope integrityin vitroand virulence in an animal model of disease.eipBgenetically interacts withttpA, which also encodes a periplasmic protein. We propose that EipB and TtpA function as part of a system required for cell envelope homeostasis in selectAlphaproteobacteria.

Genomic organization, promoter analysis, and chromosomal mapping of the mouse gene encoding Cdk9

2000 ◽  
Vol 78 (1) ◽  
pp. 170-178 ◽  
Author(s):  
Luigi Bagella ◽  
Peter Stiegler ◽  
Antonio De Luca ◽  
Linda D. Siracusa ◽  
Antonio Giordano
Keyword(s):  
Promoter Analysis ◽  
Genomic Organization ◽  
Mouse Gene ◽  
Chromosomal Mapping ◽  
Gene Encoding

Molecular Cloning and Chromosomal Mapping of the Mouse Gene Encoding Cyclin-Dependent Kinase 5 Regulatory Subunit p35

Genomics ◽  
1996 ◽  
Vol 35 (2) ◽  
pp. 372-375 ◽  
Author(s):  
Toshio Ohshima ◽  
Christine A. Kozak ◽  
James W. Nagle ◽  
Harish C. Pant ◽  
Roscoe O. Brady ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Mouse Gene ◽  
Chromosomal Mapping ◽  
Regulatory Subunit ◽  
Cyclin Dependent Kinase ◽  
Gene Encoding ◽  
Cyclin Dependent Kinase 5
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