Cloning and Characterization of an Arginine Vasotocin Receptor from the Euryhaline Flounder Platichthys flesus

2001 ◽  
Vol 122 (3) ◽  
pp. 312-319 ◽  
Author(s):  
J.M. Warne
Keyword(s):  
Arginine Vasotocin ◽  
Platichthys Flesus ◽  
Arginine Vasotocin Receptor

scholarly journals A monoclonal antibody to vasopressin: preparation, characterization, and application in immunocytochemistry.

1982 ◽  
Vol 30 (12) ◽  
pp. 1249-1260 ◽  
Author(s):  
A Hou-Yu ◽  
P H Ehrlich ◽  
G Valiquette ◽  
D L Engelhardt ◽  
W H Sawyer ◽  
...  
Keyword(s):  
Amino Acids ◽  
Monoclonal Antibody ◽  
Aromatic Amino Acids ◽  
Arginine Vasotocin ◽  
Antigenic Determinants ◽  
Tissue Fixation ◽  
Extensive Investigation ◽  
Carrier Proteins ◽  
The Subject

The hypothalamo-neurohypophysial system, containing the hormones oxytocin (OT) and vasopressin (VP) and their associated carrier proteins, the neurophysins (NPS), has been the subject of extensive investigation for more than 40 years. This system has been reinvestigated during the last decade by application of immunocytochemical methods employing the rabbit antisera to the hormones and NPS. In this study we describe the preparation and characterization of a monoclonal antibody to VP and its application in immunohistochemistry. The antibody did not cross-react with OT or arginine vasotocin (AVT). Its antigenic determinants as characterized by absorption with various VP analogs included two aromatic amino acids: Phe in position 3, and to a lesser extent Tyr in 2. Tissue fixation with formaldehyde resulted in inadequate immunostaining as compared to glutaraldehyde, most likely due to interference with the aromatic amino acid determinants by the former fixative.

scholarly journals Low Inducibility of CYP1A Activity by Polychlorinated Biphenyls (PCBs) in Flounder (Platichthys flesus): Characterization of the Ah Receptor and the Role of CYP1A Inhibition

1998 ◽  
Vol 43 (2) ◽  
pp. 161-171 ◽  
Author(s):  
H. T. Besselink ◽  
M. S. Denison ◽  
M. E. Hahn ◽  
S. I. Karchner ◽  
A. D. Vethaak ◽  
...  
Keyword(s):  
Polychlorinated Biphenyls ◽  
Ah Receptor ◽  
Platichthys Flesus ◽  
Cyp1a Activity
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