Dietary vitamin E and rainbow trout (Oncorhynchus mykiss) phagocyte functions: effect on gut and on head kidney leucocytes

2001 ◽  
Vol 11 (1) ◽  
pp. 1-13 ◽  
Author(s):  
P. Clerton ◽  
D. Troutaud ◽  
V. Verlhac ◽  
J. Gabaudan ◽  
P. Deschaux
Keyword(s):  
Rainbow Trout ◽  
Vitamin E ◽  
Oncorhynchus Mykiss ◽  
Head Kidney ◽  
Dietary Vitamin ◽  
Rainbow Trout Oncorhynchus Mykiss

Dietary vitamin E and the response of rainbow trout, Oncorhynchus mykiss (Walbaum), to infection with Yersinia ruckeri

1992 ◽  
Vol 41 (6) ◽  
pp. 1037-1041 ◽  
Author(s):  
M. D. Furones ◽  
D. J. Alderman ◽  
D. Bucke ◽  
T. C. Fletcher ◽  
D. Knox ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Vitamin E ◽  
Oncorhynchus Mykiss ◽  
Dietary Vitamin ◽  
Yersinia Ruckeri ◽  
Rainbow Trout Oncorhynchus Mykiss

scholarly journals Optimisation of the lymphocyte proliferation assay in rainbow trout (Oncorhynchus mykiss)

2019 ◽  
Vol 64 (No. 12) ◽  
pp. 547-557
Author(s):  
H Minarova ◽  
M Palikova ◽  
J Mares ◽  
E Syrova ◽  
J Blahova ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Bovine Serum ◽  
Lymphocyte Proliferation ◽  
Head Kidney ◽  
Pokeweed Mitogen ◽  
Lymphocyte Proliferation Assay ◽  
Proliferation Assay ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Foetal Bovine Serum

The lymphocyte proliferation assay is a valuable method used for the evaluation of the fish immune system. However, there are many variations and optimal results are not always obtained. Unification is necessary to ensure the comparability between different studies. The aim of this study was to optimise the lymphocyte proliferation assay in rainbow trout (Oncorhynchus mykiss). This goal included the determination of the optimal incubation length, serum type, incubation temperature, type of mitogen and its concentration, and anticoagulant. The peripheral blood and head kidney lymphocytes were isolated by density gradient centrifugation. Subsequently, the cells were incubated for 3–8 days with different mitogens (pokeweed mitogen 5, 10 and 50 µg/ml, concanavalin A 1, 10 and 20 µg/ml, phytohaemagglutinin 25, 50 and 100 µg/ml, lipopolysaccharide 1, 50 and 100 µg/ml). The use of the different serum types (foetal bovine serum, trout serum), incubation temperatures (10–20 °C) and anticoagulants (heparin, EDTA) was compared. Labelled thymidine was used to evaluate the assay. The best results were obtained after seven days of incubation at 15 °C with foetal bovine serum (FBS). The head kidney lymphocytes showed the highest proliferative response with 50 µg/ml phytohaemagglutinin. With the peripheral blood lymphocytes (heparin and EDTA), the best results were obtained with 50 µg/ml pokeweed mitogen. The highest proliferation levels were detected with heparinised blood. In conclusion, optimisation of this assay contributes to the improved assessment of the rainbow trout immune function.

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