Isolation and characterization of shiga toxin-producing Escherichia coli from meat and dairy products

2002 ◽  
Vol 19 (4) ◽  
pp. 389-398 ◽  
Author(s):  
N Al-Gallas
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Dairy Products ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Sorbitol-Fermenting Shiga Toxin (Verocytotoxin)-Producing Escherichia coli O157:Hßtrains in the Czech Republic

1998 ◽  
Vol 36 (7) ◽  
pp. 2135-2137 ◽  
Author(s):  
Martina Bielaszewska ◽  
Herbert Schmidt ◽  
Mohamed A. Karmali ◽  
Rasik Khakhria ◽  
Jan Janda ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Czech Republic ◽  
Shiga Toxin ◽  
Escherichia Coli O157 ◽  
The Czech Republic ◽  
Isolation And Characterization ◽  
First Finding ◽  
Uremic Syndrome ◽  
Genotypic Characteristics

Two sorbitol-fermenting (SF) Shiga toxin-producingEscherichia coli (STEC) O157:H− strains were isolated from patients with hemolytic-uremic syndrome in the Czech Republic in 1995. Their phenotypic and genotypic characteristics and genomic DNA fingerprints were identical or closely related to those of SF STEC O157:H− strains isolated in Germany in 1988 to 1997. This indicates that the Czech isolates belong to the SF STEC O157 clone which is widespread in Germany. It is the first finding of the clone outside Germany.

Isolation and characterization of Shiga toxin-producing Escherichia coli O157:H7 and non-O157 from beef carcasses at a slaughter plant in Mexico

2007 ◽  
Vol 113 (2) ◽  
pp. 237-241 ◽  
Author(s):  
J.J. Varela-Hernández ◽  
E. Cabrera-Diaz ◽  
M.A. Cardona-López ◽  
L.M. Ibarra-Velázquez ◽  
H. Rangel-Villalobos ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Escherichia Coli O157 ◽  
Isolation And Characterization ◽  
Beef Carcasses

Isolation and Characterization of Shiga Toxin–Producing Escherichia coli (STEC) in Retail Edible Beef By-products

2012 ◽  
Vol 9 (2) ◽  
pp. 145-149 ◽  
Author(s):  
Jae-Hoon Lee ◽  
Ji-Yeon Hyeon ◽  
Yun-Gyeong Kim ◽  
Jung-Whan Chon ◽  
Jun-Ho Park ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Isolation And Characterization ◽  
By Products

Isolation and characterization of Shiga toxin-producing Escherichia coli from precooked sausages (morcillas)

2006 ◽  
Vol 23 (3) ◽  
pp. 283-288 ◽  
Author(s):  
Juan M. Oteiza ◽  
Isabel Chinen ◽  
Elizabeth Miliwebsky ◽  
Marta Rivas
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of enteropathogenic and enterotoxinogenic Escherichia coli from dairy products consumed in Burkina Faso

2017 ◽  
Vol 11 (13) ◽  
pp. 537-545 ◽  
Author(s):  
Serge Bagre Touwendsida ◽  
Sambe-Ba Bissoume ◽  
Bawa Ibrahim Hadiza ◽  
Bsadjo Tchamba Gertrude ◽  
Dembele Rene ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Burkina Faso ◽  
Dairy Products ◽  
Isolation And Characterization

Detection, Isolation, and Characterization of Shiga Toxin–Producing Escherichia coli in Flour

2019 ◽  
Vol 82 (1) ◽  
pp. 164-167 ◽  
Author(s):  
PATRICK KINDLE ◽  
MAGDALENA NÜESCH-INDERBINEN ◽  
NICOLE CERNELA ◽  
ROGER STEPHAN
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Pcr Amplification ◽  
The United States ◽  
The European Union ◽  
Conventional Pcr ◽  
E Coli ◽  
Isolation And Characterization

ABSTRACT Wheat flour has recently been described as a novel vehicle for transmission of Shiga toxin–producing Escherichia coli (STEC). Very recently, an outbreak of STEC O121 and STEC O26 infections was linked to flour in the United States. The aim of the present study was to generate baseline data for the occurrence of STEC in flour samples from different retailers in Switzerland. In total, 70 flour samples were analyzed. After enrichment, the samples were screened for stx1 and stx2 by the Assurance GDS MPX ID assay. STEC strains were isolated and serotyped by the E. coli SeroGenoTyping AS-1 kit. The determination of stx subtypes was performed with conventional PCR amplification. Screening for eae, aggR, elt, and estIa/Ib was performed by real-time PCR. Nine (12.9%) of the flour samples tested positive for stx by PCR. STEC was recovered from eight (88.9%) of the positive samples. Two isolates were STEC O11:H48 harboring stx1c/stx1d, two were O146:H28 containing stx2b, one was O103:H2 containing stx1a and eae, and three were O nontypeable: Ont:H12 (stx2a), Ont:H14 (stx2a/stx2g), and Ont:H31 (stx1c/stx1d). STEC O103 belongs to the “top five” serogroups of human pathogenic STEC in the European Union, and STEC O146 is frequently isolated from diseased humans in Switzerland. Our results show that flour may be contaminated with a variety of STEC serogroups. Consumption of raw or undercooked flour may constitute a risk for STEC infection.

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