Isolation and Characterization of the Mating-Type Idiomorphs from the Wheat Septoria Leaf Blotch Fungus Mycosphaerella graminicola

2002 ◽  
Vol 35 (3) ◽  
pp. 277-286 ◽  
Author(s):  
Cees Waalwijk ◽  
Odette Mendes ◽  
Els C.P. Verstappen ◽  
Maarten A. de Waard ◽  
Gert H.J. Kema
Keyword(s):  
Mating Type ◽  
Mycosphaerella Graminicola ◽  
Septoria Leaf Blotch ◽  
Leaf Blotch ◽  
Isolation And Characterization

Selection and characterization of Argentine isolates of Trichoderma harzianum for effective biocontrol of Septoria leaf blotch of wheat

2016 ◽  
Vol 32 (3) ◽  
Author(s):  
Marina C. Stocco ◽  
Cecilia I. Mónaco ◽  
Cecilia Abramoff ◽  
Gladys Lampugnani ◽  
Graciela Salerno ◽  
...  
Keyword(s):  
Trichoderma Harzianum ◽  
Septoria Leaf Blotch ◽  
Leaf Blotch

Characterization of mat A-2, mat A-3 and ΔmatA Mating-Type Mutants of Neurospora crassa

Genetics ◽  
1998 ◽  
Vol 148 (3) ◽  
pp. 1069-1079 ◽  
Author(s):  
Adlane V-B Ferreira ◽  
Zhiqiang An ◽  
Robert L Metzenberg ◽  
N Louise Glass
Keyword(s):  
Neurospora Crassa ◽  
Mating Type ◽  
Sexual Development ◽  
Vegetative Growth ◽  
Double Mutant ◽  
Sexual Cycle ◽  
Wild Type ◽  
Type Locus ◽  
Isolation And Characterization

AbstractThe mating-type locus of Neurospora crassa regulates mating identity and entry into the sexual cycle. The mat A idiomorph encodes three genes, mat A-1, mat A-2, and mat A-3. Mutations in mat A-1 result in strains that have lost mating identity and vegetative incompatibility with mat a strains. A strain containing mutations in both mat A-2 and mat A-3 is able to mate, but forms few ascospores. In this study, we describe the isolation and characterization of a mutant deleted for mat (ΔmatA), as well as mutants in either mat A-2 or mat A-3. The ΔmatA strain is morphologically wild type during vegetative growth, but it is sterile and heterokaryon compatible with both mat A and mat a strains. The mat A-2 and mat A-3 mutants are also normal during vegetative growth, mate as a mat A strain, and produce abundant biparental asci in crosses with mat a, and are thus indistinguishable from a wild-type mat A strain. These data and the fact that the mat A-2 mat A-3 double mutant makes few asci with ascospores indicate that MAT A-2 and MAT A-3 are redundant and may function in the same pathway. Analysis of the expression of two genes (sdv-1 and sdv-4) in the various mat mutants suggests that the mat A polypeptides function in concert to regulate the expression of some sexual development genes.

Isolation and characterization of the mating type locus of Mycosphaerella fijiensis, the causal agent of black leaf streak disease of banana

2007 ◽  
Vol 8 (1) ◽  
pp. 111-120 ◽  
Author(s):  
LAURA CONDE-FERRÁEZ ◽  
CEES WAALWIJK ◽  
BLONDY B. CANTO-CANCHÉ ◽  
GERT H. J. KEMA ◽  
PEDRO W. CROUS ◽  
...  
Keyword(s):  
Mating Type ◽  
Causal Agent ◽  
Mycosphaerella Fijiensis ◽  
Type Locus ◽  
Mating Type Locus ◽  
Isolation And Characterization ◽  
Black Leaf Streak ◽  
Black Leaf Streak Disease

scholarly journals Characterization of Zymoseptoria tritici populations in Belarus by morphologic and cultural features

2021 ◽  
Vol 104 (4) ◽  
pp. 223-225
Author(s):  
N. A. Krupenko* ◽  
I. N. Odintsova
Keyword(s):  
Phenotypic Diversity ◽  
Southern Population ◽  
Zymoseptoria Tritici ◽  
Northern Population ◽  
Septoria Leaf Blotch ◽  
Leaf Blotch ◽  
The North ◽  
Shannon’S Index ◽  
Cultural Features

Septoria leaf blotch caused by Zymoseptoria tritici is one of the most harmful diseases in Belarus. Isolates of the pathogen were obtained from northern, central and southern populations: in 2018–2019, whichvaried significantly among in the structure of colonies. The rate of fungal isolates forming yeast-like colonies decreased from 55.0 % in the North of Belarus to 6.7 % in the South, whereas incidence of filamentous isolates increased from 31.3 % to 80.0 %, respectively. In the northern population, phenotypic diversity was high, while in the southern population it was the lowest (Shannon’s index was 1.53 and 1.14, respectively).

scholarly journals ISOLATION AND CHARACTERIZATION OF PERITHECIAL DEVELOPMENT MUTANTS IN NEUROSPORA

Genetics ◽  
1978 ◽  
Vol 88 (1) ◽  
pp. 27-47
Author(s):  
Thomas E Johnson
Keyword(s):  
Growth Rate ◽  
Mating Type ◽  
Temperature Sensitive ◽  
Uv Mutagenesis ◽  
Lower Growth ◽  
Type Locus ◽  
Isolation And Characterization ◽  
Lower Growth Rate ◽  
Complementation Groups

ABSTRACT The isolation and characterization of mutants that block perithecial development in Neurospora crassa are described. Several classes of mutants have been isolated after UV mutagenesis, and those that block perithecial development when used as the female (protoperithecial) component of a cross have been further characterized. These mutants fall into 29 complementation groups. Twelve of the 33 mutants block development at the protoperithecial stage; no other clustering of block points is observed. Many of the mutants show an altered vegetative growth rate as well; in several mutants this lower growth rate cosegregates with the female sterile phenotype. Only one mutant also blocks development of the perithecium when used as the conidial parent. None of the mutants are temperature sensitive; two can be suppressed by growth on a complete crossing medium. There is no indication that the mutants are at or in the mating-type locus, nor are any of the mutants mating-type specific. Genetic mosaics have been formed using mixtures of mutant and marked wild-type nuclei; no mutants are cell autonomous by this criterion. The significance of these results in terms of "developmental" mutants isolated in other organisms and in relation to models of eukaryotic development is discussed.

scholarly journals Mating-type control in Saccharomyces cerevisiae: isolation and characterization of mutants defective in repression by a1-alpha 2.

1989 ◽  
Vol 9 (10) ◽  
pp. 4523-4530 ◽  
Author(s):  
S Harashima ◽  
A M Miller ◽  
K Tanaka ◽  
K Kusumoto ◽  
K Tanaka ◽  
...  
Keyword(s):  
Mating Type ◽  
Translation Initiation ◽  
Genetic Background ◽  
Diploid Cell ◽  
Complementation Group ◽  
Negative Regulator ◽  
The Third ◽  
Isolation And Characterization ◽  
Translation Initiation Codon

The alpha 2 protein, the product of the MAT alpha 2 cistron, represses various genes specific to the a mating type (alpha 2 repression), and when combined with the MATa1 gene product, it represses MAT alpha 1 and various haploid-specific genes (a1-alpha 2 repression). One target of a1-alpha 2 repression is RME1, which is a negative regulator of a/alpha-specific genes. We have isolated 13 recessive mutants whose a1-alpha 2 repression is defective but which retain alpha 2 repression in a genetic background of ho MATa HML alpha HMRa sir3 or ho MAT alpha HMRa HMRa sir3. These mutations can be divided into three different classes. One class contains a missense mutation, designated hml alpha 2-102, in the alpha 2 cistron of HML, and another class contains two mat alpha 2-202, in the MAT alpha locus. These three mutants each have an amino acid substitution of tyrosine or acid substitution of tyrosine or phenylalanine for cysteine at the 33rd codon from the translation initiation codon in the alpha 2 cistron of HML alpha or MAT alpha. The remaining 10 mutants make up the third class and form a single complementation group, having mutations designated aar1 (a1-alpha 2 repression), at a gene other than MAT, HML, HMR, RME1, or the four SIR genes. Although a diploid cell homozygous for the aarl and sir3 mutations and for the MATa, HML alpha, and HMRa alleles showed alpha mating type, it could sporulate and gave rise to asci containing four alpha mating-type spores. These facts indicate that the domain for alpha2 repression is separable from that for a1-alpha2 protein interaction or complex formation in the alpha2 protein and that an additional regulation gene, AAR1, is associated with the a1-alpha2 repression of the alpha1 cistron and haploid-specific genes.

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