Characterization of the ABC Transporter Genes MgAtr1 and MgAtr2 from the Wheat Pathogen Mycosphaerella graminicola

2000 ◽  
Vol 30 (2) ◽  
pp. 115-125 ◽  
Author(s):  
Lute-Harm Zwiers ◽  
Maarten A De Waard
Genomics ◽  
2013 ◽  
Vol 101 (6) ◽  
pp. 354-361 ◽  
Author(s):  
Yongnam Kim ◽  
Sook-Young Park ◽  
Dongyoung Kim ◽  
Jaeyoung Choi ◽  
Yong-Hwan Lee ◽  
...  

2018 ◽  
Vol 139 (1-3) ◽  
pp. 325-335 ◽  
Author(s):  
Junji Uchiyama ◽  
Ayako Itagaki ◽  
Haruna Ishikawa ◽  
Yu Tanaka ◽  
Hidetaka Kohga ◽  
...  

2017 ◽  
Vol 215 (3) ◽  
pp. 1080-1089 ◽  
Author(s):  
Gui Jie Lei ◽  
Kengo Yokosho ◽  
Naoki Yamaji ◽  
Miho Fujii-Kashino ◽  
Jian Feng Ma

2002 ◽  
Vol 46 (12) ◽  
pp. 3900-3906 ◽  
Author(s):  
Lute-Harm Zwiers ◽  
Ioannis Stergiopoulos ◽  
Johannes G. M. Van Nistelrooy ◽  
Maarten A. De Waard

ABSTRACT Laboratory strains of Mycosphaerella graminicola with decreased susceptibilities to the azole antifungal agent cyproconazole showed a multidrug resistance phenotype by exhibiting cross-resistance to an unrelated chemical, cycloheximide or rhodamine 6G, or both. Decreased azole susceptibility was found to be associated with either decreased or increased levels of accumulation of cyproconazole. No specific relationship could be observed between azole susceptibility and the expression of ATP-binding cassette (ABC) transporter genes MgAtr1 to MgAtr5 and the sterol P450 14α-demethylase gene, CYP51. ABC transporter MgAtr1 was identified as a determinant in azole susceptibility since heterologous expression of the protein reduced the azole susceptibility of Saccharomyces cerevisiae and disruption of MgAtr1 in one specific M. graminicola laboratory strain with constitutive MgAtr1 overexpression restored the level of susceptibility to cyproconazole to wild-type levels. However, the level of accumulation in the mutant with an MgAtr1 disruption did not revert to the wild-type level. We propose that variations in azole susceptibility in laboratory strains of M. graminicola are mediated by multiple mechanisms.


2013 ◽  
Vol 354 (2) ◽  
pp. 563-572 ◽  
Author(s):  
Hideo Mitsuishi ◽  
Takako Kato ◽  
Mo Chen ◽  
Li-Yi Cai ◽  
Hideji Yako ◽  
...  

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Jonas Lee ◽  
Janet Yang ◽  
Daniel Zhitnitsky ◽  
Oded Lewinson ◽  
Douglas Rees

2011 ◽  
Vol 47 ◽  
pp. S339-S340
Author(s):  
V. Hlavac ◽  
R. Vaclavikova ◽  
M. Ehrlichova ◽  
I. Hlavata ◽  
V. Pecha ◽  
...  

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