ACandida albicansGenome Project: Cosmid Contigs, Physical Mapping, and Gene Isolation

1997 ◽  
Vol 21 (3) ◽  
pp. 308-314 ◽  
Author(s):  
Evelyn Tait ◽  
Moyra C. Simon ◽  
Susan King ◽  
Alistair J. Brown ◽  
Neil A.R. Gow ◽  
...  
Keyword(s):  
Physical Mapping ◽  
Gene Isolation ◽  
Cosmid Contigs

scholarly journals Detailed Comparative Mapping of Cereal Chromosome Regions Corresponding to the Ph1 Locus in Wheat

Genetics ◽  
1997 ◽  
Vol 147 (2) ◽  
pp. 801-807 ◽  
Author(s):  
Tracie Foote ◽  
Michael Roberts ◽  
Nori Kurata ◽  
Takuji Sasaki ◽  
Graham Moore
Keyword(s):  
Physical Mapping ◽  
Yeast Artificial Chromosome ◽  
Rice Genome ◽  
Rice Chromosome ◽  
Wheat Chromosome ◽  
Artificial Chromosome ◽  
Gene Isolation ◽  
Chromosome 9 ◽  
Ph1 Locus ◽  
Yac Contigs

Detailed physical mapping of markers from rice chromosome 9, and from syntenous (at the genetic level) regions of other cereal genomes, has resulted in rice yeast artificial chromosome (YAC) contigs spanning parts of rice 9. This physical mapping, together with comparative genetic mapping, has demonstrated that synteny has been largely maintained between the genomes of several cereals at the level of contiged YACs. Markers located in one region of rice chromosome 9 encompassed by the YAC contigs have exhibited restriction fragment length polymorphism (RFLP) using deletion lines for the Ph1 locus. This has allowed demarcation of the region of rice chromosome 9 syntenous with the ph1b and ph1c deletions in wheat chromosome 5B. A group of probes located in wheat homoeologous group 5 and barley chromosome 5H, however, have synteny with rice chromosomes other than 9. This suggests that the usefulness of comparative trait analysis and of the rice genome as a tool to facilitate gene isolation will differ from one region to the next, and implies that the rice genome is more ancestral in structure than those of the Triticeae.

The porcine proteasome subunit A4 (PSMA4 ) gene: isolation of a partial cDNA, linkage and physical mapping

1998 ◽  
Vol 29 (5) ◽  
pp. 385-388 ◽  
Author(s):  
R. Davoli ◽  
L. Fontanesi ◽  
V. Russo ◽  
S. Čepica ◽  
P. Musilová ◽  
...  
Keyword(s):  
Physical Mapping ◽  
Gene Isolation ◽  
Proteasome Subunit ◽  
Partial Cdna

Exosomes: Structure, Biogenesis, Types and Application in Diagnosis and Gene and Drug Delivery

2020 ◽  
Vol 20 (3) ◽  
pp. 195-206 ◽  
Author(s):  
Shriya Agarwal ◽  
Vinayak Agarwal ◽  
Mugdha Agarwal ◽  
Manisha Singh
Keyword(s):  
Drug Delivery ◽  
Gene Therapy ◽  
Immune Responses ◽  
Biological Membrane ◽  
Gene Isolation ◽  
Delivery Vehicle ◽  
Scientific Communities ◽  
Therapeutic Regime ◽  
Targeted Gene Therapy ◽  
Delivery Mechanism

Abstract: In recent times, several approaches for targeted gene therapy (GT) had been studied. However, the emergence of extracellular vesicles (EVs) as a shuttle carrying genetic information between cells has gained a lot of interest in scientific communities. Owing to their higher capabilities in dealing with short sequences of nucleic acid (mRNA, miRNA), proteins, recombinant proteins, exosomes, the most popular form of EVs are viewed as reliable biological therapeutic conveyers. They have natural access through every biological membrane and can be employed for site-specific and efficient drug delivery without eliciting any immune responses hence, qualifying as an ideal delivery vehicle. Also, there are many research studies conducted in the last few decades on using exosome-mediated gene therapy into developing an effective therapy with the concept of a higher degree of precision in gene isolation, purification and delivery mechanism loading, delivery and targeting protocols. This review discusses several facets that contribute towards developing an efficient therapeutic regime for gene therapy, highlighting limitations and drawbacks associated with current GT and suggested therapeutic regimes.

scholarly journals Unusually High Recombination Rate Detected in the Sex Locus Region of the Honey Bee (Apis mellifera)

Genetics ◽  
1999 ◽  
Vol 153 (4) ◽  
pp. 1701-1708 ◽  
Author(s):  
Martin Beye ◽  
Greg J Hunt ◽  
Robert E Page ◽  
M Kim Fondrk ◽  
Lore Grohmann ◽  
...  
Keyword(s):  
Sex Determination ◽  
Physical Mapping ◽  
Recombination Rate ◽  
Heterozygote Advantage ◽  
Range Restriction ◽  
Group Iii ◽  
Single Sex ◽  
Complementary Sex Determination ◽  
Sex Locus ◽  
Map Distance

Abstract Sex determination in Hymenoptera is controlled by haplo-diploidy in which unfertilized eggs develop into fertile haploid males. A single sex determination locus with several complementary alleles was proposed for Hymenoptera [so-called complementary sex determination (CSD)]. Heterozygotes at the sex determination locus are normal, fertile females, whereas diploid zygotes that are homozygous develop into sterile males. This results in a strong heterozygote advantage, and the sex locus exhibits extreme polymorphism maintained by overdominant selection. We characterized the sex-determining region by genetic linkage and physical mapping analyses. Detailed linkage and physical mapping studies showed that the recombination rate is <44 kb/cM in the sex-determining region. Comparing genetic map distance along the linkage group III in three crosses revealed a large marker gap in the sex-determining region, suggesting that the recombination rate is high. We suggest that a “hotspot” for recombination has resulted here because of selection for combining favorable genotypes, and perhaps as a result of selection against deleterious mutations. The mapping data, based on long-range restriction mapping, suggest that the Q DNA-marker is within 20,000 bp of the sex locus, which should accelerate molecular analyses.

Comparative study on the physical mapping of ribosomal genes and repetitive sequences in Friesella schrottkyi (Friese, 1900) (Hymenoptera: Apidae, Meliponini)

2021 ◽  
Author(s):  
Arthur Mayrink Elizeu ◽  
Natalia Martins Travenzoli ◽  
Riudo de Paiva Ferreira ◽  
Denilce Meneses Lopes ◽  
Mara Garcia Tavares
Keyword(s):  
Comparative Study ◽  
Physical Mapping ◽  
Repetitive Sequences ◽  
Ribosomal Genes ◽  
Friesella Schrottkyi
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