Isolation and Characterization of Americanin, a Specific Inhibitor of Thrombin, from the Salivary Glands of the Lone Star TickAmblyomma americanum(L.)

1997 ◽  
Vol 87 (1) ◽  
pp. 30-38 ◽  
Author(s):  
Kuichun Zhu ◽  
Alan S. Bowman ◽  
David L. Brigham ◽  
Richard C. Essenberg ◽  
Jack W. Dillwith ◽  
...  
2008 ◽  
Vol 17 (3) ◽  
pp. 509-518 ◽  
Author(s):  
Nicole Rotter ◽  
Jessica Oder ◽  
Peter Schlenke ◽  
Ulrich Lindner ◽  
Florian Böhrnsen ◽  
...  

1995 ◽  
Vol 19 (2) ◽  
pp. 79-92 ◽  
Author(s):  
A. M. Joubert ◽  
J. C. Crause ◽  
A. R. M. D. Gaspar ◽  
F. C. Clarke ◽  
A. M. Spickett ◽  
...  

1975 ◽  
Vol 4 (4) ◽  
pp. 243-255 ◽  
Author(s):  
Benjamin Paz Aliaga ◽  
Manuel Troyano Pueyo ◽  
Percy Noriega Ponce ◽  
F.J.S. Lara

1993 ◽  
Vol 211 (1-2) ◽  
pp. 317-327 ◽  
Author(s):  
Masaaki TAMURA ◽  
Thomas M. HARRIS ◽  
Fumiko KONISHI ◽  
Tadashi INAGAMI

1977 ◽  
Vol 165 (1) ◽  
pp. 27-32 ◽  
Author(s):  
A R Ashton ◽  
G M Polya

1.3′:5′-Cyclic AMP was extensively purified from Kalanchoe daigremontiana and Agave americana by neutral alumina and anion- and cation-exchange column chromatography. Inclusion of 3′:5′-cyclic [8-3H]AMP from the point of tissue extraction permitted calculation of yields. The purification procedure removed contaminating material that was shown to interfere with the 3′:5′-cyclic AMP estimation and characterization procedures. 2. The partially purified 3′:5′-cyclic AMP was quantified by means of a radiochemical saturation assay using an ox heart 3′:5′-cyclic AMP-binding protein and by an assay involving activation of a mammalian protein kinase. 3. The plant 3′:5′-cyclic AMP co-migrated with 3′:5′-cyclic [8-3H]AMP on cellulose chromatography, poly(ethyleneimine)-cellulose chromatography and silica-gel t.l.c. developed with several solvent systems. 4. The plant 3′:5′-cyclic AMP was degraded by ox heart 3′:5′-cyclic nucleotide phosphodiesterase at the same rates as authentic 3′:5′-cyclic AMP. 1-Methyl-3-isobutylxanthine (1 mM), a specific inhibitor of the 3′:5′-cyclic nucleotide phosphodieterase, completely inhibited such degradation. 5. The concentrations of 3′:5′-cyclic AMP satisfying the above criteria in Kalanchoe and Agave were 2-6 and 1 pmol/g fresh wt. respectively. Possible bacterial contribution to these analyses was estimated to be less than 0.002pmol/g fresh wt. Evidence for the occurrence of 3′:5′-cyclic AMP in plants is discussed.


2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  

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