Plasmodium: A Quantitative Molecular Assay for Detection of Sporogonic-Stage Malaria Parasites

1995 ◽  
Vol 81 (4) ◽  
pp. 436-444 ◽  
Author(s):  
K.D. Vernick ◽  
C. Barreau ◽  
D.C. Seeley
Keyword(s):  
Molecular Assay ◽  
Malaria Parasites ◽  
Sporogonic Stage

scholarly journals Design and Activity of Antimicrobial Peptides against Sporogonic-Stage Parasites Causing Murine Malarias

2002 ◽  
Vol 46 (7) ◽  
pp. 2104-2110 ◽  
Author(s):  
Romanico B. G. Arrighi ◽  
Chikashi Nakamura ◽  
Jun Miyake ◽  
Hilary Hurd ◽  
J. Grant Burgess
Keyword(s):  
Intensity Of Infection ◽  
Malaria Parasites ◽  
Sporogonic Stage ◽  
Short Novel ◽  
Large Numbers ◽  
Level Of Activity ◽  
Parasite Stage ◽  
Pathogenic Microbes

ABSTRACT Insects produce several types of peptides to combat a broad spectrum of invasive pathogenic microbes, including protozoans. However, despite this defense response, infections are often established. Our aim was to design novel peptides that produce high rates of mortality among protozoa of the genus Plasmodium, the malaria parasites. Using existing antimicrobial peptide sequences as templates, we designed and synthesized three short novel hybrids, designated Vida1 to Vida3. Each has a slightly different predicted secondary structure. The peptides were tested against sporogonic stages of the rodent malaria parasites Plasmodium berghei (in vitro and in vivo) and P. yoelii nigeriensis (in vitro). The level of activity varied for each peptide and according to the parasite stage targeted. Vida3 (which is predicted to have large numbers of β sheets and coils but no α helices) showed the highest level of activity, killing the early sporogonic stages in culture and causing highly significant reductions in the prevalence and intensity of infection of P. berghei after oral administration or injection in Anopheles gambiae mosquitoes. The secondary structures of these peptides may play a crucial role in their ability to interact with and kill sporogonic forms of the malaria parasite.

212: Plasma DNA Based Detection of Renal Cell Carcinoma by using New Multiplex Molecular Assay

2007 ◽  
Vol 177 (4S) ◽  
pp. 71-71
Author(s):  
Woo Chul Moon ◽  
C. Noh ◽  
T. Kim ◽  
Sy Oh ◽  
J. Shin ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Molecular Assay ◽  
Plasma Dna

Aceon, Invader UGT1A1 Molecular Assay

2005 ◽  
Vol 35 (19) ◽  
pp. 22
Author(s):  
ELIZABETH MECHCATIE
Keyword(s):  
Molecular Assay
Sign in / Sign up

Export Citation Format

Share Document