Schistosoma mansoni: Genetic Complementation Analysis Shows That Two Independent Hycanthone/Oxamniquine-Resistant Strains Are Mutated in the Same Gene

1993 ◽  
Vol 77 (4) ◽  
pp. 445-449 ◽  
Author(s):  
L. Picamattoccia ◽  
L.C.D. Dias ◽  
R. Moroni ◽  
D. Cioli
Keyword(s):  
Schistosoma Mansoni ◽  
Complementation Analysis ◽  
Genetic Complementation ◽  
Resistant Strains

Interactions between the plasma proteins of Biomphalaria glabrata (Gastropoda) and the sporocyst tegument of Schistosoma mansoni (Trematoda)

Parasitology ◽  
1986 ◽  
Vol 92 (3) ◽  
pp. 653-664 ◽  
Author(s):  
C. J. Bayne ◽  
E. S. Loker ◽  
Mary A. Yui
Keyword(s):  
Schistosoma Mansoni ◽  
Plasma Proteins ◽  
Biomphalaria Glabrata ◽  
Rabbit Antibody ◽  
Tegumental Surface ◽  
Immunological Interactions ◽  
Resistant Strains ◽  
Host Parasite ◽  
Susceptibility And Resistance

SUMMARYThe tegumental surface of Schistosoma mansoni sporocysts is the site of both nutritive and immunological interactions with haemolymph cells and plasma of Biomphalaria glabrata, the schistosome intermediate host. Within minutes of being placed in host plasma, sporocysts acquire plasma antigens, and within 3 h host plasma antigens are present on the surface at near steady state. Though a wide variety of peptides is acquired, there is selection. Furthermore, some differences occur in the peptides acquired from the plasma of susceptible and resistant strains of snail. Acquired antigens are rapidly processed, and are predominantly undetectable in tegumental extracts after a few hours. In contrast, rabbit antibody on sporocysts remains in situ for at least 48 h, so under some conditions there is stable expression of certain tegumental antigenic determinants.These data, obtained using antibodies to snail plasma antigens and to sporocyst tegumental antigens, are discussed in the light of current ideas on the cellular and molecular basis of susceptibility and resistance in this host#parasite system.

Genetic Complementation Analysis of Xeroderma Pigmentosum

1975 ◽  
pp. 725-728 ◽  
Author(s):  
D. Bootsma ◽  
E. A. De Weerd-Kastelein ◽  
W. J. Kleijer ◽  
W. Keÿzez
Keyword(s):  
Xeroderma Pigmentosum ◽  
Complementation Analysis ◽  
Genetic Complementation

scholarly journals QUANTIFICATION OF THE POPULATION AND PHAGOCYTARY ACTIVITY OF HEMOCYTES OF RESISTANT AND SUSCEPTIBLE STRAINS OF Biomphalaria glabrata AND Biomphalaria tenagophila INFECTED WITH Schistosoma mansoni

1997 ◽  
Vol 39 (4) ◽  
pp. 197-202 ◽  
Author(s):  
F. S. de M. BEZERRA ◽  
J. A. NOGUEIRA-MACHADO ◽  
M. M. CHAVES ◽  
R. L. MARTINS ◽  
P. M. Z. COELHO
Keyword(s):  
Schistosoma Mansoni ◽  
Cell Population ◽  
Metabolic Activity ◽  
Post Infection ◽  
Biomphalaria Glabrata ◽  
Determinant Factors ◽  
Circulating Cells ◽  
Resistant Strains ◽  
First Time

Among the determinant factors in the resistance and susceptibility of Biomphalaria to Schistosoma mansoni, hemocytes play an important role. Aiming at studying S. mansoni/Biomphalaria interactions related to hemocytes, the first step is certainly connected with the standardization of this cell population in uninfected Biomphalaria. In this way, quantification of this cell population in hemolymph, as well as its phagocitary capacity, have been determined for the first time. Furthermore, using susceptible and resistant strains of B. glabrata and B. tenagophila, the hemocytegram and phagocytary capacity of hemocytes after infection with S. mansoni were determined too. Resistant and susceptible strains of B.glabrata (BA and BH, respectively), as well as resistant and susceptible strains of B. tenagophila (Taim and CF, respectively) were infected with 10 miracidia of the LE and SJ strains of S. mansoni, respectively. These infected snails and respective uninfected controls were assessed in relation to the number of circulating hemocytes and alteration in the phagocytary capacity, by using Zymozan and MTT. Reading was taken by means of a spectrophotometer at 5 hours and 1,2,5,10,20 and 30 days after infection. The results showed a decrease in population of the circulating phagocytary cells, 5 hours after infection. One day post-infection, the circulating cells of the susceptible snails showed an increased metabolic activity, but the same event could not be observed in the resistant strains. In the subsequent observation periods, significant differences among the strains studied could not be observed until the end of the experiment

scholarly journals Strain variation in the infectivity of Schistosoma mansoni for Biomphalaria glabrata

1988 ◽  
Vol 30 (2) ◽  
pp. 86-90 ◽  
Author(s):  
Luiz Candido de Souza Dias ◽  
John I. Bruce ◽  
Gerald C. Coles
Keyword(s):  
Schistosoma Mansoni ◽  
Puerto Rican ◽  
Biomphalaria Glabrata ◽  
The Other ◽  
Drug Resistant ◽  
Strain Variation ◽  
Resistant Strains ◽  
Drug Resistant Strains

Five strains of Schistosoma mansoni resistant and susceptible to schistosomicides were studied for infectivity of 2 strains of Biomphalaria glabrata one of Puerto Rican origin and the other of Brazilian origin. Puerto Rican strains of S. Mansoni developed more slowly and had a lower infectivity in Brazilian B. glabrata than did the Brazilian S. mansoni. However, Brazilian S. Mansoni developed as well in Puerto Rican snails as in Brazilian snails, indicating that drug resistant strains could easily be moved by travel of infected persons from one area to another.

Genetic complementation analysis of rice sucrose transporter genes in Arabidopsis SUC2 mutant atsuc2

2016 ◽  
Vol 59 (3) ◽  
pp. 231-237 ◽  
Author(s):  
Joon-Seob Eom ◽  
Cong Danh Nguyen ◽  
Dae-Woo Lee ◽  
Sang-Kyu Lee ◽  
Jong-Seong Jeon
Keyword(s):  
Complementation Analysis ◽  
Genetic Complementation ◽  
Sucrose Transporter

scholarly journals Structural, functional and docking analysis against Schistosoma mansoni dihydroorotate dehydrogenase for potential chemotherapeutic drugs

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 651 ◽  
Author(s):  
Benson Otarigho
Keyword(s):  
Schistosoma Mansoni ◽  
Chemotherapeutic Drugs ◽  
Dihydroorotate Dehydrogenase ◽  
Computational Tools ◽  
Docking Analysis ◽  
Computational Approaches ◽  
Intermolecular Energy ◽  
Resistant Strains ◽  
Approved Drugs ◽  
Free Energy Of Binding

Background: Praziquantel, as the only drug for the treatment of schistosomiasis, is under serious threat due to the emergence of resistant strains of Schistosoma species. There is an urgent need to search for alternative chemotherapy to supplement or complement praziquantel. Schistosoma dihydroorotate dehydrogenase (DHODH) has been recommended as a druggable target for schistosomiasis chemotherapy. The development of novel molecular modeling approaches, alongside with computational tools and rapid sequencing of pathogen genomes, have facilitated drug discovery. Therefore, the aim of this study was to employ computational approaches to screen compounds against Schistosoma mansoni DHODH. Methods: In this study, DHODH was used to blast on the latest version of DrugBank that contained 12,110 compounds, resulting in 26 drugs that can bind. Results: In silico docking shows that 13 drugs can bind strongly with an estimated free energy of binding, total intermolecular energy and estimated inhibition constant (Ki) greater than or equal to -8.6 kcal/mol, -8.12 kcal/mol and 1.12 µM, respectively. These compounds include the approved drugs manitimus, capecitabine, brequinar analog and leflunomide. Conclusions: These results indicate that these drugs have the potential for use in the control of schistosomiasis in the future.

scholarly journals Genetic complementation analysis of Escherichia coli type 1 somatic pilus mutants.

1977 ◽  
Vol 130 (1) ◽  
pp. 506-511 ◽  
Author(s):  
L M Swaney ◽  
Y P Liu ◽  
K Ippen-Ihler ◽  
C C Brinton
Keyword(s):  
Escherichia Coli ◽  
Complementation Analysis ◽  
Genetic Complementation
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