Demonstration of α-Synuclein Immunoreactivity in Neuronal and Glial Cytoplasm in Normal Human Brain Tissue Using Proteinase K and Formic Acid Pretreatment

2002 ◽  
Vol 176 (1) ◽  
pp. 98-104 ◽  
Author(s):  
Fumiaki Mori ◽  
Kunikazu Tanji ◽  
Makoto Yoshimoto ◽  
Hitoshi Takahashi ◽  
Koichi Wakabayashi
Keyword(s):  
Human Brain ◽  
Formic Acid ◽  
Brain Tissue ◽  
Proteinase K ◽  
Human Brain Tissue ◽  
Acid Pretreatment ◽  
Normal Human Brain ◽  
Normal Human

scholarly journals Gray and white matter metabolite differences in Alzheimer’s disease and normal human brain tissue

2020 ◽  
Vol 16 (S2) ◽  
Author(s):  
Tyler C. Hammond ◽  
Xin Xing ◽  
Peter T. Nelson ◽  
Stephanie Ham ◽  
Ai‐Ling Lin
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
White Matter ◽  
Human Brain ◽  
Brain Tissue ◽  
Human Brain Tissue ◽  
Normal Human Brain ◽  
Normal Human

Detection of Borna disease virus genome in normal human brain tissue

1997 ◽  
Vol 770 (1-2) ◽  
pp. 307-309 ◽  
Author(s):  
Seiichi Haga ◽  
Masahiro Yoshimura ◽  
Yumiko Motoi ◽  
Kunimasa Arima ◽  
Takako Aizawa ◽  
...  
Keyword(s):  
Human Brain ◽  
Brain Tissue ◽  
Disease Virus ◽  
Virus Genome ◽  
Human Brain Tissue ◽  
Borna Disease Virus ◽  
Normal Human Brain ◽  
Normal Human ◽  
Borna Disease

Human Herpesvirus 6 Infection in Normal Human Brain Tissue

1994 ◽  
Vol 169 (4) ◽  
pp. 943-944 ◽  
Author(s):  
M. Luppi ◽  
P. Barozzi ◽  
A. Maiorana ◽  
R. Marasca ◽  
G. Torelli
Keyword(s):  
Human Brain ◽  
Brain Tissue ◽  
Human Herpesvirus ◽  
Human Herpesvirus 6 ◽  
Human Brain Tissue ◽  
Normal Human Brain ◽  
Normal Human ◽  
Herpesvirus 6

scholarly journals Neuronal localization of GAS7 within human brain tissue: Implications for schizophrenia research

2018 ◽  
Vol 10 (4) ◽  
Author(s):  
Michael A. Meyer
Keyword(s):  
Cerebral Cortex ◽  
Human Brain ◽  
Brain Tissue ◽  
Granule Cells ◽  
Molecular Data ◽  
Histochemical Localization ◽  
Human Brain Tissue ◽  
Sequencing Studies ◽  
Normal Human ◽  
Molecular Layers

In view of recent data on the linkage of Gas7 protein to schizophrenia, and in view of its role in neurite outgrowth, histochemical localization of the Gas7 protein was studied in normal human brain tissue using an online tissue atlas. Selective localization to neurons in the cerebral cortex was found with moderate levels in the hippocampus and caudate, but fairly low levels were noted within the human cerebellum and was limited to small granule cells as well as the neuropil of the cerebellar molecular layers. Despite this low intensity histochemical localization in the cerebellum, molecular data indicate a substantially large number of RNA transcripts in the cerebellum that exceeded the cerebral cortex as determined by sequencing studies.

scholarly journals RBIO-06. IMPLEMENTATION OF HUMAN INDUCED PLURIPOTENT STEM CELL DERIVED CEREBRAL ORGANOIDS TO MODEL NORMAL TISSUE RADIORESPONSE

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii193-ii193
Author(s):  
Lawrence Bronk ◽  
Sanjay Singh ◽  
Riya Thomas ◽  
Luke Parkitny ◽  
Mirjana Maletic-Savatic ◽  
...  
Keyword(s):  
Stem Cell ◽  
Human Brain ◽  
Brain Tissue ◽  
Human Tissue ◽  
Model Systems ◽  
Human Brain Tissue ◽  
Post Irradiation ◽  
Mature Neurons ◽  
Induced Pluripotent ◽  
Effects Of Radiation

Abstract Treatment-related sequelae following cranial irradiation have life changing impacts for patients and their caregivers. Characterization of the basic response of human brain tissue to irradiation has been difficult due to a lack of preclinical models. The direct study of human brain tissue in vitro is becoming possible due to advances in stem cell biology, neuroscience, and tissue engineering with the development of organoids as novel model systems which enable experimentation with human tissue models. We sought to establish a cerebral organoid (CO) model to study the radioresponse of normal human brain tissue. COs were grown using human induced pluripotent stem cells and a modified Lancaster protocol. Compositional analysis during development of the COs showed expected populations of neurons and glia. We confirmed a population of microglia-like cells within the model positive for the makers Iba1 and CD68. After 2-months of maturation, COs were irradiated to 0, 10, and 20 Gy using a Shepard Mark-II Cs-137 irradiator and returned to culture. Subsets of COs were prepared for immunostaining at 30- and 70-days post-irradiation. To examine the effect of irradiation on the neural stem cell (NSC) population, sections were stained for SOX2 and Ki-67 expression denoting NSCs and proliferation respectively. Slides were imaged and scored using the CellProfiler software package. The percentage of proliferating NSCs 30-days post-irradiation was found to be significantly reduced for irradiated COs (5.7% (P=0.007) and 3.4% (P=0.001) for 10 and 20 Gy respectively) compared to control (12.7%). The reduction in the proliferating NSC population subsequently translated to a reduced population of NeuN-labeled mature neurons 70 days post-irradiation. The loss of proliferating NSCs and subsequent reduction in mature neurons demonstrates the long-term effects of radiation. Our initial results indicate COs will be a valuable model to study the effects of radiation therapy on normal and diseased human tissue.

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