Stable Expression of the Alkaline Phosphatase Marker Gene by Neural Cells in Culture and after Transplantation into the CNS Using Cells Derived from a Transgenic Rat

2002 ◽  
Vol 174 (1) ◽  
pp. 48-57 ◽  
Author(s):  
Tahmina Mujtaba ◽  
Steve S.W. Han ◽  
Itzhak Fischer ◽  
Eric P. Sandgren ◽  
Mahendra S. Rao
Keyword(s):  
Alkaline Phosphatase ◽  
Marker Gene ◽  
Stable Expression ◽  
Transgenic Rat ◽  
Neural Cells ◽  
Cells In Culture

scholarly journals Remyelination of spinal cord axons by olfactory ensheathing cells and Schwann cells derived from a transgenic rat expressing alkaline phosphatase marker gene

2004 ◽  
Vol 1 (1) ◽  
pp. 47-55 ◽  
Author(s):  
YUKINORI AKIYAMA ◽  
KAREN LANKFORD ◽  
CHRISTINE RADTKE ◽  
CHARLES A. GREER ◽  
JEFFERY D. KOCSIS
Keyword(s):  
Spinal Cord ◽  
Alkaline Phosphatase ◽  
Marker Gene ◽  
Cell Suspensions ◽  
Olfactory Ensheathing Cells ◽  
Transgenic Rat ◽  
Placental Alkaline Phosphatase ◽  
Fiber Layer ◽  
Ensheathing Cells

Transplantation of cell suspensions containing olfactory ensheathing cells (OECs) has been reported to remyelinate demyelinated axons in the spinal cord with a Schwann cell (SC)-like pattern of myelination. However, questions have been raised recently as to whether OECs can form SC-like myelin. To address this issue we prepared SCs and OECs from transgenic rats in which a marker gene, human placental alkaline phosphatase (hPAP), is linked to the ubiquitously active promoter of the R26 gene. SCs were prepared from the sciatic nerve and OECs from the outer nerve-fiber layer of the olfactory bulb. Positive S100 and p75 immunostaining indicated that >95% of cells in culture displayed either SC or OEC phenotypes. Suspensions of either SCs or OECs were transplanted into an X-irradiation/ethidium bromide demyelinating lesion in the spinal cord. We observed extensive SC-like remyelination following either SC or OEC transplantation 3 weeks after injection of the cells. Alkaline phosphatase (ALP) chromagen reaction product was associated clearly with the myelin-forming cells. Thus, cell suspensions that are enriched in either SCs or OECs result in peripheral-like myelin when transplanted in vivo.

scholarly journals miR-539 mediates osteoblast mineralization by regulating Distal-less genes 2 in MC3T3-E1 cell line

2017 ◽  
Vol 12 (1) ◽  
pp. 294-299 ◽  
Author(s):  
Jianguo Han ◽  
Li Su ◽  
Chunyang Zhang ◽  
Rongcai Jiang
Keyword(s):  
Gene Expression ◽  
Alkaline Phosphatase ◽  
Cell Line ◽  
Osteoblast Differentiation ◽  
Marker Gene ◽  
Inhibitory Effect ◽  
Negative Regulator ◽  
Expression Level ◽  
Matrix Mineralization ◽  
Marker Gene Expression

AbstractmicroRNAs (miRNAs) play an important role in osteoblast differentiation. However, the mechanisms of miRNAs regulating osteoblast mineralization still needs to be further cleared. Distal-less genes 2 (Dlx2) plays an important role in osteoblast differentiation. We have found that miR-539 was significantly downregulated and Dlx2 was found to be inversely correlated with miR-539 in MC3T3-E1 cell line during osteoblast mineralization. The overexpression of miR-539 significantly decreased the expression level of Dlx2 and suppressed the osteogenic marker gene expression level, alkaline phosphatase activity and matrix mineralization. Our study showed that miR-539 was a negative regulator in osteoblast mineralization and that the targeting of Dlx2 gene partly contributes to this inhibitory effect exerted by miR-539.

T cells show preferential adhesion to enteric neural cells in culture and are close to neural cells in the myenteric ganglia of Crohn's patients

2020 ◽  
Vol 349 ◽  
pp. 577422
Author(s):  
Julie Pabois ◽  
Tony Durand ◽  
Catherine Le Berre ◽  
Jacques Gonzales ◽  
Michel Neunlist ◽  
...  
Keyword(s):  
T Cells ◽  
Neural Cells ◽  
Cells In Culture ◽  
Myenteric Ganglia

Increased extracellular magnesium modulates proliferation in fetal neural cells in culture

2001 ◽  
Vol 127 (2) ◽  
pp. 99-109 ◽  
Author(s):  
Richard C Krueger ◽  
Matthew T Santore ◽  
Glyn Dawson ◽  
Nancy B Schwartz
Keyword(s):  
Neural Cells ◽  
Cells In Culture
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