Intracerebroventricular Glial Cell Line-Derived Neurotrophic Factor Improves Motor Function and Supports Nigrostriatal Dopamine Neurons in Bilaterally 6-Hydroxydopamine Lesioned Rats

1997 ◽  
Vol 145 (1) ◽  
pp. 104-117 ◽  
Author(s):  
Kathryn E. Bowenkamp ◽  
Paul A. Lapchak ◽  
Barry J. Hoffer ◽  
Paul J. Miller ◽  
Paula C. Bickford
Keyword(s):  
Cell Line ◽  
Glial Cell ◽  
Motor Function ◽  
Neurotrophic Factor ◽  
Dopamine Neurons ◽  
Nigrostriatal Dopamine ◽  
6 Hydroxydopamine ◽  
Nigrostriatal Dopamine Neurons

scholarly journals Enhancement of graft survival and sensorimotor behavioral recovery in rats undergoing transplantation with dopaminergic cells exposed to glial cell line-derived neurotrophic factor

1999 ◽  
Vol 7 (3) ◽  
pp. E6
Author(s):  
Vivek Mehta ◽  
Murray Hong ◽  
Julian Spears ◽  
Ivar Mendez
Keyword(s):  
Cell Line ◽  
Glial Cell ◽  
Neurotrophic Factor ◽  
Fetal Tissue ◽  
Cell Number ◽  
Genetically Engineered ◽  
Nigrostriatal Pathway ◽  
Behavioral Recovery ◽  
Additional Improvement ◽  
6 Hydroxydopamine

The goal of this study was to investigate the ability of fetal dopaminergic neurons to improve complex sensorimotor behavior. The authors obtained ventral mesencephalic tissue from 14-day-old rat fetuses. The cells were exposed to glial cell line-derived neurotrophic factor (GDNF) prior to transplantation into rats with unilateral 6-hydroxydopamine lesions of the dopaminergic nigrostriatal pathway. Animals that received 400,000 cells exposed to GDNF demonstrated significant improvement in contralateral forelimb function and showed improvement in rotational behavior faster than animals that received cells not exposed to GDNF. Increasing the number of implanted cells to 800,000 exposed to GDNF did not result in any additional improvement in functional recovery. As neural grafting procedures in the nervous system evolve and genetically engineered cells or stem cells replace fetal tissue, crucial questions about cell number and trophic regulation will need to be addressed. This study demonstrates that grafting of 400,000 cells exposed to GDNF before transplantation has a beneficial effect in the restoration of complex sensorimotor behavior.

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