Suppressive Effects of Thyroxine on Glucocorticoid (GC)-induced Metabolic Changes and Cataract Formation on Developing Chick Embryos

2001 ◽  
Vol 72 (6) ◽  
pp. 643-648 ◽  
Author(s):  
Hiroshi Kosano ◽  
Hiroshi Watanabe ◽  
Hideo Nishigori
Keyword(s):  
Metabolic Changes ◽  
Chick Embryos ◽  
Cataract Formation

scholarly journals Steroid cataract in patients with hemoblastosis

2019 ◽  
Vol 18 (2) ◽  
pp. 114-119
Author(s):  
A. B. Smirnova ◽  
B. S. Pershin ◽  
N. V. Myakova
Keyword(s):  
Aqueous Humor ◽  
Radiation Treatment ◽  
Life Quality ◽  
Metabolic Changes ◽  
Cataract Formation ◽  
Treatment Results ◽  
Biochemical Studies ◽  
Physiological And Biochemical ◽  
Modern Technologies

Modern technologies of treatment of children with oncohaematological diseases allowed to noticebly increase the survival indexes in this group of patients, enhancing the value of maintenance of their life quality. More than half of those who received long-term steriod and radiation treatment develop cataract that causes decrease in vision. In this review we represent data concerning mechanisms of cataract formation in patients after steriod and radiation treatment, results of anatomical, physiological and biochemical studies of the lens as well as metabolic changes in aqueous humor leading to cataract formation.

Nitrogen Excretion in Developing Chick Embryos

Development ◽  
1957 ◽  
Vol 5 (3) ◽  
pp. 215-224
Author(s):  
James R. Fisher ◽  
Robert E. Eakin
Keyword(s):  
Uric Acid ◽  
Experimental Evidence ◽  
Nitrogen Metabolism ◽  
Metabolic Changes ◽  
Nitrogen Excretion ◽  
Chick Embryos ◽  
The Many

Of the many examples of metabolic changes occurring during development, the most widely cited is that in developing chicks wherein the end product of nitrogen metabolism has been assumed to shift from ammonia to urea and finally to uric acid (Needham, 1931). This system appeared to be ideal for a proposed study of the mechanisms bringing about such changes in metabolism, providing the occurrence of these changes could be further substantiated. The experimental evidence upon which this pattern had been postulated consisted entirely of measurements of the total amounts of ammonia, urea, and uric acid in the allantois and the ratio of their total weights to the weight of the embryo. Nothing was reported concerning the changes in concentration of these substances nor their presence outside the allantois. Lack of this information makes it impossible to conclude that net synthesis occurs, particularly in the cases of ammonia and urea where the total amounts present are quite small.

Alteration of hepatic lipidperoxide levels during cataract formation caused by glucocorticoids in developing chick embryos

Life Sciences ◽  
1984 ◽  
Vol 35 (9) ◽  
pp. 981-985 ◽  
Author(s):  
Hideo Nishigori ◽  
Jung W. Lee ◽  
Yoshiko Iwamoto ◽  
Rume Hayashi ◽  
Kazuo Maruyama ◽  
...  
Keyword(s):  
Chick Embryos ◽  
Cataract Formation

Preventive effect of ascorbic acid against glucocorticoid-induced cataract formation of developing chick embryos

1985 ◽  
Vol 40 (3) ◽  
pp. 445-451 ◽  
Author(s):  
Hideo Nishigori ◽  
Rume Hayashi ◽  
Jung W. Lee ◽  
Kazuo Maruyama ◽  
Motoharu Iwatsuru
Keyword(s):  
Ascorbic Acid ◽  
Chick Embryos ◽  
Cataract Formation ◽  
Preventive Effect

Dexamethasone Effect on Embryonic Chick Respiratory Epithelium

1982 ◽  
Vol 40 ◽  
pp. 248-249
Author(s):  
M.R. Richter ◽  
R.V. Blystone
Keyword(s):  
Lung Development ◽  
Critical Role ◽  
Respiratory Epithelium ◽  
Chick Embryos ◽  
Embryonic Chick ◽  
White Leghorn ◽  
Lung Maturation ◽  
Synthetic Analogs ◽  
Lung Physiology ◽  
Avian Lung

Dexamethasone and other synthetic analogs of corticosteroids have been employed clinically as enhancers of lung development. The mechanism(s) by which this steroid induction of later lung maturation operates is not clear. This study reports the effect on lung epithelia of dexamethasone administered at different intervals during development. White Leghorn chick embryos were used so as to remove possible maternal and placental influences on the exogenously applied steroid. Avian lung architecture does vary from mammals; however, respiratory surfactant produced by the lung epithelia serves an equally critical role in avian lung physiology.

Development of the Foramen Secundum in the Chick as Observed by Scanning Electron Microscopy

1976 ◽  
Vol 34 ◽  
pp. 212-213
Author(s):  
M.J.C. Hendrix ◽  
D.E. Morse
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Procaine Hydrochloride ◽  
Cardiac Anomaly ◽  
Chick Embryos ◽  
Congenital Cardiac Anomaly ◽  
Septal Defects ◽  
Critical Point Drying ◽  
Scanning Electron

Atrial septal defects are considered the most common congenital cardiac anomaly occurring in humans. In studying the normal sequential development of the atrial septum, chick embryos of the White Leghorn strain were prepared for scanning electron microscopy and the results were then extrapolated to the human heart. One-hundred-eighty chick embryos from 2 to 21 days of age were removed from their shells and immersed in cold cacodylate-buffered aldehyde fixative . Twenty-four embryos through the first week post-hatching were perfused in vivo using cold cacodylate-buffered aldehyde fixative with procaine hydrochloride. The hearts were immediately dissected free and remained in the fixative a minimum of 2 hours. In most cases, the lateral atrial walls were removed during this period. The tissues were then dehydrated using a series of ascending grades of ethanol; final dehydration of the tissues was achieved via the critical point drying method followed by sputter-coating with goldpalladium.

A Cytochemical Study of Glucose-6-Phosphatase (G-6-PASE) in Cells Participating in Atherogenesis of Rabbit Aorta

1975 ◽  
Vol 33 ◽  
pp. 460-461
Author(s):  
Sidney D. Kobernick ◽  
Edna A. Elfont ◽  
Neddra L. Brooks
Keyword(s):  
Lipid Metabolism ◽  
New Zealand ◽  
Aortic Wall ◽  
Rabbit Aorta ◽  
Plaque Formation ◽  
Metabolic Changes ◽  
Atherosclerotic Plaques ◽  
Cytochemical Study ◽  
Cellular Alteration ◽  
New Zealand White Rabbits

This cytochemical study was designed to investigate early metabolic changes in the aortic wall that might lead to or accompany development of atherosclerotic plaques in rabbits. The hypothesis that the primary cellular alteration leading to plaque formation might be due to changes in either carbohydrate or lipid metabolism led to histochemical studies that showed elevation of G-6-Pase in atherosclerotic plaques of rabbit aorta. This observation initiated the present investigation to determine how early in plaque formation and in which cells this change could be observed.Male New Zealand white rabbits of approximately 2000 kg consumed normal diets or diets containing 0.25 or 1.0 gm of cholesterol per day for 10, 50 and 90 days. Aortas were injected jin situ with glutaraldehyde fixative and dissected out. The plaques were identified, isolated, minced and fixed for not more than 10 minutes. Incubation and postfixation proceeded as described by Leskes and co-workers.

Immunocytochemical localization of desmin and vimentin in chick embryonic myocardial cells

1990 ◽  
Vol 48 (3) ◽  
pp. 448-449
Author(s):  
Yukiko Sugi
Keyword(s):  
Sodium Methoxide ◽  
Intermediate Filament Protein ◽  
Chick Embryos ◽  
Immunocytochemical Localization ◽  
Myocardial Cells ◽  
Immunofluorescence Study ◽  
Immunofluorescent Localization ◽  
Rabbit Igg ◽  
Semithin Sections ◽  
Filament Protein

In cultured skeletal muscle cells of chick, one intermediate filament protein, vimentin, is primarily formed and then synthesis of desmin follows. Coexistence of vimentin and desmin has been immunocytochemically confirmed in chick embryonic skeletal musclecells. Immunofluorescent localization of vimentin and desmin has been described in developing myocardial cells of hamster. However, initial localization of desmin and vimentin in early embryonic heart has not been reported in detail. By quick-freeze deep-etch method a loose network of intermediate filaments was revealed to exist surrounding myofibrils. In this report, immunocytochemical localization of desmin and vimentin is visualized in early stages of chick embryonic my ocardium.Chick embryos, Hamburger-Hamilton (H-H) stage 8 to hatch, and 1 day old postnatal chicks were used in this study. For immunofluorescence study, each embryo was fixed with 4% paraformaldehyde and embedded in Epon 812. De-epoxinized with sodium methoxide, semithin sections were stained with primary antibodies (rabbit anti-desmin antibody and anti-vimentin antibody)and secondary antibody (RITC conjugated goat-anti rabbit IgG).

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