Relationship Between Orthogonal Arrays of Particles in the Rat Ciliary Epithelium and Maturation of the Blood-Aqueous Barrier

1998 ◽  
Vol 66 (5) ◽  
pp. 675-680 ◽  
Author(s):  
MICHEL HIRSCH ◽  
WALTER NOSKE ◽  
SORAYA MOULESSEHOUL ◽  
YVES POULIQUEN
Keyword(s):  
Orthogonal Arrays ◽  
Ciliary Epithelium ◽  
Orthogonal Arrays Of Particles ◽  
Blood Aqueous Barrier

scholarly journals Histamine treatment induces rearrangements of orthogonal arrays of particles (OAPs) in human AQP4-expressing gastric cells

2001 ◽  
Vol 154 (6) ◽  
pp. 1235-1244 ◽  
Author(s):  
Monica Carmosino ◽  
Giuseppe Procino ◽  
Grazia Paola Nicchia ◽  
Roberta Mannucci ◽  
Jean-Marc Verbavatz ◽  
...  
Keyword(s):  
Cell Line ◽  
Basolateral Membrane ◽  
Water Channel ◽  
Freeze Fracture ◽  
Orthogonal Arrays ◽  
Short Term ◽  
Water Permeability Coefficient ◽  
Cell Surface Biotinylation ◽  
Osmotic Water ◽  
Orthogonal Arrays Of Particles

To test the involvement of the water channel aquaporin (AQP)-4 in gastric acid physiology, the human gastric cell line (HGT)-1 was stably transfected with rat AQP4. AQP4 was immunolocalized to the basolateral membrane of transfected HGT-1 cells, like in native parietal cells. Expression of AQP4 in transfected cells increased the osmotic water permeability coefficient (Pf) from 2.02 ± 0.3 × 10−4 to 16.37 ± 0.5 × 10−4 cm/s at 20°C. Freeze-fracture EM showed distinct orthogonal arrays of particles (OAPs), the morphological signature of AQP4, on the plasma membrane of AQP4-expressing cells. Quantitative morphometry showed that the density of OAPs was 2.5 ± 0.3% under basal condition and decreased by 50% to 1.2 ± 0.3% after 20 min of histamine stimulation, mainly due to a significant decrease of the OAPs number. Concomitantly, Pf decreased by ∼35% in 20-min histamine-stimulated cells. Both Pf and OAPs density were not modified after 10 min of histamine exposure, time at which the maximal hormonal response is observed. Cell surface biotinylation experiments confirmed that AQP4 is internalized after 20 min of histamine exposure, which may account for the downregulation of water transport. This is the first evidence for short term rearrangement of OAPs in an established AQP4-expressing cell line.

Absence of orthogonal arrays in kidney, brain and muscle from transgenic knockout mice lacking water channel aquaporin-4

1997 ◽  
Vol 110 (22) ◽  
pp. 2855-2860 ◽  
Author(s):  
J.M. Verbavatz ◽  
T. Ma ◽  
R. Gobin ◽  
A.S. Verkman
Keyword(s):  
Cho Cells ◽  
Collecting Duct ◽  
Water Channel ◽  
Freeze Fracture ◽  
Orthogonal Arrays ◽  
Knock Out ◽  
Orthogonal Arrays Of Particles ◽  
Freeze Fracture Electron Microscopy ◽  
Knock Out Mice ◽  
And Function

Freeze-fracture electron microscopy (FFEM) of kidney collecting duct, muscle, astrocytes in brain, and other mammalian tissues has revealed regular square arrays of intramembrane particles called orthogonal arrays of particles (OAPs). Their possible role in membrane structure and transport have been proposed, and their absence or decrease has been noted in a variety of hereditary and acquired diseases. A transgenic mouse lacking water channel AQP4 was used to show that AQP4 is the OAP protein. FFEM was done on kidney, skeletal muscle, and brain from AQP4 wild-type [+/+], heterozygous [+/−] and knock-out [-/-] mice. The [-/-] mice did not express detectable AQP4 protein, but were grossly indistinguishable from [+/+] mice. FFEM was done on blinded samples of kidney, brain and muscle from 9 mice. In all 6 kidney samples from [+/+] and [+/−] mice, OAPs similar to those in AQP4-transfected CHO cells were found in basolateral membranes of collecting duct principal cells. In all muscle and brain samples from [+/+] and [+/−] mice, OAPs of identical ultrastructure to those in kidney were seen, but in smaller patch sizes. OAPs were not seen in any sample from [-/-] mice. Label-fracture analysis using a peptide-derived AQP4 polyclonal antibody showed immunogold labeling of OAPs in AQP4-expressing CHO cells. These studies provide direct evidence that AQP4 is required for formation of OAPs and is a component of OAPs, thus establishing the identity and function of OAPs.

Structure and Functions of Aquaporin-4-Based Orthogonal Arrays of Particles

2011 ◽  
pp. 1-41 ◽  
Author(s):  
Hartwig Wolburg ◽  
Karen Wolburg-Buchholz ◽  
Petra Fallier-Becker ◽  
Susan Noell ◽  
Andreas F. Mack
Keyword(s):  
Orthogonal Arrays ◽  
Aquaporin 4 ◽  
Orthogonal Arrays Of Particles ◽  
Structure And Functions

scholarly journals Aquaporin-4 orthogonal arrays of particles from a physiological and pathophysiological point of view

2013 ◽  
Vol 2 (4) ◽  
pp. 143-154 ◽  
Author(s):  
Grazia Paola Nicchia ◽  
Francesco Pisani ◽  
Angelo Sparaneo ◽  
Maria Grazia Mola ◽  
Davide Basco ◽  
...  
Keyword(s):  
Orthogonal Arrays ◽  
Point Of View ◽  
Aquaporin 4 ◽  
Orthogonal Arrays Of Particles

scholarly journals Is Upregulation of Aquaporin 4-M1 Isoform Responsible for the Loss of Typical Orthogonal Arrays of Particles in Astrocytomas?

2016 ◽  
Vol 17 (8) ◽  
pp. 1230 ◽  
Author(s):  
Petra Fallier-Becker ◽  
Maike Nieser ◽  
Ulrike Wenzel ◽  
Rainer Ritz ◽  
Susan Noell
Keyword(s):  
Orthogonal Arrays ◽  
Aquaporin 4 ◽  
Orthogonal Arrays Of Particles

Agrin defines polarized distribution of orthogonal arrays of particles in astrocytes

2009 ◽  
Vol 337 (2) ◽  
pp. 185-195 ◽  
Author(s):  
Susan Noell ◽  
Petra Fallier-Becker ◽  
Urban Deutsch ◽  
Andreas F. Mack ◽  
Hartwig Wolburg
Keyword(s):  
Orthogonal Arrays ◽  
Orthogonal Arrays Of Particles

scholarly journals Orthogonal Arrays of Particles alter cytoskeleton and cell invasion dynamics in GBM and glioma cells

2018 ◽  
Vol WCP2018 (0) ◽  
pp. OR34-2
Author(s):  
Grazia P. Nicchia ◽  
Laura Simone ◽  
Maria G. Mola ◽  
Francesco Pisani ◽  
Concetta D. Gargano ◽  
...  
Keyword(s):  
Cell Invasion ◽  
Glioma Cells ◽  
Orthogonal Arrays ◽  
Invasion Dynamics ◽  
Orthogonal Arrays Of Particles
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