Immunolocalization of Prolyl 4-Hydroxylase Subunits, α-Smooth Muscle Actin, and Extracellular Matrix Components in Human Lens Capsules with Lens Implants

1998 ◽  
Vol 66 (3) ◽  
pp. 283-294 ◽  
Author(s):  
SHIZUYA SAIKA ◽  
YOSHIJI KAWASHIMA ◽  
TAKESHI MIYAMOTO ◽  
YUKA OKADA ◽  
SAI-ICHI TANAKA ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Human Lens ◽  
Muscle Actin ◽  
Extracellular Matrix Components ◽  
Matrix Components

scholarly journals Extracellular matrix components and regulators in the airway smooth muscle in asthma

2008 ◽  
Vol 32 (1) ◽  
pp. 61-69 ◽  
Author(s):  
B. B. Araujo ◽  
M. Dolhnikoff ◽  
L. F. F. Silva ◽  
J. Elliot ◽  
J. H. N. Lindeman ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Smooth Muscle ◽  
Airway Smooth Muscle ◽  
Extracellular Matrix Components ◽  
Matrix Components

Osteopontin expression in spontaneously developed neointima in fowl (Gallus gallus)

2000 ◽  
Vol 203 (2) ◽  
pp. 273-282
Author(s):  
R.J. Kuykindoll ◽  
H. Nishimura ◽  
D.B. Thomason ◽  
S.K. Nishimoto
Keyword(s):  
Extracellular Matrix ◽  
Smooth Muscle ◽  
Abdominal Aorta ◽  
Smooth Muscle Actin ◽  
Polyacrylamide Gel Electrophoresis ◽  
Balloon Catheter ◽  
Protein Band ◽  
Muscle Actin ◽  
Smooth Muscle Tissue ◽  
Aortic Smooth Muscle

Fowl show spontaneous elevation of blood pressure and neointimal plaque formation in the abdominal aorta at young ages. A similar neointima can be induced by a balloon-catheter-induced endothelium injury to the fowl aorta. Both spontaneously developed and injury-induced vascular lesions exhibit subendothelial hyperplasia consisting of neointimal cells with a synthetic phenotype and abundant extracellular matrix. The role of the extracellular matrix in the formation of neointima is not known. In this study, we investigated whether osteopontin, an adhesive glycoprotein present in the extracellular matrix, is expressed in aortic smooth muscle tissue of the fowl abdominal aorta, in spontaneously developed neointimal plaques and in the aortic smooth muscle underlying neointimal plaques. Crude protein extracted from isolated aortic smooth muscle tissues and neointimal plaques was fractionated by SDS-polyacrylamide gel electrophoresis and analyzed by immunoblotting with rabbit anti-fowl osteopontin (provided by Dr L. C. Gerstenfeld, Boston University) or anti-α smooth muscle actin antibodies. The anti-fowl osteopontin antibody predominantly recognized a 66–70 kDa protein band in neointimal plaques that co-migrated with the osteopontin phosphoprotein from chick bone. In contrast, intact aortic smooth muscle and the smooth muscle underlying neointimal plaques equally expressed three proteins (66–70 kDa, approximately 50 kDa and approximately 43 kDa) recognized by the anti-osteopontin antibody. Anti-α smooth muscle actin antibody recognized a 43 kDa protein band, and the expression of α smooth muscle actin was higher in aortic smooth muscle than in neointimal plaques. Osteopontin mRNA expression was examined using reverse transcription-polymerase chain reaction (RT-PCR) of total RNA from vascular tissues with specific primers constructed on the basis of the reported fowl osteopontin nucleotide sequence. The PCR products from intact aortic smooth muscle and neointimal plaques correspond to the product from recombinant plasmid cDNA (a gift from Dr L. C. Gerstenfeld) transcribed in vitro. These results suggest that osteopontin is synthesized in intact aortic smooth muscle and neointimal plaques in fowl and that unmetabolized approximately 66 kDa osteopontin protein is a predominant form in the neointima, indicating that osteopontin protein may be actively synthesized in the neointima.

scholarly journals Extracellular Matrix Scaffold for Cardiac Repair

Circulation ◽  
2005 ◽  
Vol 112 (9_supplement) ◽  
Author(s):  
Keith A. Robinson ◽  
Jinshen Li ◽  
Megumi Mathison ◽  
Alka Redkar ◽  
Jianhua Cui ◽  
...  
Keyword(s):  
Heart Failure ◽  
Flow Cytometry ◽  
Extracellular Matrix ◽  
Smooth Muscle ◽  
Urinary Bladder ◽  
Smooth Muscle Actin ◽  
Left Ventricular ◽  
Foreign Body Response ◽  
Muscle Actin ◽  
Extracellular Matrix Scaffold

Background— Heart failure remains a significant problem. Tissue-engineered cardiac patches offer potential to treat severe heart failure. We studied an extracellular matrix scaffold for repairing the infarcted left ventricle. Methods and Results— Pigs (n=42) underwent left ventricular (LV) infarction. At 6 to 8 weeks, either 4-layer multilaminate urinary bladder-derived extracellular matrix or expanded polytetrafluoroethlyene (ePTFE) was implanted as full-thickness LV wall patch replacement. At 1-week, 1-month, or 3-month intervals, pigs were terminated. After macroscopic examination, samples of tissue were prepared for histology, immunocytochemistry, and analysis of cell proportions by flow cytometry. One-week and 1-month patches were intact with thrombus and inflammation; at 1 month, there was also tissue with spindle-shaped cells in proteoglycan-rich and collagenous matrix. More α-smooth muscle actin-positive cells were present in urinary bladder matrix (UBM) than in ePTFE (22.2±3.3% versus 8.4±2.7%; P =0.04). At 3 months, UBM was bioresorbed, and a collagen-rich vascularized tissue with numerous myofibroblasts was present. Isolated regions of α-sarcomeric actin-positive, intensely α-smooth muscle actin-immunopositive, and striated cells were observed. ePTFE at 3 months had foreign-body response with necrosis and calcification. Flow cytometry showed similarities of cells from UBM to normal myocardium, whereas ePTFE had limited cardiomyocyte markers. Conclusions— Appearance of a fibrocellular tissue that included contractile cells accompanied biodegradation of UBM when implanted as an LV-free wall infarction patch. UBM appears superior to synthetic material for cardiac patching and trends toward myocardial replacement at 3 months.

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