Mutational Analysis of the Central Centromere Targeting Domain of Human Centromere Protein C, (CENP-C)

2002 ◽  
Vol 275 (1) ◽  
pp. 81-91 ◽  
Author(s):  
Kang Song ◽  
Bobbi Gronemeyer ◽  
Wei Lu ◽  
Emily Eugster ◽  
John E. Tomkiel
Keyword(s):  
Protein C ◽  
Mutational Analysis ◽  
Human Centromere ◽  
Centromere Protein

scholarly journals Immunization with CENP-C Causes Aberrant Chromosome Segregation during Oocyte Meiosis in Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Jiao Fan ◽  
Yang Liu ◽  
Yiping Zhong
Keyword(s):  
Protein C ◽  
Polar Body ◽  
Control Group ◽  
Human Centromere ◽  
Centromere Protein ◽  
Oocyte Meiosis ◽  
First Polar Body ◽  
Freund’S Adjuvant ◽  
Freund's Adjuvant ◽  
Experimental Group

Anticentromere antibodies (ACA) were associated with lower oocyte maturation rates and cleavage rates, while the mechanism was not clear. Aims of this study were to examine whether active immunization with centromere protein C could elicit the CENP-C autoantibody in mice and the impacts of the CENP-C autoantibody on oocyte meiosis. Mice were divided into two groups, one was the experimental group immunized with human centromere protein C and Freund’s adjuvant (CFA), and the other was the control group injected with CFA only. Serum and oocytes of BALB/c mice immunized with human centromere protein C (CENP-C) in complete Freund’s adjuvant (CFA) or injected with only CFA were studied for the development of the CENP-C antibody. Rates of germinal vesicle breakdown (GVBD), first polar body (Pb1) extrusion, abnormal spindle morphology, and chromosome misalignment were compared between the experimental group and the control group. The CENP-C antibody was only observed in serum and oocytes of mice immunized with the centromere protein C antigen. The first polar body (Pb1) extrusion rate was lower in the experimental group ( P < 0.01 ). A higher percentage of spindle defects and chromosome congression failure were also detected in the experimental group (spindle defects: 64.67 ± 1.16 % vs. 9.27 ± 2.28 % control; chromosome misalignment: 50.80 ± 2.40 % vs. 8.30 ± 1.16 % control; P < 0.01 for both). Oocyte meiosis was severely impaired by the CENP-C antibody, which may be the main mechanism of adverse reproductive outcomes for ACA-positive women who have no clinical symptoms of any autoimmune diseases.

scholarly journals Cellular expression of human centromere protein C demonstrates a cyclic behavior with highest abundance in the G1 phase.

1996 ◽  
Vol 93 (19) ◽  
pp. 10234-10239 ◽  
Author(s):  
M. Knehr ◽  
M. Poppe ◽  
D. Schroeter ◽  
W. Eickelbaum ◽  
E. M. Finze ◽  
...  
Keyword(s):  
Protein C ◽  
Cyclic Behavior ◽  
G1 Phase ◽  
Human Centromere ◽  
Cellular Expression ◽  
Centromere Protein

The PxDLLCxE Sequence in Conserved Region 2 of Human Papilloma Virus 18 Protein E7 Is Required for E7 Binding to Centromere Protein C

Oncology ◽  
2012 ◽  
Vol 83 (4) ◽  
pp. 210-217 ◽  
Author(s):  
Yuji Yaginuma ◽  
Ayami Eguchi ◽  
Masafumi Yoshimoto ◽  
Katsuhiro Ogawa
Keyword(s):  
Human Papilloma Virus ◽  
Protein C ◽  
Papilloma Virus ◽  
Centromere Protein ◽  
Conserved Region

Mapping of the Human Centromere Protein B Gene (CENPB) to Chromosome 20p13 by Fluorescence in Situ Hybridization

Genomics ◽  
1994 ◽  
Vol 24 (1) ◽  
pp. 187-188 ◽  
Author(s):  
Naohiko Seki ◽  
Toshiyuki Saito ◽  
Katsumi Kitagawa ◽  
Hiroshi Masumoto ◽  
Tuneko Okazaki ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Human Centromere ◽  
Centromere Protein ◽  
Chromosome 20P13 ◽  
Protein B

scholarly journals Targeted disruption of mouse centromere protein C gene leads to mitotic disarray and early embryo death

1998 ◽  
Vol 95 (3) ◽  
pp. 1136-1141 ◽  
Author(s):  
P. Kalitsis ◽  
K. J. Fowler ◽  
E. Earle ◽  
J. Hill ◽  
K. H. A. Choo
Keyword(s):  
Protein C ◽  
Early Embryo ◽  
Targeted Disruption ◽  
Centromere Protein
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