Structural Polarity and Functional Bile Canaliculi in Rat Hepatocyte Spheroids

2002 ◽  
Vol 274 (1) ◽  
pp. 56-67 ◽  
Author(s):  
Susan Fugett Abu-Absi ◽  
Julie R. Friend ◽  
Linda K. Hansen ◽  
Wei-Shou Hu
Keyword(s):  
Bile Canaliculi ◽  
Rat Hepatocyte ◽  
Hepatocyte Spheroids

Structural and functional features of bile canaliculi in adult rat hepatocyte spheroids

2008 ◽  
Vol 16 (1) ◽  
pp. 61-66 ◽  
Author(s):  
Atsuko Uchida Yumoto ◽  
Sumio Watanabe ◽  
Miyoko Hirose ◽  
Tsuneo Kitamura ◽  
Yasushi Yamaguchi ◽  
...  
Keyword(s):  
Bile Canaliculi ◽  
Rat Hepatocyte ◽  
Adult Rat ◽  
Functional Features ◽  
Hepatocyte Spheroids

Three-dimensional co-culture of rat hepatocyte spheroids and NIH/3T3 fibroblasts enhances hepatocyte functional maintenance

2005 ◽  
Vol 1 (4) ◽  
pp. 399-410 ◽  
Author(s):  
Hong-Fang Lu ◽  
Kian-Ngiap Chua ◽  
Peng-Chi Zhang ◽  
Wei-Seng Lim ◽  
Seeram Ramakrishna ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Rat Hepatocyte ◽  
3T3 Fibroblasts ◽  
Hepatocyte Spheroids ◽  
Nih 3T3

scholarly journals Analysis of hepatocyte plasma membrane domains during rat development using monoclonal antibodies.

1988 ◽  
Vol 36 (1) ◽  
pp. 87-94 ◽  
Author(s):  
A Moreau ◽  
M Maurice ◽  
G Feldmann
Keyword(s):  
Monoclonal Antibodies ◽  
Plasma Membrane ◽  
Major Part ◽  
Post Partum ◽  
Membrane Domains ◽  
Bile Canaliculi ◽  
Rat Hepatocyte ◽  
Adult Rat ◽  
Liver Functions ◽  
Plasma Membrane Domains

The plasma membrane of adult rat hepatocyte consists of three domains, which have been identified by the monoclonal antibodies A39 and A59 as markers of the sinusoidal domain, B1 of the lateral, and B10 of the canalicular domains (Eur J Cell Biol 39:122, 1985). These monoclonal antibodies were used to study, by indirect immunocytochemistry, formation of the hepatocyte plasma membrane domains during development, from day 15 of gestation to day 35 post partum. The antigens defined by A39, B1, and B10 were detected, from day 15, over the major part of the hepatocyte plasma membrane except for the membranes of newly formed bile canaliculi, which were not labeled by B1 and only poorly labeled, if at all, by A39 and B10. As soon as fetuses were 16 days old, B1 labeled predominantly the lateral domain, as in the adult. Labeling with B10 progressively intensified on the membranes of bile canaliculi, but localization was not exclusively canalicular until day 21 post partum. A39 intensely labeled the canalicular membranes at 19-21 days of gestation, while at 35 days post partum it exhibited the predominantly sinusoidal labeling observed in adult hepatocytes. The antigen defined by A59 was not detected before birth and was found exclusively on the sinusoidal domain, as in the adult. These results show that the patterns of antigen distribution on different plasma membrane domains establish themselves at different rates. The marked differences observed between fetal or neonatal and adult hepatocytes might be responsible for immaturity of liver functions in the neonate.

Fluorometric assessment of acetaminophen-induced toxicity in rat hepatocyte spheroids seeded on micro-space cell culture plates

2014 ◽  
Vol 28 (6) ◽  
pp. 1176-1182 ◽  
Author(s):  
Seigo Sanoh ◽  
Masataka Santoh ◽  
Masashi Takagi ◽  
Tatsuya Kanayama ◽  
Kazumi Sugihara ◽  
...  
Keyword(s):  
Cell Culture ◽  
Rat Hepatocyte ◽  
Hepatocyte Spheroids ◽  
Space Cell

scholarly journals Preparation of Primary Rat Hepatocyte Spheroids Utilizing the Liquid‐Overlay Technique

2019 ◽  
Vol 81 (1) ◽  
Author(s):  
Jonathan A. Kyffin ◽  
Christopher R. Cox ◽  
Joseph Leedale ◽  
Helen E. Colley ◽  
Craig Murdoch ◽  
...  
Keyword(s):  
Rat Hepatocyte ◽  
Overlay Technique ◽  
Hepatocyte Spheroids

Cold Storage of Rat Hepatocyte Spheroids

2014 ◽  
Vol 23 (7) ◽  
pp. 819-830 ◽  
Author(s):  
Hongling Liu ◽  
Yue Yu ◽  
Jaime Glorioso ◽  
Shennen Mao ◽  
Brian Rodysil ◽  
...  
Keyword(s):  
Cold Storage ◽  
Rat Hepatocyte ◽  
Hepatocyte Spheroids
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