scholarly journals Type IV Collagen Induces Matrix Metalloproteinase 2 Activation in HT1080 Fibrosarcoma Cells

2000 ◽  
Vol 261 (2) ◽  
pp. 348-359 ◽  
Author(s):  
Erik Maquoi ◽  
Francis Frankenne ◽  
Agnès Noël ◽  
Hans-Willi Krell ◽  
Frank Grams ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Type Iv Collagen ◽  
Matrix Metalloproteinase 2 ◽  
Type Iv ◽  
Fibrosarcoma Cells

scholarly journals Canstatin stimulates migration of rat cardiac fibroblasts via secretion of matrix metalloproteinase-2

2017 ◽  
Vol 312 (3) ◽  
pp. C199-C208 ◽  
Author(s):  
Muneyoshi Okada ◽  
Naoki Murata ◽  
Hideyuki Yamawaki
Keyword(s):  
Cell Migration ◽  
Matrix Metalloproteinase ◽  
Type Iv Collagen ◽  
Cardiac Fibroblasts ◽  
Fiber Formation ◽  
Matrix Metalloproteinase 2 ◽  
Actin Stress Fiber ◽  
Adult Rat ◽  
Type Iv ◽  
Erk Phosphorylation

Type IV collagen, a nonfibrillar type, is ubiquitously expressed in the basement membrane around cardiomyocytes. Canstatin, a cleaved product of α2 chain of type IV collagen, is an antiangiogenic factor. Because it has not been clarified whether canstatin exerts other biological activities in heart, we investigated the effects of canstatin on adult rat cardiac fibroblasts. Cell migration was determined by Boyden chamber assay. Western blotting was performed to detect secretion of matrix metalloproteinase-2 (MMP-2) and MMP-9 and phosphorylation of extracellular signal-regulated kinase (ERK). Localization of MMP-2 was detected by immunofluorescence staining. Canstatin (250 ng/ml) significantly increased migration, secretion, and activity of MMP-2 but not MMP-9. CTTHWGFTLC peptide, an MMP inhibitor and small interfering RNA (siRNA) against MMP-2 suppressed the canstatin-induced (250 ng/ml, 24 h) migration. Canstatin (250 ng/ml, 30 min) significantly increased phosphorylation of ERK. PD98059, a MEK inhibitor, significantly suppressed the canstatin-induced (250 ng/ml, 24 h) migration but not secretion of MMP-2. An increase in MMP-2 expression was observed in cytoplasm of the canstatin-treated (250 ng/ml) cardiac fibroblasts (within 30 min). Canstatin induced actin stress fiber formation, which was inhibited by Y-27632, a Rho-associated kinase inhibitor. Y-27632 also suppressed the canstatin-induced (250 ng/ml, 24 h) MMP-2 secretion. Canstatin (250 ng/ml, 30 min) failed to induce ERK phosphorylation in MMP-2 siRNA-treated cardiac fibroblasts. In conclusion, this study revealed a novel function of canstatin for inducing cell migration of adult rat cardiac fibroblasts at least in part by ERK phosphorylation through MMP-2 secretion, possibly via actin cytoskeletal change.

Matrix metalloproteinase 2 secretion in WEHI 164 fibrosarcoma cells is nitric oxide-related and modified by morphine

2006 ◽  
Vol 530 (1-2) ◽  
pp. 33-39 ◽  
Author(s):  
Ahmad Shariftabrizi ◽  
Artemissia-Phoebe Nifli ◽  
Mohammad Ansari ◽  
Farshid Saadat ◽  
Mohammad Reza Ebrahimkhani ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Matrix Metalloproteinase ◽  
Matrix Metalloproteinase 2 ◽  
Fibrosarcoma Cells

scholarly journals Enhanced Expression of Type IV Collagen-binding Protein (p29) inFyn-transfected Murine Fibrosarcoma Cells

2002 ◽  
Vol 93 (10) ◽  
pp. 1090-1099 ◽  
Author(s):  
Kazuhiko Koike ◽  
Katsuhisa Kogawa ◽  
Tetsuji Takayama ◽  
Naohito Yoshizaki ◽  
Hirohito Muramatsu ◽  
...  
Keyword(s):  
Type Iv Collagen ◽  
Binding Protein ◽  
Collagen Binding ◽  
Type Iv ◽  
Fibrosarcoma Cells ◽  
Enhanced Expression ◽  
Murine Fibrosarcoma

scholarly journals Modulation of 72-Kilodalton Type IV Collagenase (Matrix Metalloproteinase-2) by Ascorbic Acid in Cultured Human Amnion-Derived Cells1

1998 ◽  
Vol 59 (2) ◽  
pp. 326-329 ◽  
Author(s):  
Frania Pfeffer ◽  
Esther Casanueva ◽  
Jacobo Kamar ◽  
Alejandra Guerra ◽  
Otilia Perichart ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Matrix Metalloproteinase ◽  
Matrix Metalloproteinase 2 ◽  
Type Iv Collagenase ◽  
Human Amnion ◽  
Type Iv

scholarly journals Nonenzymatic glycation of type IV collagen and matrix metalloproteinase susceptibility

1997 ◽  
Vol 52 (5) ◽  
pp. 1302-1312 ◽  
Author(s):  
Joni D. Mott ◽  
Raja G. Khalifah ◽  
Hideaki Nagase ◽  
Charles F. Shield ◽  
Julie K. Hudson ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Type Iv Collagen ◽  
Nonenzymatic Glycation ◽  
Type Iv
Sign in / Sign up

Export Citation Format

Share Document